How do Highly Branched (Colonial) Mutants of Fusarium Graminearum A3/5 arise during Quorn® Myco-Protein Fermentations?

Abstract

Furthermore, the margins of heterokaryotic colonies generally contained nuclei from only one of the parental homokaryons, indicating imperfect nuclear mixing within the mycelium. These observations suggest that recessive, colonial mutants may appear during Quoma myco-protein production following shear-induced separation of hyphal fragments which contain a sufficiently high ratio of colonial : wild-type nuclei for the colonial phenotype to be expressed.

Identification and complementation of abnormal hyphal branch mutants ahbA1 and ahbB1 in Aspergillus nidulans

“…Decreased branching is a rarely reported as a mutant phenotype in filamentous fungi (Watters and Griffiths, 2001), though increased branching mutants are frequently identified (Bocking et al, 1999;Lauter et al, 1998;Propheta et al, 2001;Sone and Griffiths, 1999;Steele and Trinci, 1977;Wiebe et al, 1996), In the present work we describe the identification and characterization of two abnormal hyphal branch mutants: the hypobranching ahbA1 and hyperbranching ahbB1.…”

Heterokaryosis and Vegetative Incompatibility in Stagonospora nodorum

“…For several complementary nit-based heterokaryons of Fusarium graminearum, even a strong selective pressure was insufficient to maintain heterokaryons in a 'balanced' condition (Wiebe et al, 1996). In Colletotrichum gloeosporioides f. sp.…”

Evolution of a recombinant (gucoamylase‐producing) strain of Fusarium venenatum A3/5 in chemostat culture

“…Wiebe et al (1992aWiebe et al ( , 1996b have previously shown that when heterokaryons are formed between highly branched mutants in this manner, the macroconidia (monokaryotic) formed by the heterokaryon will have the morphology of the two highly branched mutants from which the heterokaryon was formed. All crosses were made on modified Vogel's medium containing only glutamine as the nitrogen source (Wiebe et al, 1992a).…”