Houttuynia cordata Thunb extract modulates G0/G1 arrest and Fas/CD95-mediated death receptor apoptotic cell death in human lung cancer A549 cells

Abstract

BackgroundHouttuynia cordata Thunb (HCT) is commonly used in Taiwan and other Asian countries as an anti-inflammatory, antibacterial and antiviral herbal medicine. In this study, we investigated the anti-human lung cancer activity and growth inhibition mechanisms of HCT in human lung cancer A549 cells.ResultsIn order to investigate effects of HCT on A549 cells, MTT assay was used to evaluate cell viability. Flow cytometry was employed for cell cycle analysis, DAPI staining, and the Comet assay was used for DNA fragmentation and DNA condensation. Western blot analysis was used to analyze cell cycle and apoptotic related protein levels. HCT induced morphological changes including cell shrinkage and rounding. HCT increased the G0/G1 and Sub-G1 cell (apoptosis) populations and HCT increased DNA fragmentation and DNA condensation as revealed by DAPI staining and the Comet assay. HCT induced activation of caspase-8 and caspase-3. Fas/CD95 protein levels were increased in HCT-treated A549 cells. The G0/G1 phase and apoptotic related protein levels of cyclin D1, cyclin A, CDK 4 and CDK 2 were decreased, and p27, caspase-8 and caspase-3 were increased in A549 cells after HCT treatment.ConclusionsThe results demonstrated that HCT-induced G0/G1 phase arrest and Fas/CD95-dependent apoptotic cell death in A549 cells

Carnosic acid induces apoptosis through inactivation of Src/STAT3 signaling pathway in human renal carcinoma Caki cells

“…Since the STAT3-regulated gene products, such as Bcl-xL, Bcl-2, survivin, c-Myc and D-series of cyclins are involved in enhanced cell proliferation, selective inhibition of STAT3 signaling can suppress proliferation and induce apoptosis in cancer cells (19). Previous studies have shown that suppression of STAT3 signaling inhibits the growth of various cancer cells including those of the stomach, liver, head and neck, skin, and lung (22). STAT3, a signal mediator of various pro-inflammatory cytokines and growth factors acting constitutively in an inflammatory tumor microenvironment, is an important molecular target of various anticancer drugs (23).…”

Ethanol extract ofHedyotis diffusawilld upregulates G0/G1 phase arrest and induces apoptosis in human leukemia cells by modulating caspase cascade signaling and altering associated genes expression was assayed by cDNA microarray

“…HL‐60 cells in 12‐well plates were treated with or without 100 μg/mL of EEHDW for 48 h. Cells were thereafter fixed in 4% paraformaldehyde and exposed to 1 μg/mL of 4′,6‐diamidino‐2‐phenylindole (DAPI) (Molecular Probes by Life Technologies, Eugene, OR, USA) staining solution in darkness. The apoptotic cells were observed using fluorescence microscopy (Zeiss, Oberköchen, Germany) as previously described (Huang et al, ; Chen et al, ).…”

Herba houttuyniae Extract Benefits Hyperlipidemic Mice via Activation of the AMPK/PGC-1α/Nrf2 Cascade

“…Although many biological functions have been proposed for Herba houttuyniae [28][29][30][31][32], its effects on MS and hyperlipidemia-associated abnormalities remain largely uncharacterized. The main component of fresh Herba houttuyniae is water, and no major components account for large proportions of the dried weight of Herba houttuyniae [28].…”

“…Houttuynia cordata Thunb, also known as Herba houttuyniae or Houttuyniae herba, is not only a widely distributed medicinal plant but also a functional food with important biological actions, mainly including anti-inflammatory [28], antiviral [29], antioxidant [30], and antitumor [31] effects. Recently, a few studies have shown that Herba houttuyniae may also play a protective role in diabetes because of its anti-inflammatory activity [30,32].…”