How Does pH Fit in with Oscillating Polar Growth?

Mangano, Silvina; Pacheco, Javier Martínez; Marino-Buslje, Cristina; Estevez, José M.

doi:10.1016/j.tplants.2018.02.008

Cited by 37 publications

(27 citation statements)

References 87 publications

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“…The activation of AHAs, for example, is known to require the phosphorylation of their penultimate C‐terminal threonine residue (Inoue & Kinoshita, ). Moreover, rapid adjustment of cell wall/apoplastic pH can influence cell wall loosening and stiffening, and thus the connection of the cell wall to the underlying cell, although the exact mechanism is not yet fully understood (Fendrych et al , ; Barbez et al , ; Mangano et al , ). Plant microbes were shown to secrete various cell wall‐degrading enzymes depending on the ambient pH (Li et al , ).…”

Section: Introductionmentioning

confidence: 99%

Pathogen‐inducedpHchanges regulate the growth‐defense balance in plants

et al. 2019

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Environmental adaptation of organisms relies on fast perception and response to external signals, which lead to developmental changes. Plant cell growth is strongly dependent on cell wall remodeling. However, little is known about cell wall‐related sensing of biotic stimuli and the downstream mechanisms that coordinate growth and defense responses. We generated genetically encoded pH sensors to determine absolute pH changes across the plasma membrane in response to biotic stress. A rapid apoplastic acidification by phosphorylation‐based proton pump activation in response to the fungus Fusarium oxysporum immediately reduced cellulose synthesis and cell growth and, furthermore, had a direct influence on the pathogenicity of the fungus. In addition, pH seems to influence cellulose structure. All these effects were dependent on the COMPANION OF CELLULOSE SYNTHASE proteins that are thus at the nexus of plant growth and defense. Hence, our discoveries show a remarkable connection between plant biomass production, immunity, and pH control, and advance our ability to investigate the plant growth‐defense balance.

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Section: Introductionmentioning

confidence: 99%

Pathogen‐inducedpHchanges regulate the growth‐defense balance in plants

et al. 2019

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“…For example, the activation of AHAs is known to require the phosphorylation of their penultimate C-terminal threonine residue (Inoue & Kinoshita, 2017) . Moreover, rapid adjustment of the CW/apoplastic pH can influence CW loosening/stiffening, and thus the connection of the CW to the underlying cell (Fendrych et al , 2016;Barbez et al , 2017) by a mechanism which is not yet fully understood (Mangano et al , 2018) . Interestingly, plant microbes have been shown to secrete various CW degrading enzymes depending on the apoplastic pH (pH apo ) of their host (Li et al , 2012) .…”

Section: Introductionmentioning

confidence: 99%

Pathogen-induced pH changes regulate the growth-defense balance of plants

Kesten

Gámez-Arjona

Scholl

et al. 2019

Preprint

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Environmental adaptation of organisms relies on fast perception and response to external signals, which lead to developmental changes. Plant cell growth is strongly dependent on cell wall remodeling. However, little is known about cell wall-related sensing of biotic stimuli and the downstream mechanisms that coordinate growth and defense responses. We generated genetically encoded pH sensors to determine absolute pH changes across the plasma membrane in response to biotic stress. A rapid apoplastic acidification by phosphorylation-based proton pump activation was followed by an acidification of the cortical side of the plasma membrane in response to the fungus Fusarium oxysporum. The proton chemical gradient modulation immediately reduced cellulose synthesis and cell growth and, furthermore, had a direct influence on the pathogenicity of the fungus. All these effects were dependent on the COMPANION OF CELLULOSE SYNTHASE proteins that are thus at the nexus of plant growth and defense. Hence, our discoveries show a remarkable connection between plant biomass production, immunity, and pH control, and advance our ability to investigate the plant growth-defense balance. We are grateful to G. Sancho-Andrés, B. Pfister, and P. van Dam for technical support. We thank S. Persson for the cc1cc2 , cc1cc2 GFP-CC1 and cc1cc2 GFP-CC1ΔN120 lines and A. Molina, G. Bissoli and E. López-Solanilla for constructive discussions. Live cell imaging was performed with equipment maintained by

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“…The effect of ROS on PM H + -ATPases is not well known but may not be transcriptional in our model,as no change in the level of AHA5-like gene transcript was observed in MV treated D sunflower seeds [1]. However, the H + transport activity of AHAs could be regulated by the cytoplasmic redox state which is linked to ROS [50]. The effects of the ROS pharmacology on dormancy demonstrated MV-stimulated dormancy alleviation and germination delay by inhibitors of superoxide generation, confirming the link between the seed cell polarization and its ability to germinate reinforcing the hypothesis of a role in regulation of PM H + -ATPase during dormancy alleviation.…”

Section: Discussionmentioning

confidence: 80%

Activation of plasma membrane H+-ATPases participates in dormancy alleviation in sunflower seeds

Bont¹,

Naim²,

Arbelet-Bonnin

et al. 2019

Plant Science

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Using various inhibitors and scavengers we took advantage of the size of sunflower (Helianthus annuus) seeds to investigate in vivo the effects of hormones, namely abscisic acid (ABA) and ethylene (ET), and reactive oxygen species (ROS) on the polarization of dormant (D) and non-dormant (ND) embryonic seed cells using microelectrodes. Our data show that D and ND seed cells present different polarization likely due to the regulation of plasma membrane (PM) H +-ATPase activity. The data obtained after addition of hormones or ROS scavengers further suggest that ABA dependent inhibition of PM H +-ATPases could participate in dormancy maintenance and that ET-and ROS-dependent PM H +-ATPase stimulation could participate in dormancy release in sunflower seeds.

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How Does pH Fit in with Oscillating Polar Growth?

Cited by 37 publications

References 87 publications

Pathogen‐inducedpHchanges regulate the growth‐defense balance in plants

Pathogen‐inducedpHchanges regulate the growth‐defense balance in plants

Pathogen-induced pH changes regulate the growth-defense balance of plants

Activation of plasma membrane H+-ATPases participates in dormancy alleviation in sunflower seeds

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