Abstract:Control of protein adsorption is essential for successful integration of healthcare materials into the body. Human plasma fibrinogen (HPF), especially its conformation is a key upstream regulator for platelet behavior and thus pathological clot formation at the blood‐biomaterial interface. A previous study by the authors revealed that the conformation of adsorbed HPF can be controlled by rutile surface crystallographic orientation. Therefore, it is hypothesized that pre‐adsorbed HPF on specific rutile orientat… Show more
“…The antifouling performance of the polymer brushes was further assessed against Fib, which is known to play an important role in mediating the interactions between implants and blood . Adsorption on the implant surface of a very small amount of Fib can induce subsequent adhesion and coagulation of a large quantity of platelets, causing failure after implantation. , Figure compares the adsorption of Fib on different surfaces observed by QCM-D. It can be seen that onto a bare gold substrate, Fib adsorbs in a much greater quantity than BSA (Figure a) even from a solution of a much lower concentration of 0.1 mg/mL, showing its higher affinity to the surface.…”
Antifouling surfaces have attracted increasing interest
in recent
years due to their potential application in various fields. In this
work, we report a loop polyzwitterionic coating that exhibits excellent
resistance to protein adsorption. Triblock and diblock copolymers
of 2-[(2-hydroxyethyl)disulfanyl]ethyl methacrylate) (HSEMA) and 2-(dimethylamino)ethyl
methacrylate) (DMAEMA) were synthesized by atom-transferred radical
polymerization, followed by betainization of the DMAEMA block with
1,3-propane sultone and reduction of the disulfide bond in HSEMA to
yield a triblock copolymer comprising a zwitterionic poly(sulfobetaine
methacrylate) (PSBMA) midblock and poly(2-sulfanylethyl methacrylate)
(PSEMA) terminal blocks as well as its diblock analogue that was of
the same composition as the former and half the chain length. Both
copolymers adsorbed to the gold substrate via the thiol groups in
the terminal PSEMA block(s), creating loop and linear PSBMA brush
coatings of comparable thickness, as revealed by X-ray photoelectron
spectroscopy and ellipsometry. Adsorption of bovine serum albumin
and fibrinogen as model proteins from solution to these surfaces was
investigated by a quartz crystal microbalance with dissipation and
confocal laser scanning microscopy (CLSM), and platelet and bacterial
adhesions were assessed by scanning electron microscopy and CLSM.
The results demonstrate that both linear and loop polyzwitterion brushes
are excellent in resisting the adsorption of the foulants, and the
loop brushes are superior to the linear analogues.
“…The antifouling performance of the polymer brushes was further assessed against Fib, which is known to play an important role in mediating the interactions between implants and blood . Adsorption on the implant surface of a very small amount of Fib can induce subsequent adhesion and coagulation of a large quantity of platelets, causing failure after implantation. , Figure compares the adsorption of Fib on different surfaces observed by QCM-D. It can be seen that onto a bare gold substrate, Fib adsorbs in a much greater quantity than BSA (Figure a) even from a solution of a much lower concentration of 0.1 mg/mL, showing its higher affinity to the surface.…”
Antifouling surfaces have attracted increasing interest
in recent
years due to their potential application in various fields. In this
work, we report a loop polyzwitterionic coating that exhibits excellent
resistance to protein adsorption. Triblock and diblock copolymers
of 2-[(2-hydroxyethyl)disulfanyl]ethyl methacrylate) (HSEMA) and 2-(dimethylamino)ethyl
methacrylate) (DMAEMA) were synthesized by atom-transferred radical
polymerization, followed by betainization of the DMAEMA block with
1,3-propane sultone and reduction of the disulfide bond in HSEMA to
yield a triblock copolymer comprising a zwitterionic poly(sulfobetaine
methacrylate) (PSBMA) midblock and poly(2-sulfanylethyl methacrylate)
(PSEMA) terminal blocks as well as its diblock analogue that was of
the same composition as the former and half the chain length. Both
copolymers adsorbed to the gold substrate via the thiol groups in
the terminal PSEMA block(s), creating loop and linear PSBMA brush
coatings of comparable thickness, as revealed by X-ray photoelectron
spectroscopy and ellipsometry. Adsorption of bovine serum albumin
and fibrinogen as model proteins from solution to these surfaces was
investigated by a quartz crystal microbalance with dissipation and
confocal laser scanning microscopy (CLSM), and platelet and bacterial
adhesions were assessed by scanning electron microscopy and CLSM.
The results demonstrate that both linear and loop polyzwitterion brushes
are excellent in resisting the adsorption of the foulants, and the
loop brushes are superior to the linear analogues.
“…It is well known that the insertion of, for example, a temporary central venous or peripheral arterial catheter leads to the formation of blood clots on the inner wall of the catheter. It is also well known that platelets are responsible for this in the context of primary hemostasis, which spontaneously adhere to the foreign surface mediated by NSA of mainly Fg ( Struczyńska et al, 2023 ), but also Fn( Lin et al, 2019 ) and other glycoproteins. ( Ward, 2008 ).…”
The fouling resistance of zwitterionic coatings is conventionally explained by the strong hydrophilicity of such polymers. Here, the in vitro biocompatibility of a set of systematically varied amphiphilic, zwitterionic copolymers is investigated. Photocrosslinkable, amphiphilic copolymers containing hydrophilic sulfobetaine methacrylate (SPe) and butyl methacrylate (BMA) were systematically synthesized in different ratios (50:50, 70:30, and 90:10) with a fixed content of photo-crosslinker by free radical copolymerization. The copolymers were spin-coated onto substrates and subsequently photocured by UV irradiation. Pure pBMA and pSPe as well as the prepared amphiphilic copolymers showed BMA content-dependent wettability in the dry state, but overall hydrophilic properties a fortiori in aqueous conditions. All polysulfobetaine-containing copolymers showed high resistance against non-specific adsorption (NSA) of proteins, platelet adhesion, thrombocyte activation, and bacterial accumulation. In some cases, the amphiphilic coatings even outperformed the purely hydrophilic pSPe coatings.
“…Such conformational changes result in altered platelet adhesion in the two phases. As seen in Figure 3, the hydrophilic (001) phase has a higher distribution of platelets, and therefore presents a higher risk of thrombogenicity [9]. Figure 3 Platelet adhesion on the different phases after 120 minutes under static conditions.…”
mentioning
confidence: 99%
“… Figure 3 Platelet adhesion on the different phases after 120 minutes under static conditions. Image reproduced without modification from [9]. …”
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