How Cell Number and Cellular Properties of Blood-Banked Red Blood Cells of Different Cell Ages Decline during Storage

Tuo, Wei-Wei; Wang, Di; Liang, Wenjing; Huang, Yao‐Xiong

doi:10.1371/journal.pone.0105692

Cited by 22 publications

(31 citation statements)

References 49 publications

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“…Age-and sex-associated effects on the average RBC life span may address in part the large heterogeneity observed in the mean age of RBCs among eligible blood donors [52]. Although stored RBCs degrade over time through more than one mechanisms of cellular injury [36], including disturbance in redox and metabolism homeostasis [1], RBCs' age at the time of donation probably affects the quality of stored RBC concentrates [53,117]. In terms of posttransfusion efficacy, the effectiveness of transfused young RBCs ("neocytes") seems to be less than predicted by early in vitro and in vivo studies [118,119] and varies among patients [120] probably because neocytes are more sensitive to the presence or absence of survival signals compared to older cells [121].…”

Section: Nongenetic Factors May Contribute To the Donor-variation Effmentioning

confidence: 99%

Donor‐variation effect on red blood cell storage lesion: A close relationship emerges

Tzounakas¹,

Kriebardis

Papassideri³

et al. 2016

Proteomics Clinical Apps

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Although the molecular pathways leading to the progressive deterioration of stored red blood cells (RBC storage lesion) and the clinical relevance of storage-induced changes remain uncertain, substantial donor-specific variability in RBC performance during storage, and posttransfusion has been established ("donor-variation effect"). In-bag hemolysis and numerous properties of the RBC units that may affect transfusion efficacy have proved to be strongly donor-specific. Donor-variation effect may lead to the production of highly unequal blood labile products even when similar storage strategy and duration are applied. Genetic, undiagnosed/subclinical medical conditions and lifestyle factors that affect RBC characteristics at baseline, including RBC lifespan, energy metabolism, and sensitivity to oxidative stress, are all likely to influence the storage capacity of individual donors' cells, although not evident by the donor's health or hematological status at blood donation. Consequently, baseline characteristics of the donors, such as membrane peroxiredoxin-2 and serum uric acid concentration, have been proposed as candidate biomarkers of storage quality. This review article focuses on specific factors that might contribute to the donor-variation effect and emphasizes the emerging need for using omics-based technologies in association with in vitro and in vivo transfusion models and clinical trials to discover biomarkers of storage quality and posttransfusion recovery in donor blood.

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Section: Nongenetic Factors May Contribute To the Donor-variation Effmentioning

confidence: 99%

Donor‐variation effect on red blood cell storage lesion: A close relationship emerges

Tzounakas¹,

Kriebardis

Papassideri³

et al. 2016

Proteomics Clinical Apps

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“…The aging-produced 'senescent' cells are characterized by the loss of cell surface area and cell morphology alterations [7], resulting from the shedding of hemoglobin(Hb)-containing vesicles [3,6,8,9]. The cold storage of packed RBCs (PRBCs) is associated with a continuous increase in the percentage of cells with impaired functionality [10][11][12]. This included increased cell rigidity (reduced deformability) and aggregability [12][13][14], reduced level of cell membrane stomatin [15], and translocation of phosphatidylserine (PS) to the cell surface [9,16], all leading to an increased adherence to endothelial cells [14].…”

Section: Introductionmentioning

confidence: 99%

Biophysical and Biochemical Markers of Red Blood Cell Fragility

Orbach¹,

Zelig

Yedgar³

et al. 2017

Transfus Med Hemother

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Background: Red blood cells (RBCs) undergo a natural aging process occurring in the blood circulation throughout the RBC lifespan or during routine cold storage in the blood bank. The aging of RBCs is associated with the elevation of mechanical fragility (MF) or osmotic fragility (OF) of RBCs, which can lead to cell lysis. The present study was undertaken to identify RBC properties that characterize their susceptibility to destruction under osmotic/mechanical stress. Methods: RBCs were isolated from freshly donated blood or units of packed RBCs (PRBCs) and suspended in albumin-supplemented phosphate-buffered saline (PBS). In addition, PRBCs were separated by filtration through a microsphere column into two fractions: enriched with rigid (R-fraction) and deformable (D-fraction) cells. The RBCs were subjected to determination of deformability, MF and OF, moreover, the level of cell surface phosphatidylserine (PS) and the stomatin level in isolated RBC membranes were measured. Results: In the RBC population, the cells that were susceptible to mechanical and osmotic stress were characterized by low deformability and increased level of surface PS. The OF/MF was higher in the R-fraction than in the D-fraction. Stomatin was depleted in destroyed cells and in the R-fraction. Conclusion: RBC deformability, the levels of surface PS, and membrane stomatin can be used as markers of RBC fragility.

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“…The amount of membrane SA in an old RBC (aged about 90–120 days) is approximately 30% less than that of a young cell(aged 1–30 days) . The deformability of the cell membrane, the oxygen‐carrying capacity, and the amounts of Na + , K + ‐ATPase, Ca ++ Mg ++ ‐ATPase, and 2,3‐diphosphoglycerate (2,3‐DPG) also decrease concurrently . Red blood cells unfit for circulation are recognized and phagocytized by the reticuloendothelial system.…”

Section: Introductionmentioning

confidence: 99%

“…The mean survival time of old RBCs is usually not more than 30 days . In a freshly drawn RBC unit, old RBCs comprise ~16% of the total RBCs .…”

Section: Introductionmentioning

confidence: 99%

Restoring the youth of aged red blood cells and extending their lifespan in circulation by remodelling membrane sialic acid

Huang¹,

Tuo²,

Wang³

et al. 2015

J Cellular Molecular Medi

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Membrane sialic acid (SA) plays an important role in the survival of red blood cells (RBCs), the age‐related reduction in SA content negatively impacts both the structure and function of these cells. We have therefore suggested that remodelling the SA in the membrane of aged cells would help recover cellular functions characteristic of young RBCs. We developed an effective method for the re‐sialylation of aged RBCs by which the cells were incubated with SA in the presence of cytidine triphosphate (CTP) and α‐2,3‐sialytransferase. We found that RBCs could be re‐sialylated if they had available SA‐binding groups and after the re‐sialylation, aged RBCs could restore their membrane SA to the level in young RBCs. Once the membrane SA was restored, the aged RBCs showed recovery of their biophysical and biochemical properties to similar levels as in young RBCs. Their life span in circulation was also extended to twofold. Our findings indicate that remodelling membrane SA not only helps restore the youth of aged RBCs, but also helps recover injured RBCs.

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How Cell Number and Cellular Properties of Blood-Banked Red Blood Cells of Different Cell Ages Decline during Storage

Cited by 22 publications

References 49 publications

Donor‐variation effect on red blood cell storage lesion: A close relationship emerges

Donor‐variation effect on red blood cell storage lesion: A close relationship emerges

Biophysical and Biochemical Markers of Red Blood Cell Fragility

Restoring the youth of aged red blood cells and extending their lifespan in circulation by remodelling membrane sialic acid

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