House dust mite extract induces growth factor expression in nasal mucosa by activating the PI3K/Akt/HIF-1α pathway

Chen, Xi; Li, Yingying; Zhang, Weiqiang; Zhang, Weiming; Zhou, Han

doi:10.1016/j.bbrc.2015.12.110

Cited by 12 publications

(14 citation statements)

References 25 publications

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“…Therefore, it would be much easier and more effective to extract PBMCs from blood samples, which are primarily used for routine blood monitoring of children with asthma. Furthermore, compared with the widely used lipopolysaccharide (LPS) stimulation, 15 HDM is a more common allergen for asthma attacks in humans and in animal models 16,17 . However, there are several limitations of this study.…”

Section: Discussionmentioning

confidence: 94%

“…Furthermore, compared with the widely used lipopolysaccharide (LPS) stimulation, 15 HDM is a more common allergen for asthma attacks in humans and in animal models. 16,17 However, there are several limitations of this study. First, the quantity of samples used for RNA-Seq was small, which may result in ignoring potential molecules due to the large data variations.…”

Section: Induction Of Il-1 Signaling Components In Asthmatic Micementioning

confidence: 93%

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IL‐1 signaling pathway molecules as key markers in childhood asthma

Wei

Huang

et al. 2020

Pediatric Allergy Immunology

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Objective The prevalence of childhood asthma has been increasing in recent years. This study aims to investigate the involvement of the key molecules of IL‐1 (interleukin‐1) signaling pathways in pediatric patients with asthma. Methods Differentially expressed genes (DEGs) associated with IL‐1 signaling pathways were identified with RNA‐seq from peripheral blood samples collected from asthmatic or healthy children and were further verified in clinical peripheral blood samples. Cellular models and asthmatic mice were subsequently developed to validate the identified asthmatic markers. Results Among the DEGs identified by RNA‐seq, eight signal transducers associated with the IL‐1 signaling network, namely IL‐1RN, IL‐1β, IL‐1RAP, IRAK3, IL‐1R1, MYD88, IRAK2, and PELI1, were found to be substantially upregulated in children with asthma. Interestingly, a significant serially increased expression of four genes (IL‐1RN, IL‐1RAP, IRAK3, and PELI1) was observed in healthy subjects, patients with chronic persistent asthma and patients with acute exacerbation asthma. In particular, these four genes were continuously overexpressed in recurrent patients. A significant induction of the above four genes was then observed in house dust mite (HDM)–stimulated peripheral blood mononuclear cells (PBMCs) and ovalbumin (OVA)–induced asthmatic mice. In addition, a time‐dependent induction of IL‐1RAP and PELI1 was also detected in HDM‐treated THP‐1 cells, an acute monocytic leukemia cell line. Conclusions These results demonstrate that IL‐1RN, IL‐1RAP, IRAK3, and PELI1, which are signal transducers of the IL‐1 signaling pathway, could serve as biomarkers for the pathogenesis of childhood asthma and for potential therapeutic targets of asthma.

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Section: Discussionmentioning

confidence: 94%

Section: Induction Of Il-1 Signaling Components In Asthmatic Micementioning

confidence: 93%

IL‐1 signaling pathway molecules as key markers in childhood asthma

Wei

Huang

et al. 2020

Pediatric Allergy Immunology

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“…More recently, Chen et al 42 . demonstrated that DM extract induced the expression of VEGF, transforming growth factor-β1 (TGF-β1), and fibroblast growth factor-2 (FGF-2) by activating the PI3K/Akt/HIF-1a pathway in human primary cultured nasal epithelia cells (NECs) and in the nasal mucosa of a murine model 42 . Furthermore, the demonstration that the expression of VEGFR1 and VEGF-B correlates with edema and clinical markers of nasal polyps (NP) disease is noteworthy, particularly as edema represents a key feature of NP disease, and therefore represents potential therapeutic targets 43 .…”

Section: Discussionmentioning

confidence: 98%

“…VEGF levels have been shown to be increased in the nasal mucosa of patients with AR 40 and contribute to nasal inflammation in response to allergen exposure 41 42 . demonstrated that DM extract induced the expression of VEGF, transforming growth factor-β1 (TGF-β1), and fibroblast growth factor-2 (FGF-2) by activating the PI3K/Akt/HIF-1a pathway in human primary cultured nasal epithelia cells (NECs) and in the nasal mucosa of a murine model 42 .…”

