How do chromosomes attach to synaptonemal complexes?

Spangenberg, Victor; Dadashev, S. Ya.; Matveevsky, Sergey; Коломиец, О. Л.; Bogdanov, Yu. F.

doi:10.1134/s1022795410100157

Cited by 5 publications

(4 citation statements)

References 5 publications

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“…28 Another possible hypothesis is that H3K27me3 clusters may be involved in the formation of the SC itself. 29 Our findings suggest a model for spatial distribution of chromatin at pachytene stage of meiosis prophase I (Fig. 6).…”

Section: /18 3 Discussionsupporting

confidence: 52%

Super-resolution imaging reveals structurally distinct periodic patterns of chromatin along pachytene chromosomes

Prakash

Fournier

Redl

et al. 2015

Preprint

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During meiosis, homologous chromosomes associate to form a unique structure called synaptonemal complex (SC) whose disruption leads to infertility. Information about the epigenetic features of chromatin within this structure at the level of super-resolution microscopy is largely lacking. We combined single molecule localization microscopy with quantitative analytical methods to describe the epigenetic landscape of meiotic chromosomes at the pachytene stage in mouse oocytes. DNA is found to be nonrandomly distributed along the length of the SC in condensed clusters. Periodic clusters of repressive chromatin (trimethylation of histone H3 at lysine 27, H3K27me3) are found at 500 nm intervals along the SC, while one of the ends of SC displays a large and dense cluster of centromeric histone mark (trimethylation of histone H3 at lysine 9, H3K9me3). Chromatin associated with active transcription (trimethylation of histone H3 at lysine 4, H3K4me3) is arranged in a radial hair-like loop pattern emerging laterally from the SC. These loops seem to be punctuated with small clusters of H3K4me3 mark with an average spread larger than their spacing. Our findings indicate that the nanoscale structure of the pachytene chromosomes is constrained by periodic patterns of chromatin marks, whose function in recombination and higher-order genome organisation is yet to be elucidated.

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Section: /18 3 Discussionsupporting

confidence: 52%

Super-resolution imaging reveals structurally distinct periodic patterns of chromatin along pachytene chromosomes

Prakash

Fournier

Redl

et al. 2015

Preprint

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“…The composition and various types of epigenetic modifications of chromatin play important roles in the synapsis (Spangenberg et al, 2010). Immunodetection of H3K27m3 in T. silvestris revealed a correlation of this epigenetic mark with synapsis during prophase I (Fig.…”

Section: Discussionmentioning

confidence: 99%

Meiosis in the scorpion Tityus silvestris: new insights into achiasmatic chromosomes

et al. 2019

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Achiasmatic male meiosis in scorpions is characterized by a high frequency of gaps, asynaptic regions and multivalent associations. Here, we performed an immunocytogenetic analysis to investigate recombination, and synapsis and chromatin-remodeling events during meiosis of the scorpion Tityus silvestris . Our results demonstrate that the synaptonemal complex (SC) begins its organization in the zygotene stage and persists until metaphase I. The advancement of the synaptic process is related to the epigenetic modification histone H3 lysine 27 trimethylation (H3K27m3). The distribution and dynamics patterns of variant γH2AX and recombinase Rad51 during achiasmatic meiosis suggests formation and repair of DNA double-strand breaks (DSBs) during early stages of prophase I. The epigenetic modifications, histone H3 lysine 4 trimethylation (H3K4m3) and histone H3 lysine 9 acetylation (H3K9ac), showed a dispersed distribution along the bivalents, suggesting that transcriptional activity is maintained constitutively during prophase I. However, H3K9ac modifications are absent in constitutive heterochromatin carrying the 45S rDNA in pachytene and post-pachytene stages. Collectively, our data demonstrate that T. silvestris exhibits adaptations to the achiasmatic mode, and suggest that epigenetic modifications may act in the regulation of these mechanisms to favor the normal continuation of meiosis in this scorpion.

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“…These clusters may be selected to escape recombination, similar to how DNA methylation was suggested to prevent recombination in transposon sequences (34). Another possible hypothesis is that H3K27me3 clusters may be involved in the formation of the SC itself (35).…”

Section: Discussionmentioning

confidence: 99%

Superresolution imaging reveals structurally distinct periodic patterns of chromatin along pachytene chromosomes

Prakash

Fournier

Redl

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

During meiosis, homologous chromosomes associate to form the synaptonemal complex (SC), a structure essential for fertility. Information about the epigenetic features of chromatin within this structure at the level of superresolution microscopy is largely lacking. We combined single-molecule localization microscopy (SMLM) with quantitative analytical methods to describe the epigenetic landscape of meiotic chromosomes at the pachytene stage in mouse oocytes. DNA is found to be nonrandomly distributed along the length of the SC in condensed clusters. Periodic clusters of repressive chromatin [trimethylation of histone H3 at lysine (Lys) 27 (H3K27me3)] are found at 500-nm intervals along the SC, whereas one of the ends of the SC displays a large and dense cluster of centromeric histone mark [trimethylation of histone H3 at Lys 9 (H3K9me3)]. Chromatin associated with active transcription [trimethylation of histone H3 at Lys 4 (H3K4me3)] is arranged in a radial hair-like loop pattern emerging laterally from the SC. These loops seem to be punctuated with small clusters of H3K4me3 with an average spread larger than their periodicity. Our findings indicate that the nanoscale structure of the pachytene chromosomes is constrained by periodic patterns of chromatin marks, whose function in recombination and higher order genome organization is yet to be elucidated.

show abstract

How do chromosomes attach to synaptonemal complexes?

Cited by 5 publications

References 5 publications

Super-resolution imaging reveals structurally distinct periodic patterns of chromatin along pachytene chromosomes

Super-resolution imaging reveals structurally distinct periodic patterns of chromatin along pachytene chromosomes

Meiosis in the scorpion Tityus silvestris: new insights into achiasmatic chromosomes

Superresolution imaging reveals structurally distinct periodic patterns of chromatin along pachytene chromosomes

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