House dust mite specific in vitro IgE determination in cats with allergic dermatitis

Abstract

The purpose of this retrospective research was to verify underlying causes of clinical findings in cats with allergic dermatitis. Allergen specific immunolglobulin E (IgE) concentrations against Dermatophagoides farinae and Dermatophagoides pteronyssinus were determined by use of in vitro Polycheck Veterinary allergy tests. Total of 33 cats were referred to clinic with primary/secondary skin lesions or to those of general health status checkup. On initial referral available haematological, microbiological, parasitological and relevant tests were performed. Nineteen cats were deemed healthy based on relevant analysis (neither infection nor hypersensitivity) were enrolled as a control group. Other 14 cats were composed of allergic cases. Regarding allergen specific IgE levels (kU/l) in allergic cats, in vitro tests gave a positive reactions for D. farinae [class 3,4 (n=1), class 2 (n=7), class 1 (n=6) and class 0 (n=0)] and D. pteronyssus [class 3,4 (n=1), class 2(n=5), class 1(n=4) and class 0 (n=4)] in whom at least 1 antigen was detected in all allergic cats. There was a statistical significance for specific IgE levels between healthy controls and allergic cats against house dust mites 0.21±0.018 vs. 4.69±1.49 (kU/l) for D. farinae (p=0.0001) and significant alterations between healthy and allergic cats 0.21±0.023 vs. 3.11±1.21 (kU/l) (p=0.003) for D. pteronyssus. In this study, all positive reactions on the allergy test were suggested to present underlying house dust allergens of dermatitis in enrolled cats. As far as wellknown factor that in vitro allergy tests solely might not be reflecting hypersensitivity from healthy cases because of non-unique clinically findings. Alerjik dermatitli kedilerde ev tozu akarlarına spesifik in vitro Ig E düzeylerinin araştırılması ÖZ Bu retrospektif araştırmanın amacı, alerjik dermatitli kedilerin klinik bulgularının altında yatan nedenleri tespit etmektir. Dermatophagoides farina ve Dermatophagoides pteronyssinus'a karşı alerjen spesifik Ig E konsantrasyonları, in vitro Polycheck Veteriner alerji testleri kullanılarak belirlendi. Primer / sekonder deri lezyonları olan veya genel sağlık durumu kontrolü için gelen toplamda 33 kedi çalışmaya alındı. Öncelikle mevcut hematolojik, mikrobiyolojik, parazitolojik ve ilgili analizler yapıldı. On dokuz kedi, ilgili analize dayalı olarak sağlıklı kabul edildi (ne enfeksiyon ne de aşırı duyarlılık gelişmiş) kontrol grubu olarak kaydedildi. Diğer 14 kedi alerjik vakalardan oluşuyordu. Alerjik kedilerde alerjene spesifik IgE seviyeleri (kU / l) ile ilgili olarak, in vitro testler, D. farinae için [sınıf 3,4 (n = 1), sınıf 2 (n = 7), sınıf 1 (n = 6), sınıf 0 (n= 0)] ve D. pteronyssinus için[sınıf 3,4 (n = 1), sınıf 2 (n = 5), sınıf 1 (n = 4) ve sınıf 0 (n = 4)] pozitif reaksiyonlar verdi. Bütün alerjik kedilerde en az 1 antijen saptandı. Sağlıklı kontrol grubu ve alerjik kediler arasında ev tozu akarlarına karşı spesifik Ig E seviyeleri için istatistiksel olarak anlamlı bir farklılık mevcuttu; D. farinae için 0.21 ± 0.018'e karşılık 4.69 ±...

Unenriched skin microbiota transplantation for cats: new road story for treating feline atopic skin syndrome

“…All cats underwent dermatological examinations and associated tests, including DermLite DL4 dermatoscopy, skin scraping, acetate tape impression smear, skin cytology, Polycheck IgE tests (with the Turkish distributor being the RDA Group, Istanbul), and epidermal corneometric analyses using the Callegari Soft Plus Device (Italy). Diagnoses were based on the aforementioned tests ( Ural et al, 2018 ; Ural et al, 2019a , 2019b ; Ural et al, 2021 ). Throughout the trial, all cats were maintained on a low carbohydrate diet (17% from Virbac Digestive commercial food).…”

“…The latter algorithm involved novel terminology regarding FASS, establishing updated denotation of allergy, gastrointestinal and respiratory disorders among cats (Mueller et al, 2021). Under this classification FASS was dedicated to allergic dermatological issues in relationship with environmental allergy ( Santoro et al, 2021 ; Ural et al, 2019a , 2019b ; Ural et al, 2022a , 2022b ; Ural et al, 2023 ). For detailed diagnosis of FASS, classification ( Santoro et al, 2021 ) was performed in agreement with other data ( Diesel & DeBoer, 2011 ; Favrot et al, 2014 ; Ravens et al, 2014 ).…”