Background: Gambling advertising employs a range of persuasive strategies, yet few interventions have been established to foster resilience against the commercial tactics of the gambling industry. There is a consequent need for these ‘counter-advertising’ interventions that are developed independently from the gambling industry, and that incorporate the input of experts with lived experience of gambling-related harm (Experts-by-Experience; EbyE). Aims: We therefore aimed to evaluate the feasibility and acceptability of a counter-advertising intervention video to increase resilience to gambling advertising persuasion. Methods: Three in-depth focus groups were conducted, and each group contained a mixture of gambling-related academics (N = 12) and EbyEs (N = 10). Participants were given access to the intervention video, and subsequently provided their perceptions and feedback during the focus groups. Qualitative data were audio recorded, transcribed, and thematically analysed by the research team via an inductive approach. Results: Three main themes were identified. Firstly, participants recommended a shorter video that had a simplified and digestible structure. Second, frequent real-world examples of gambling advertisements within the video were discouraged, and the inclusion of a relatable human voiceover was considered imperative to the receptiveness of the video. Finally, participants deemed it important to deliver psychologically grounded yet jargon-free content via a conversational style. An overall narrative framed by consumer-protection was also preferred in order to increase acceptance of the video content, rather than a more didactic framing. Conclusions: Evaluating the acceptability of a counter advertising intervention video provided valuable insight from both an academic and lived-experience perspective. Such insight is instrumental to the meaningful co-design of counter-advertising interventions.