How an ACE2 mimicking epitope-MIP nanofilm recognizes template-related peptides and the receptor binding domain of SARS-CoV-2

Abstract

Here we aim to the mechanistic understanding of the formation of epitope-imprinted polymer nanofilms using non-terminal peptide sequence, i.e. the peptide GFNCYFP (G485 to P491) of SARS-CoV-2 receptor binding domain...

Novel functional monomer for the electrochemical synthesis of highly affine epitope‐imprinted polymers

“…The MIP generation based on this monomer could integrate epitope imprinting, surface imprinting and oriented template immobilization in a microarray format. The latter was achieved by microspotting first the peptide epitopes onto the gold surface of the SPRi biochip, which enabled the adjustment of the optimal GFNCYFPLQ peptide SARS-CoV-2 spike protein RBD SPRi 1.6 [18] GFNCYFP peptide SARS-CoV-2 spike protein RBD SPRi 2.2 [18] GFNCYFP peptide SARS-CoV-2 spike protein RBD SWV 57.6 [43] Cytochrome C Cytochrome C CV 10,000 [31] AYLKKATNE peptide Cytochrome C FL 8,540 [44] Transferrin Transferrin SWV 500 [37] HAS HSA CV 2,800 [19] VHLTP peptide Hemoglobin SWV 9.06 [b] [45]…”

“…The results confirm, at least for this model case, the hypothesis that increased number of functionalities of the monomer may lead to higher affinity MIPs towards the target. The epitope-imprinted poly(Asp-UMB) proved to be superior in terms of its affinity towards RBD as compared with peptide epitope-imprinted polyscopoletin determined in the same conditions [18,43], although did not reach the K D value of 77 fM reported for RBD-imprinted poly(orto-phenylenediamine) based on EIS-based measurements [49]. The affinity is also outstanding when compared with the structurally similar polyscopoletin-based MIPs with K D values in the nanomolar range (Table 1.)…”