How does chronic temperature exposure affect hypoxia tolerance in sheepshead minnows’ (Cyprinodon variegatus variegatus) ability to tolerate oxidative stress?

Jiménez, Ana Gabriela; Braun, Evan; Tobin, Kailey J.

doi:10.1007/s10695-018-0583-0

Cited by 13 publications

(1 citation statement)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many studies have shown that reactive oxygen species (ROS) are overproduced in fish under hypoxic conditions. The superoxide anion radical can be decomposed into H2O2, which is less harmful, via the activity of SOD, and then CAT decomposes H2O2 into H2O and O2 through the cellular antioxidant pathway [38]. Therefore, the activities of CAT and SOD reflect the defense function of the antioxidant system to some extent.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and 16S rRNA Analyses Reveal That Hypoxic Stress Affects the Antioxidant Capacity of Largemouth Bass (Micropterus salmoides), Resulting in Intestinal Tissue Damage and Structural Changes in Microflora

Song

Tao

et al. 2022

Antioxidants

View full text Add to dashboard Cite

Dissolved oxygen (DO) is a key factor affecting the health of aquatic organisms in an intensive aquaculture environment. In this study, largemouth bass (Micropterus salmoides) were subjected to acute hypoxic stress for 96 h (DO: 1.00 mg/L) followed by recovery under sufficient DO conditions (DO: 7.50 mg/L) for 96 h. Serum biochemical indices, intestinal histomorphology, the transcriptome, and intestinal microbiota were compared between hypoxia-treated fish and those in a control group. The results showed that hypoxia caused oxidative stress, exfoliation of the intestinal villus epithelium and villus rupture, and increased cell apoptosis. Transcriptome analyses revealed that antioxidant-, inflammation-, and apoptosis-related pathways were activated, and that the MAPK signaling pathway played an important role under hypoxic stress. In addition, 16S rRNA sequencing analyses revealed that hypoxic stress significantly decreased bacterial richness and identified the dominant phyla (Proteobacteria, Firmicutes) and genera (Mycoplasma, unclassified Enterobacterales, Cetobacterium) involved in the intestinal inflammatory response of largemouth bass. Pearson’s correlation analyses showed that differentially expressed genes in the MAPK signaling pathway were significantly correlated with some microflora. The results of this study will help to develop strategies to reduce damage caused by hypoxic stress in aquacultured fish.

show abstract

Section: Discussionmentioning

confidence: 99%