How biophysical <i>in vivo</i> testing techniques can be used to characterize full thickness skin equivalents

Houcine, Audrey; Delalleau, Alexandre; Heraud, Sandrine; Guiraud, B.; Payré, Bruno; Duplan, Hélène; Delisle, Marie–Bernadette; Damour, O.; Bessou‐Touya, S.

doi:10.1111/srt.12259

Cited by 9 publications

(9 citation statements)

References 42 publications

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“…Of course, the SC of the calf and of the dorsal forearm is significantly thicker than that of the volar forearm, abdomen, and back. The thickness of the SC of the calf region could be due to less exfoliation contrary to other body sites [17]. A thicker SC on the dorsal forearm, as a UV-expressed skin area compared to nonchronically sun-exposed skin areas – such as the abdomen, volar forearm, and the back – goes hand in hand with the results found by Huzaira et al [29], who detected, by means of CRS, a thinner and rougher SC in sun-protected skin areas compared to skin areas exposed to the sun.…”

Section: Discussionmentioning

confidence: 99%

“…The gluteal region is a good example of a skin location exposed to high mechanical stress. With greater strain on the skin, the elastic characteristics of the tissue increase [17]. …”

Section: Discussionmentioning

confidence: 99%

“…OCT can be employed as a comparative reference to 2PM and can verify the quality and applicability of 2PM [4, 19]. The depiction of the SC and the exact determination of its thickness has not been possible up to now, either invasively or noninvasively [3, 17]. Quantification by OCT may be possible only at the palm and plantar and with hyperkeratotic skin (SC > 50 µm) [4, 6].…”

Section: Introductionmentioning

confidence: 99%

“…LSM and CRS produce horizontal images in multiple stacks, whereas OCT generates vertical images of a cross section of both the epidermis and dermis. Thus, the latter method may establish a direct comparison to the histological findings in order to evaluate the ET and morphological structures [6, 12, 14, 17, 18]. …”

Section: Introductionmentioning

confidence: 99%

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Noninvasive Determination of Epidermal and Stratum Corneum Thickness in vivo Using Two-Photon Microscopy and Optical Coherence Tomography: Impact of Body Area, Age, and Gender

Czekalla

Schönborn

Lademann

et al. 2019

Skin Pharmacol Physiol

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Background: High-resolution images of the epidermis are important to understand the transdermal penetration and changes in epidermal components. Both ex vivo and in vivo technologies are available to picture the epidermal thickness (ET). So far, the illustration of the stratum corneum (SC) has not been possible without artifacts. Objective: Precision in vivo measurement of the ET and SC, duly considering the impact of location on the body, age, and gender. Methods: In this pilot study on 20 skin-healthy subjects aged 18–66 years, the ET was imaged by two-photon microscopy (2PM) and optical coherence tomography (OCT), and the SC by using 2PM at five different body sites. Results: On solar-exposed body areas, both the epidermis and SC are thicker compared to solar-protected areas (p < 0.05), the epidermis at the gluteal region being the thickest (p < 0.05). The ET decreases with age (p < 0.05). Males show a thicker epidermis than females (p < 0.05). Conclusion: 2PM provides a noninvasive method for imaging the epidermis and especially the SC in vivo and is optimally suited for the application of histological criteria.

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Section: Discussionmentioning

confidence: 99%

“…The gluteal region is a good example of a skin location exposed to high mechanical stress. With greater strain on the skin, the elastic characteristics of the tissue increase [17]. …”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Noninvasive Determination of Epidermal and Stratum Corneum Thickness in vivo Using Two-Photon Microscopy and Optical Coherence Tomography: Impact of Body Area, Age, and Gender

Czekalla

Schönborn

Lademann

et al. 2019

Skin Pharmacol Physiol

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“…We first reconstituted a full‐thickness skin model using previously reported engineering techniques . Briefly, a dermal equivalent was prepared by seeding fibroblasts on top of a scaffold substrate made of chitosan‐crosslinked collagen‐glycosaminoglycan matrix.…”

Section: Section 3: In Vitro Clinical and In‐daily Clinical Practicementioning

confidence: 99%

Gerontodermatology: the fragility of the epidermis in older adults

Katoh

Tennstedt

Kan³

et al. 2018

Acad Dermatol Venereol

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The proportion of adults over 60 years of age is rapidly increasing and is estimated to reach approximately one-sixth of the global population by 2030. An ageing population is a real challenge for healthcare resources, including dermatologists and geriatricians, as age-related changes in skin integrity and barrier function make older adults more susceptible to developing skin pathologies such as pruritus, dermatitis and infections. Fragile skin arises from several interlinked causes, including age-related changes in skin barrier integrity, previous and current lifestyle choices, skin pathologies and medical interventions. Dermo-cosmetics can play a key role in enhancing skin care regimens and preventing pathologies in this age group. In vitro studies, clinical, and in-daily clinical practice studies of dermo-cosmetics have shown them to be effective in many skin conditions in older adults, like xerosis and pruritus. Incontinence-associated dermatitis (IAD), a common condition arising from contact with irritants such as urine and faeces which can significantly impact the quality of life of sufferers, can also be improved with a barrier cream in incontinent patients aged 70 years and older. This supplement focuses on the increased fragility of older skin, the development of common skin pathologies and the efficacy and tolerance of dermo-cosmetic products in older adults.JEADV alcohol consumption and sun exposure), medical procedures (e.g. surgery) and underlying pathologies can all contribute to the development of fragile skin. While not necessarily associated with disease per se, most patients presenting to dermatologists with fragile skin have pathological conditions such as acne, psoriasis and atopic dermatitis. 4 However, surveys reveal that a substantial proportion of people have perceived fragile skin, which, while not associated with disease, can cause redness, tightness, dryness and itching. 5,6 Globally, the incidence of fragile skin in adults has been estimated as 25-52%, across skin types and age groups. [4][5][6] In older adults, skin ageing makes individuals more vulnerable to develop fragile skin and associated pathologies resulting from impaired skin barrier function. The mechanisms and clinical relevance of age-related skin deterioration, the prevalence of skin disorders in older adults in Europe and recent in vitro, clinical and in-daily clinical practice studies of fragile skin in this age group will be covered in this supplement. Section 1: Fragile skin in older adultsIn addition to internal age-related changes in skin structure and functionality, physiological, circumstantial, pathological and iatrogenic fragile skin causes also contribute to the weakening of the epidermis and skin barrier in older adults. Physiological fragile skinOlder adults experience physiological fragile skin, as declines in the immune system function within the epidermis, and changes in epidermal and dermal structure contribute to a reduction in the effectiveness of the skin barrier function. 7 During adulthood, the skin ...

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