How do meiotic chromosomes meet their homologous partners?: lessons from fission yeast

Yamamoto, Ayumu; Hiraoka, Yasushi

doi:10.1002/bies.1072

Cited by 72 publications

(59 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Loidl and Scherthan (2004) propose that a prolonged bouquet conformation, together with extreme nuclear elongation, replaces SC formation for the initial apposition of homologs along their length in Tetrahymena. Furthermore, they suggest that in S. pombe a prolonged bouquet state, together with moderate nuclear elongation and nuclear movements, might fulfill a similar role to that in Tetrahymena (reviewed by Yamamoto and Hiraoka 2001). However, in the organisms considered above, except Arabidopsis, SC formation was not important for fulllength homolog alignment, even though the meiotic nucleus did not elongate.…”

Section: Species Without Tfs/scsmentioning

confidence: 98%

The diverse roles of transverse filaments of synaptonemal complexes in meiosis

Boer

Heyting

2006

Chromosoma

View full text Add to dashboard Cite

In most eukaryotes, homologous chromosomes (homologs) are closely apposed during the prophase of the first meiotic division by a ladderlike proteinaceous structure, the synaptonemal complex (SC) [Fawcett, J Biophys Biochem Cytol 2:403-406, 1956; Moses, J Biophys Biochem Cytol 2: [215][216][217][218] 1956]. SCs consist of two proteinaceous axes, which each support the two sister chromatids of one homolog, and numerous transverse filaments (TFs), which connect the two axes. Organisms that assemble SCs perform meiotic recombination in the context of these structures. Although much information has accumulated about the composition of SCs and the pathways of meiotic crossing over, several questions remain about the role of SCs in meiosis, in particular, about the role of the TFs. In this review, we focus on possible role(s) of TFs. The interest in TF functions received new impulses from the recent characterization of TF-deficient mutants in a number of species. Intriguingly, the phenotypes of these mutants are very different, and a variety of TF functions appear to be hidden behind a façade of morphological conservation. However, in all TFdeficient mutants a specific class of crossovers that display interference is affected. TFs appear to create suitable preconditions for the formation of these crossovers in most species, but are most likely not directly involved in the interference process itself. Furthermore, TFs are important for full-length homolog alignment. (Fig. 1). Assembly and composition of synaptonemal complexesCohesins, which are proteins that mediate cohesion between sister chromatids (reviewed by Nasmyth 2005), are important for AE assembly (Klein et al. 1999;Pelttari et al. 2001). During S-phase, both in the mitotic cycle and in premeiotic S-phase, cohesins are installed in such a way that they hold the newly synthesized sister chromatids together. In the mitotic cycle, all sister chromatid cohesion is lost at the metaphase-to-anaphase transition, so that sister chromatids can disjoin. In meiosis, two chromosome segregations follow a single round of DNA-replication, and this is possible because cohesion is released in two steps (reviewed by Nasmyth 2001Nasmyth , 2005.Cohesins not only provide sister chromatid cohesion, but also participate in homologous recombination, both in the mitotic cycle and in meiosis. In mitosis, they enhance sister chromatid-based recombinational repair. In meiosis, their role is modified in such a way that homologous recombination occurs preferentially between chromatids of homologs, in most species, mainly or exclusively by a TF-dependent pathway of crossing over (reviewed by

show abstract

Section: Species Without Tfs/scsmentioning

confidence: 98%

The diverse roles of transverse filaments of synaptonemal complexes in meiosis

Boer

Heyting

2006

Chromosoma

View full text Add to dashboard Cite

show abstract

“…This clustering of telomeres is thought to insure proper homolog pairing and synapsis of the homolog axes [60][61][62]. Interestingly both ATM [63] and more recently H2AX [29] have been shown to regulate the persistence of the telomere bouquet.…”

Section: Role Of H2ax In Meiosismentioning

confidence: 99%

H2AX: the histone guardian of the genome

et al. 2004

View full text Add to dashboard Cite

At close hand to one's genomic material are the histones that make up the nucleosome. Standing guard, one variant stays hidden doubling as one of the core histones. But, thanks to its prime positioning, a variation in the tail of H2AX enables rapid modification of the histone code in response to DNA damage. A role for H2AX phosphorylation has been demonstrated in DNA repair, cell cycle checkpoints, regulated gene recombination events, and tumor suppression. In this review, we summarize what we have learned about this marker of DNA breaks, and highlight some of the questions that remain to be elucidated about the physiological role of H2AX. We also suggest a model in which chromatin restructuring mediated by H2AX phosphorylation serves to concentrate DNA repair/signaling factors and/or tether DNA ends together, which could explain the pleotropic phenotypes observed in its absence.

show abstract

“…Soon after the induction of meiosis, centromeres disperse from the SPB (Hayashi et al, 1999;Jin et al, 2000) and telomeres redistribute to cluster transiently at this location (Trelles-Sticken et al, 2000). Thus, the bouquet stage of budding yeast resembles the classical bouquet arrangement that occurs in a conserved manner during prophase I of most, if not all, eukaryotes (Zickler and Kleckner, 1998;Yamamoto and Hiraoka, 2001;Scherthan, 2001).…”

Section: Introductionmentioning

confidence: 99%

Increased ploidy and KAR3 and SIR3 disruption alter the dynamics of meiotic chromosomes and telomeres

Trelles-Sticken

Loidl

Scherthan

2003

Journal of Cell Science

View full text Add to dashboard Cite

We investigated the sequence of chromosomal events during meiotic prophase in haploid, diploid and autotetraploid SK1 strains of Saccharomyces cerevisiae. Using molecular cytology, we found that meiosis-specific nuclear topology (i.e. dissolution of centromere clustering, bouquet formation and meiotic divisions) are significantly delayed in polyploid SK1 meiosis. Thus, and in contrast to the situation in plants, an increase in ploidy extends prophase I in budding yeast. Moreover, we found that bouquet formation also occurs in haploid and diploid SK1 meiosis deficient in the telomeric heterochromatin protein Sir3p. Diploid sir3Δ SK1 meiosis showed pleiotropic defects such as delayed centromere cluster resolution in a proportion of cells and impeded downstream events (i.e. bouquet formation,homologue pairing and meiotic divisions). Meiotic telomere clustering occurred in diploid and haploid sir3Δ strains. Using the haploid system,we further show that a bouquet forms at the kar3Δ SPB. Comparison of the expression of meiosis-specific Ndj1p-HA and Zip1p in haploid control and kar3Δ time courses revealed that fewer cells enter the meiotic cycle in absence of Kar3p. Elevated frequencies of bouquets in kar3Δ haploid meiosis suggest a role for Kar3p in regulation of telomere dynamics.

show abstract

How do meiotic chromosomes meet their homologous partners?: lessons from fission yeast

Cited by 72 publications

References 59 publications

The diverse roles of transverse filaments of synaptonemal complexes in meiosis

The diverse roles of transverse filaments of synaptonemal complexes in meiosis

H2AX: the histone guardian of the genome

Increased ploidy and KAR3 and SIR3 disruption alter the dynamics of meiotic chromosomes and telomeres

Contact Info

Product

Resources

About