How Does European Mistletoe Survive Without Complex I?

Fonseca‐Pereira, Paula da; Silva, Willian Batista; Araújo, Wagner L.; Nunes‐Nesi, Adriano

doi:10.1016/j.tplants.2018.07.008

Cited by 6 publications

(3 citation statements)

References 12 publications

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“…Unlike its chloroplast counterpart, lack of this complex affects carbon metabolism and photosynthesis (Fromm et al ), and entails profound changes leading to curled leaves and delayed reproductive and vegetative development phenotype (de Longevialle et al ). Surprisingly, recent studies have found that hemiparasitic mistletoe has lost the ability to produce a functional Complex I (da Fonseca‐Pereira et al ) pointing to more similarities to the NDH complex.…”

Section: Nadh Dehydrogenase: the Main Target?mentioning

confidence: 99%

PPR proteins – orchestrators of organelle RNA metabolism

Rovira

Smith

2019

Physiologia Plantarum

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Pentatricopeptide repeat (PPR) proteins are important RNA regulators in chloroplasts and mitochondria, aiding in RNA editing, maturation, stabilisation or intron splicing, and in transcription and translation of organellar genes. In this review, we summarise all PPR proteins documented so far in plants and the green alga Chlamydomonas. By further analysis of the known target RNAs from Arabidopsis thaliana PPR proteins, we find that all organellar‐encoded complexes are regulated by these proteins, although to differing extents. In particular, the orthologous complexes of NADH dehydrogenase (Complex I) in the mitochondria and NADH dehydrogenase‐like (NDH) complex in the chloroplast were the most regulated, with respectively 60 and 28% of all characterised A. thaliana PPR proteins targeting their genes.

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Section: Nadh Dehydrogenase: the Main Target?mentioning

confidence: 99%

PPR proteins – orchestrators of organelle RNA metabolism

Rovira

Smith

2019

Physiologia Plantarum

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“…While it initially could not be excluded that the corresponding genes had been overlooked (due to far-going sequence alterations during evolution or translocation of sequences to the nuclear genome), it later became clear by proteome analyses of isolated mitochondria that complex I indeed is absent in V. album (Maclean et al, 2018;Senkler et al, 2018). This was a surprising finding, because it is the only known example of a multicellular eukaryote that can carry out cellular respiration in the absence of complex I (Busch, 2018;da Fonseca-Pereira et al, 2018). In V. album, complexes III and IV of the respiratory chain form stable supercomplexes; furthermore, Proteome analyses of V. album mitochondria were so far greatly hindered due to the very limited genome sequence information for V. album or any other species of the Viscum genus or the Santalaceae family (which includes the Viscum genus and several related genera).…”

Section: Transcripts Encoding V Album Proteins Localized In the Mitochondriamentioning

confidence: 99%

The gene space of European mistletoe (Viscum album)

et al. 2021

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European mistletoe (Viscum album) is a hemiparasitic flowering plant that is known for its very special life cycle and extraordinary biochemical properties. Particularly, V. album has an unusual mode of cellular respiration that takes place in the absence of mitochondrial complex I. However, insights into the molecular biology of V. album so far are very limited. Since the genome of V. album is extremely large (estimated 600 times larger than the genome of the model plant Arabidopsis thaliana) it has not been sequenced up to now. We here report sequencing of the V. album gene space (defined as the space including and surrounding genic regions, encompassing coding as well as 5 0 and 3 0 non-coding regions). mRNA fractions were isolated from different V. album organs harvested in summer or winter and were analyzed via single-molecule real-time sequencing. We determined sequences of 39 092 distinct open reading frames encoding 32 064 V. album proteins (designated V. album protein space). Our data give new insights into the metabolism and molecular biology of V. album, including the biosynthesis of lectins and viscotoxins. The benefits of the V. album gene space information are demonstrated by re-evaluating mass spectrometry-based data of the V. album mitochondrial proteome, which previously had been evaluated using the A. thaliana genome sequence. Our re-examination allowed the additional identification of nearly 200 mitochondrial proteins, including four proteins related to complex I, which all have a secondary function not related to respiratory electron transport. The V. album gene space sequences are available at the NCBI.

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“…How can V. album cope with a reduced capacity for generating mitochondrial ATP? This question has been intensively discussed ( Busch, 2018 ; da Fonseca-Pereira et al, 2018 ; Maclean et al, 2018 ; Senkler et al, 2018 ). One of the most ATP-consuming processes in the cytosol of V. album leaf cells is the synthesis of sucrose from UDP-glucose and fructose (formation of UDP glucose requires UTP, which is synthesized by UDP phosphorylation using ATP).…”

Section: Introductionmentioning

confidence: 99%

The photosynthesis apparatus of European mistletoe (Viscum album)

et al. 2022

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European mistletoe (Viscum album) is known for its special mode of cellular respiration. It lacks the mitochondrial NADH dehydrogenase complex (complex I of the respiratory chain) and has restricted capacities to generate mitochondrial adenosine triphosphate (ATP). Here, we present an investigation of the V. album energy metabolism taking place in chloroplasts. Thylakoids were purified from young V. album leaves, and membrane bound protein complexes were characterized by Blue native polyacrylamide gel electrophoresis as well as by the complexome profiling approach. Proteins were systematically identified by label-free quantitative shotgun proteomics. We identified > 1800 distinct proteins (accessible at https://complexomemap.de/va_leaves), including nearly 100 proteins forming part of the protein complexes involved in the light-dependent part of photosynthesis. The photosynthesis apparatus of V. album has distinct features: (i) comparatively low amounts of photosystem I; (ii) absence of the NDH complex (the chloroplast pendant of mitochondrial complex I involved in cyclic electron transport around photosystem I); (iii) reduced levels of the proton gradient regulation 5 (PGR5) and proton gradient regulation 5-like 1 (PGRL1) proteins, which offer an alternative route for cyclic electron transport around photosystem I; (iv) comparable amounts of photosystem II and the chloroplast ATP synthase complex to other seed plants. Our data suggest a restricted capacity for chloroplast ATP biosynthesis by the photophosphorylation process. This is in addition to the limited ATP supply by the mitochondria. We propose a view on mistletoe's mode of life, according to which its metabolism relies to a greater extent on energy-rich compounds provided by the host trees.

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How Does European Mistletoe Survive Without Complex I?

Cited by 6 publications

References 12 publications

PPR proteins – orchestrators of organelle RNA metabolism

PPR proteins – orchestrators of organelle RNA metabolism

The gene space of European mistletoe (Viscum album)

The photosynthesis apparatus of European mistletoe (Viscum album)

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