How does metabolic rate in plant shoot tips change after cryopreservation?

Whelehan, Lily M.; Dalziell, Emma L.; Bunn, Eric; Mancera, Ricardo L.; Funnekotter, Bryn

doi:10.1016/j.cryobiol.2022.10.003

Cited by 7 publications

(7 citation statements)

References 48 publications

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“…The oxidative stress gradually increases from the PC, OP, and CP and peaks at C/W (LN), the unloading stage [71,100,[103][104][105], and exogenous antioxidants improved LN regeneration [99,100,106,107]. Metabolic rates are the lowest at this stage [108], while total organic acid contents have jumped [105]. Among the 14 organic acids (OAs) tested, four OA (2-hydroxybutyric, 3-hydroxypropionic, lactic, and glycolic) contents were highly correlated (r = 0.980) with the CPA concentration employed.…”

Section: Three-step Regrowth Initially With Ammonium-free Mediummentioning

confidence: 99%

Optimizing the Droplet-Vitrification Procedure by Balancing the Cryoprotection and Cytotoxicity of Alternative Plant Vitrification Solutions Based on the Nature of Donor Plant Vigor

Kim

2023

Plants

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Over 30 years of plant vitrification, droplet vitrification (DV) of in vitro propagules and slow freezing of dormant buds are typical methods of large-scale cryobanking worldwide. One-step sucrose preculture and Plant Vitrification Solution 2 (PVS2) cryoprotection in solution-based vitrification often face unacceptably low regeneration, and the results are on a case-by-case basis depending on the plant species, like a blind test. The absence of a universal protocol applicable across all plant diversity is considered one of the limiting factors. For wild flora, limits of source material available and difficulties in in vitro propagation make it worse to re-optimize the protocol steps for new species. Since cryoprotectant toxicity is the most crucial barrier to the vitrification of organized explants, selecting alternative plant vitrification solutions (PVS) based on the cytotoxicity of cryoprotectants is vital. This review proposes the concept of donor plant vigor (DPV), which refers to the donor plant properties that determine the potential to regenerate normal plantlets under various cryopreservation procedures. DV is a multi-stage procedure with many factors from stage (1) material preparation to (2) pre-liquid nitrogen (pre-LN) (preculture, osmoprotection, cryoprotection), (3) LN (cooling), (4) warming conditions (rewarming, unloading), and (5) regrowth. Since the cytotoxicity of PVS is a primary limiting factor in DV approaches, DPV is crucial for coping with the toxicity of PVS. The DPV is innate and can be maximized with appropriate material preparations, i.e., vigorously growing in subcultures aided by a liquid overlay on top of the gelled medium, selecting proper explants, optimizing the two-step preculture conditions, and media supplements. Developing the DV protocol starts with testing the material with a tentative standard protocol, which includes a two-step preculture (10% sucrose for 31 h and 17.5% sucrose for 16 h), osmoprotection with C4-35%, cryoprotection with A3-80% (60 min at 0 °C), cooling, and rewarming using aluminum foil strips. Using a three-step regrowth initially with ammonium-free regrowth medium, regrowth of shoot tips in one plate following the successive stages of the tentative standard protocol for shoot tips, i.e., fresh, PC, OP, CP (LNC), and LN, is a valuable tool to characterize the sensitivity of the material and to standardize the procedure by tuning the cryoprotection and cytotoxicity of cryoprotectants. A-series PVS (A3-90%, A3-80%, A3-70%) and B-series PVS (PVS3, B5-85%) can be tested based on the DPV. These alternative PVSs have been applied in over 30 pieces of literature with an 8.5~67.3% increase in LN regeneration compared to PVS2 and Plant Vitrification Solution 3 (PVS3) treatments. Using this approach as an alternative to blind condition screening would be influential in broadening the cryopreservation of diverse wild species and problem materials.

