How do wettability, zeta potential and hydroxylation degree affect the biological response of biomaterials?

Spriano, Silvia Maria; Chandra, Vimlesh; Cochis, Andrea; Uberti, Francesca; Rimondini, Lia; Bertone, Elisa; Vitale, Alessandra; Scolaro, Cristina; Ferrari, Michele; Cirisano, Francesca; Confiengo, Giovanna Gautier di; Ferraris, Sara

doi:10.1016/j.msec.2016.12.107

Cited by 121 publications

(64 citation statements)

References 61 publications

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“…Mechanical anchorage to surface topographical cues, surface energy and wettability are known as factors influencing cell adhesion. In the present study, our results clearly revealed that the hydrophobic disks (D56 disks) had a significantly reduced number of adherent cells compared with the hydrophilic disks (D0 disks), which was consistent with some previous studies 20, [24][25][26] . These results illustrate one of the positive characteristics of hydrophilic surfaces and suggest that the positive effects of hydrophilic surfaces shown in previous studies can be attributed to enhanced cell adhesion capacity and the resulting cellular interactions.…”

Section: Discussionsupporting

confidence: 93%

Influence of the wettability of different titanium surface topographies on initial cellular behavior

et al. 2018

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This study examined the influence of the time-dependent wettability of different surface topographies on initial cellular behavior. Titanium disks with smooth topography (SM) and three kinds of rough topography (sandblasted (SA), microtopography (M) and nanotopography (N)) were prepared. Time-dependent changes in surface wettability were observed in all surfaces as shown in previous studies. On SM surfaces, hydrophobic alteration influenced cell spreading and the activity of RhoA (a small GTPase protein of the Rho family), while no alterations were observed on rough surfaces except for the number of adherent cells. Serum adsorption could recover these functional deteriorations by hydrophobic alteration. These findings suggest that surface topography is a more potent regulator in initial cellular behaviors such as cell spreading and RhoA activation than surface wettability.

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Section: Discussionsupporting

confidence: 93%

Influence of the wettability of different titanium surface topographies on initial cellular behavior

et al. 2018

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“…On the other hand, zeta potential measurements on solid surfaces are less usual. Contact angle titatration has been proposed for the evaluation of the isoelectric point of solid samples (Chedov and Logan, 2004), but the result is limited to the IEP value and measurements are quite complex with consequent difficulties in the obtainment of reliable results (Spriano et al, 2017). Electrokinetic measurements of the zeta potential of solid surfaces can be more interesting for the determination of both the IEP value and also of the zeta potential in function of pH, making possible a more in depth understanding of surface properties (Cai et al, 2006; Luxbacher, 2014; Spriano et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Zeta Potential Measurements on Solid Surfaces for in Vitro Biomaterials Testing: Surface Charge, Reactivity Upon Contact With Fluids and Protein Absorption

Ferraris

Cazzola

Peretti

et al. 2018

Front. Bioeng. Biotechnol.

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Surface properties of biomaterials (e.g., roughness, chemical composition, charge, wettability, and hydroxylation degree) are key features to understand and control the complex interface phenomena that happens upon contact with physiological fluids. Numerous physico-chemical techniques can be used in order to investigate in depth these crucial material features. Among them, zeta potential measurements are widely used for the characterization of colloidal suspensions, but actually poorly explored in the study of solid surfaces, even if they can give significant information about surface charge in function of pH and indirectly about surface functional groups and reactivity. The aim of the present research is application of zeta potential measurements of solid surfaces for the in vitro testing of biomaterials. In particular, bare and surface modified Ti6Al4V samples have been compared in order to evaluate their isoelectric points (IEPs), surface charge at physiological pH, in vitro bioactivity [in simulated body fluid (SBF)] and protein absorption. Zeta potential titration was demonstrated as a suitable technique for the surface characterization of surface treated Ti6Al4V substrates. Significant shift of the isoelectric point was recorded after a chemical surface treatment (because of the exposition of hydroxyl groups), SBF soaking (because of apatite precipitation IEP moves close to apatite one) and protein absorption (IEP moves close to protein ones). Moreover, the shape of the curve gives information about exposed functional groups (e.g., a plateau in the basic range appears due to the exposition of acidic OH groups and in the acidic range due to exposition of basic NH2 groups).

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“…The theory suggests that calcium ions (Ca 2+ ) can act as a bridge between the acquired membrane and the bacteria. However, it should be noted that some scholars have pointed out that the effect of calcium ions on bacterial adhesion cannot be explained by simple electrostatic attraction, and adhesion and calcium ion concentration are not simply positively or negatively correlated.…”

Section: Antibiofilm Mechanisms Of Nanoparticles At Different Stages mentioning

confidence: 99%

Nanoparticles for the Treatment of Oral Biofilms: Current State, Mechanisms, Influencing Factors, and Prospects

Wang

Yuan

et al. 2019

Adv Healthcare Materials

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Due to their excellent size, designability, and outstanding targeted antibacterial effects, nanoparticles have become a potential option for controlling oral biofilm‐related infections. However, the formation of an oral biofilm is a dynamic process, and factors affecting the performance of antibiofilm treatments are complex. As such, when examining the existing literature on the antibiofilm effects of nanoparticles, attention should be paid to the specific mechanisms of action at different stages of oral biofilm formation, as well as relevant influencing factors, in order to achieve an objective and comprehensive evaluation. This review is intended to detail the antibacterial mechanisms of nanoparticles during the four stages of the formation of oral biofilms: 1) acquired film formation; 2) bacterial adhesion; 3) early biofilm development; and 4) biofilm maturation. In addition, factors influencing the antibiofilm properties of nanoparticles are summarized from the aspects of nanoparticles themselves, biofilm models, and host factors. The limitations of current research and possible trends for future research are also discussed. In summary, nanoparticles are a promising antioral biofilm strategy. It is hoped that this review can serve as a reference and inspire ideas for further research on the application of nanoparticles for effectively targeting and treating oral biofilms.

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How do wettability, zeta potential and hydroxylation degree affect the biological response of biomaterials?

Cited by 121 publications

References 61 publications

Influence of the wettability of different titanium surface topographies on initial cellular behavior

Influence of the wettability of different titanium surface topographies on initial cellular behavior

Zeta Potential Measurements on Solid Surfaces for in Vitro Biomaterials Testing: Surface Charge, Reactivity Upon Contact With Fluids and Protein Absorption

Nanoparticles for the Treatment of Oral Biofilms: Current State, Mechanisms, Influencing Factors, and Prospects

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