How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry

Sabater, S.; Berenguer, R.; Honrubia-Gómez, Paloma; Rivera, Miguel N.; Nuñez, Ana Teresa; Jimenez-Jimenez, E.; Martos, A.; Ramírez-Castillejo, Carmen

doi:10.1093/jrr/rru022

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…When using multiwell tissue culture plates, because of the multiple air-tissue interfaces, there is concern about dose homogeneity between and within wells, which can affect the interpretation of the results and introduce uncertainties in radiobiology experiments. There was up to 4% underdosing at the air-fluid interface in multiwell tissue culture plate wells but this was not seen at the bottom of wells where cells were attached and grown [27] and our results confirm this observation, which may alleviate some of these concerns.…”

Section: Discussionsupporting

confidence: 77%

A High-Throughput In Vitro Radiobiology Platform for Megavoltage Photon Linear Accelerator Studies

et al. 2022

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We designed and developed a multiwell tissue culture plate irradiation setup, and intensity modulated radiotherapy plans were generated for 96-, 24-, and 6-well tissue culture plates. We demonstrated concordance between planned and measured/imaged radiation dose profiles using radiochromic film, a 2D ion chamber array, and an electronic portal-imaging device. Cell viability, clonogenic potential, and g-H2AX foci analyses showed no significant differences between intensity-modulated radiotherapy and open-field, homogeneous irradiations. This novel platform may help to expedite radiobiology experiments within a clinical environment and may be used for wide-ranging ex vivo radiobiology applications.

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Section: Discussionsupporting

confidence: 77%

A High-Throughput In Vitro Radiobiology Platform for Megavoltage Photon Linear Accelerator Studies

et al. 2022

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“…Figure 4 illustrated the PDD curve of OSL dosimeters in comparison to EBT3 film and TPS in in the presence of air cavities of 3, 5 and 8 cm thicknesses. The presence of air cavities significantly reduced the depth dose in all air cavity thicknesses [11]. The depth dose in OSL dosimeters showed good agreement with EBT3 film dosimeters at all air cavity thicknesses.…”

Section: Resultsmentioning

confidence: 57%

Depth dose measurement by using Al₂O₃ OSL dosimeters in high energy photons in the presence of air cavity and density inhomogeneity

Fahmi Mohd Yusof,

Sauki,

Ateqah Suzaini

et al. 2024

IOP Conf. Ser.: Mater. Sci. Eng.

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Air cavities and tissue density inhomogeneity significantly affects the distribution of radiation doses, potentially resulting in adverse consequences such as cancer recurrence. This research aims to assess the accuracy of Al2O3 optically stimulated luminescence (OSL) dosimeters in measuring doses within varying thicknesses of air cavities (3, 5, and 8 cm) and tissue inhomogeneity of low and high density simulated by the lung and bone phantoms. An expanded polystyrene (EPS) was employed in homogeneous solid water® phantoms to simulate the air cavity. The percentage depth-dose (PDD) curves at 6 MV photons were obtained in both presence of air cavity and density inhomogeneity and compared to that in the EBT3 radiochromic film dosimeters and treatment planning system (TPS). The results indicated that the presence of an air cavity and tissue inhomogeneity affected the depth dose measured in OSL dosimeters, EBT3 films and TPS. OSLD and TPS showed good agreement at the centre of the cavity, which is within ±5% but could not estimate scattered radiation to the distal and proximal surfaces of the air cavity. The obtained p-values showed no significant differences of dose measured in OSL dosimeters compared to those in EBT3 films and TPS. The Kruskal Wallis test and Mann-Whitney showed no significant difference between OSL dosimeters, EBT3 film and TPS in the measurement of depth doses in the presence of density inhomogeneity. The overall results indicated the suitability of OSL dosimeters as indirect dosimeters for the measurements of depth dose in the presence of air cavity and tissue density inhomogeneity

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“…Inadequate consideration of buildup and backscatter materials may result in unexpected or undiscovered errors in radiation dose administered to cells in culture plates. The discrepancy between the prescribed and measured dose for adherent cells in a culture plate may be 1.5% to 4%, depending on the setup and irradiation conditions (Kulmala et al 1995, Tomic et al 2007, Sabater et al 2014. Without adequate consideration of the conditions in which cells are exposed, cell viability data may not precisely reflect a response to the stated prescription dose.…”

Section: Introductionmentioning

confidence: 99%

Impact of various irradiation conditions on delivered dose and cell viability for in vitro cell irradiation

Rogers¹,

Lawrence²,

Ehler³

et al. 2019

Biomed. Phys. Eng. Express

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Introduction: To correlate dose from ionizing radiation with cell kill probability, viability and clonogenic assays are often performed following in vitro irradiation of cells in 96-well culture plates. The objective of this work was to examine how dose to adherent cells in 96-well culture plates depended on the conditions in which the irradiations were performed. The aim was to provide support for standardized irradiation setup conditions so that in vitro cell assays can be more precisely compared. Materials and Methods: 96-well culture plates were exposed to a 6 MV photon beam from a linear accelerator. Dose measurements were performed by securing a piece of EBT3 Gafchromic film to the underside of each plate. Each well contained either 200 μl of tap water or canine osteosarcoma cells (OSCA 40) in 200 μl of media. Exposures were performed to assess the dependence of dose to the depth of measurement, the thickness of the backscatter material, the field size, and the position of a plate within the treatment field. Results: Culture plate doses demonstrated a strong dependence on backscatter material thickness and field size. Providing less than 0.5 cm of water equivalent backscatter material resulted in a mean measured underdose of more than 3% for a 30×30 cm 2 field. Decreasing the field size to 13×9 cm 2 yielded a mean measured dose within 0.5% of the prescribed dose. At the central-axis depth where maximum dose occurs (d max =1.5 cm), irradiation of four plates simultaneously resulted in a mean measured dose 3.5% higher than measurements of single plates placed in the center of the field. Conclusions: Inadequate consideration of in vitro irradiation setup conditions is likely to result in discrepancies between prescribed and delivered doses. Such discrepancies can be minimized by providing 5 cm of backscatter material and 10 cm of buildup material; and by exposing one centrally located plate at a time.

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How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry

Cited by 4 publications

References 10 publications

A High-Throughput In Vitro Radiobiology Platform for Megavoltage Photon Linear Accelerator Studies

A High-Throughput In Vitro Radiobiology Platform for Megavoltage Photon Linear Accelerator Studies

Depth dose measurement by using Al₂O₃ OSL dosimeters in high energy photons in the presence of air cavity and density inhomogeneity

Impact of various irradiation conditions on delivered dose and cell viability for in vitro cell irradiation

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How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry

Cited by 4 publications

References 10 publications

A High-Throughput In Vitro Radiobiology Platform for Megavoltage Photon Linear Accelerator Studies

A High-Throughput In Vitro Radiobiology Platform for Megavoltage Photon Linear Accelerator Studies

Depth dose measurement by using Al2O3 OSL dosimeters in high energy photons in the presence of air cavity and density inhomogeneity

Impact of various irradiation conditions on delivered dose and cell viability for in vitro cell irradiation

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Depth dose measurement by using Al₂O₃ OSL dosimeters in high energy photons in the presence of air cavity and density inhomogeneity