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Section: Three-step Regrowth Initially With Ammonium-free Mediummentioning

confidence: 99%

Optimizing the Droplet-Vitrification Procedure by Balancing the Cryoprotection and Cytotoxicity of Alternative Plant Vitrification Solutions Based on the Nature of Donor Plant Vigor

Kim

2023

Plants

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“…8 Certain plant species may expand into new habitats as minimum temperatures rise (Bradley, Wilcove, and Oppenheimer 2010). Elevated minimum temperatures can also slightly increase plant metabolic rates, benefiting processes like nutrient uptake and photosynthesis when they stay within the plant's optimal range (Whelehan et al 2022;Hussain et al 2019). 9 The per capita water consumption in Israel dwarfs that of Palestinians, surpassing it by at least fourfold, with certain settlements in the West Bank consuming an astonishing 21 times more water per capita than neighboring Palestinian communities (Al-Haq and EWASH 2011).…”

Section: Climate Change and Water Security In Palestinementioning

confidence: 99%

The impact of climate change on the Palestinian sectoral reallocation of labor

Hallaq,

Daas

2024

International Review of Applied Economics

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The research leverages yearly variations in climate variables, such as rainfall and temperature, across the West Bank from 1999 to 2018 to assess their influence on individuals' decisions to stay in the agricultural sector. The main findings suggest that an increase in rainfall in the previous year is associated with a higher proportion of workers in the agricultural sector, especially in regions where agriculture is the primary economic activity. Temperature variation is also an important factor. An increase in the maximum temperature will generally have a negative effect on the supply of labor in the agricultural sector, while an increase in the minimum temperature may have a positive effect. However, this effect varies across different regions of the West Bank, reflecting the diverse agricultural practices and irrigation methods employed. The study also examines two potential mechanisms through which climate change affects labor decisions: agricultural labor migration to the Israeli labor market and how climate shocks affect agricultural wages.

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“…The first cryo-collections of plant genetic resources appeared in global genebanks and research centers in the 1970s–1980s, and mostly functioned as a secure long-term backup for the materials maintained in active collections in vitro and in the fields [ 23 , 27 ]. Although introducing plant material to a cryobank is costly and laborious, it pays off over the years due to lower costs of maintenance and, most importantly, reduced chances for morphological, biosynthetic, and genetic variations in the specimens [ 119 , 120 , 121 ].…”

Section: Ippras Cryobank Of Plant Genetic Resourcesmentioning

confidence: 99%

Plants, Cells, Algae, and Cyanobacteria In Vitro and Cryobank Collections at the Institute of Plant Physiology, Russian Academy of Sciences—A Platform for Research and Production Center

Yuorieva,

Sinetova,

Messineva

et al. 2023

Biology

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Ex situ collections of algae, cyanobacteria, and plant materials (cell cultures, hairy and adventitious root cultures, shoots, etc.) maintained in vitro or in liquid nitrogen (−196 °C, LN) are valuable sources of strains with unique ecological and biotechnological traits. Such collections play a vital role in bioresource conservation, science, and industry development but are rarely covered in publications. Here, we provide an overview of five genetic collections maintained at the Institute of Plant Physiology of the Russian Academy of Sciences (IPPRAS) since the 1950–1970s using in vitro and cryopreservation approaches. These collections represent different levels of plant organization, from individual cells (cell culture collection) to organs (hairy and adventitious root cultures, shoot apices) to in vitro plants. The total collection holdings comprise more than 430 strains of algae and cyanobacteria, over 200 potato clones, 117 cell cultures, and 50 strains of hairy and adventitious root cultures of medicinal and model plant species. The IPPRAS plant cryobank preserves in LN over 1000 specimens of in vitro cultures and seeds of wild and cultivated plants belonging to 457 species and 74 families. Several algae and plant cell culture strains have been adapted for cultivation in bioreactors from laboratory (5–20-L) to pilot (75-L) to semi-industrial (150–630-L) scale for the production of biomass with high nutritive or pharmacological value. Some of the strains with proven biological activities are currently used to produce cosmetics and food supplements. Here, we provide an overview of the current collections’ composition and major activities, their use in research, biotechnology, and commercial application. We also highlight the most interesting studies performed with collection strains and discuss strategies for the collections’ future development and exploitation in view of current trends in biotechnology and genetic resources conservation.

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How does metabolic rate in plant shoot tips change after cryopreservation?

Cited by 7 publications

References 48 publications

Optimizing the Droplet-Vitrification Procedure by Balancing the Cryoprotection and Cytotoxicity of Alternative Plant Vitrification Solutions Based on the Nature of Donor Plant Vigor

Optimizing the Droplet-Vitrification Procedure by Balancing the Cryoprotection and Cytotoxicity of Alternative Plant Vitrification Solutions Based on the Nature of Donor Plant Vigor

The impact of climate change on the Palestinian sectoral reallocation of labor

Plants, Cells, Algae, and Cyanobacteria In Vitro and Cryobank Collections at the Institute of Plant Physiology, Russian Academy of Sciences—A Platform for Research and Production Center

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