Section: Discussionmentioning

confidence: 99%

Identification of rare variants of allergic rhinitis based on whole genome sequencing and gene expression profiling: A preliminary investigation in four families

Zhang

Zhao

et al. 2019

World Allergy Organization Journal

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Background Despite the success of genome-wide association studies for allergic rhinitis (AR), no definitive causal variants have been identified, and a substantial portion of the heritability of the disease is yet to be discovered. Methods Four families, each with at least 1 parent and one child suffering from dust mite (DM) AR, were recruited, and whole-genome sequencing was performed on samples from 9 eligible individuals from these families. Conjoint analysis was performed for existing gene expression profiling data in the literature and the whole genome sequencing data obtained for these individuals; for presence of family-specific variants segregating with AR and the pathways involved. Similar analyses were also performed with data obtained for 96 sporadic house dust mite (HDM) AR patients and 96 healthy controls. Results Three rare variants in three genes (FLT1_c.603A > T; VEGFB_c.322A > C; and ITGA2_c.502+1G > A), which are involved in Focal Adhesion pathway, were identified in affected, but not unaffected, subjects in two families. VEGFB_c.322A > C and/or ITGA2_c.502+1G > A were further detected in all DM AR patients but not in any healthy individuals in 1 family; which was further investigated for members. The 3 identified variants were not found in any of the sporadic DM AR patients or healthy controls. Conclusion Despite the relatively small sample size, this study has identified several potentially functional rare variants in AR candidate genes, and it provides a platform for future work in larger numbers of families and sporadic individuals for a better understanding of the genetic basis of AR.

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“…[ 53 ] House dust mite extract induced the expression of growth factors in the nasal mucosa by activating the hif-1 PCR/PI3K/Akt pathway. [ 54 ] Studies also showed that miR-126 accelerated IgE-mediated MC degranulation associated with the PI3K/Akt signaling pathway by promoting Ca 2+ influx. [ 55 ] Overall, YPFP may regulate immunological functions through these pathways in AR treatment.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms and molecular targets of the Yu-Ping-Feng powder for allergic rhinitis, based on network pharmacology

Yang

Deng

et al. 2021

Medicine

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In traditional Chinese medicine (TCM), Yu-Ping-Feng powder (YPFP) has been used to treat allergic rhinitis (AR) for centuries. However, the mechanisms underlying its effects or its molecular targets in AR treatment are yet to be elucidated. Therefore, the active compounds of YPFP and their targets were collected and identified from the Traditional Chinese Medicine Systems Pharmacology database. Moreover, AR-associated targets were acquired from the GeneCards and Online Mendelian Inheritance in Man database. Proteins interactions network of YPFP presumed targets and AR-associated targets were examined and merged to reveal the candidate YPFP targets against AR. Cytoscape software and BisoGenet Database were employed to perform the Visualization and Integrated Discovery (Cluster Profiler R package, version: 3.8.1). Kyoto Encyclopedia of Genes and Genomes and genome pathway analyses. To identify the key target genes, a gene-pathway network has been constructed. We identified 44 effective active compounds and 622 YPFP targets. Also 1324 target genes related to AR were identified. Twenty pathways, including those of AGE-RAGE signaling, fluid shear stress, atherosclerosis, PI3K-Akt signaling, and tumor necrosis factor signaling was enriched significantly. MAPK1 was identified as the core gene, while others including RELA, AKT1, NFKBIA, IL6, and JUN, were also important in the gene-pathway network. Clearly, network pharmacology can be applied in revealing the molecular targets and mechanisms of action of complex herbal preparations. These findings suggested that YPFP could treat AR by regulating immunological functions, diminishing inflammation, and improving immunity through different pathways.

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House dust mite extract induces growth factor expression in nasal mucosa by activating the PI3K/Akt/HIF-1α pathway

Cited by 12 publications

References 25 publications

IL‐1 signaling pathway molecules as key markers in childhood asthma

IL‐1 signaling pathway molecules as key markers in childhood asthma

Identification of rare variants of allergic rhinitis based on whole genome sequencing and gene expression profiling: A preliminary investigation in four families

Mechanisms and molecular targets of the Yu-Ping-Feng powder for allergic rhinitis, based on network pharmacology

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