Hotspots of homologous recombination in mouse meiosis

Shiroishi, Toshihiko; Koide, Tsuyoshi; Yoshino, Masayasu; Sagai, Tomoko; Moriwaki, Kazuo

doi:10.1016/0065-227x(95)99387-5

Cited by 45 publications

(31 citation statements)

References 21 publications

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“…Besides these large-scale differences in CO number, the local distribution of CO can also depend on the DNA sequence. This is illustrated by the fact that several CO hotspots identified in the mouse MHC are specific for one or a few haplotypes (Shiroishi et al 1995). Similarly, at several human CO hotspots, the CO rate was found to vary widely from one individual to another (Neumann & Jeffreys 2006).…”

Section: Variations In Co Activitymentioning

confidence: 93%

Regulating double-stranded DNA break repair towards crossover or non-crossover during mammalian meiosis

2007

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During meiosis the programmed induction of DNA double-stranded breaks (DSB) leads to crossover (CO) and non-crossover products (NCO). One key role of CO is to connect homologs before metaphase I and thus to ensure the proper reductional segregation. This role implies an accurate regulation of CO frequency with the establishment of at least one CO per chromosome arm. Current major challenges are to understand how CO and NCO formation are regulated and what is the role of NCO. We present here the current knowledge about CO and NCO and their regulation in mammals. CO density varies widely along chromosomes and their distribution is not random as they are subject to positive interference. As documented in the mouse and human, a significant excess of DSB are generated relative to the number of CO. In fact, evidence has been obtained for the formation of NCO products, for regulation of the choice of DSB repair towards CO or NCO and for a CO specific pathway. We discuss the roles of Msh4, Msh5 and Sycp1 which affect DSB repair and probably not only the CO pathway. We suggest that, in mammals, the regulation of NCO differs from that described in Saccharomyces cerevisiae.

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Section: Variations In Co Activitymentioning

confidence: 93%

Regulating double-stranded DNA break repair towards crossover or non-crossover during mammalian meiosis

2007

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“…To confirm and measure the frequency of the meiotic crossovers within the Idd11 haplotype, 723 F 2 progeny were generated by intercrossing heterozygous (NOD 3 Idd11D)F 1 mice and were screened for recombination events between D4Wehi5 and D4Wehi6. The calculated crossover activity was ;50 cM Mb -1 , which is 100-fold greater than the mouse genome average (;0.5 cM Mb -1 ) (Shiroishi et al 1995), and confirms that this interval harbors a recombination hotspot (Fig. 2).…”

Section: Confirmation Of a Recombination Hotspotmentioning

confidence: 57%

“…Above each bar, the number of crossovers observed in that marker interval is shown. The dashed line indicates the average crossover activity for the mouse genome: ;0.5cM/Mb (Shiroishi et al 1995). Genetic markers are indicated by ticks above the plot.…”

Section: Discussionmentioning

confidence: 99%

A recombination hotspot leads to sequence variability within a novel gene (AK005651) and contributes to type 1 diabetes susceptibility

Tan

Mackin²,

Wang³

et al. 2010

Genome Res.

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More than 25 loci have been linked to type 1 diabetes (T1D) in the nonobese diabetic (NOD) mouse, but identification of the underlying genes remains challenging. We describe here the positional cloning of a T1D susceptibility locus, Idd11, located on mouse chromosome 4. Sequence analysis of a series of congenic NOD mouse strains over a critical 6.9-kb interval in these mice and in 25 inbred strains identified several haplotypes, including a unique NOD haplotype, associated with varying levels of T1D susceptibility. Haplotype diversity within this interval between congenic NOD mouse strains was due to a recombination hotspot that generated four crossover breakpoints, including one with a complex conversion tract.

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“…[1][2][3][4][5][6] Signals in site-specific pathways define the points at which the exchange will occur. General signals do not promote defined rearrangements but rather create hotspots by increasing the frequency of homologous recombination in their vicinity.…”

Section: Introductionmentioning

confidence: 99%

“…The recombination hotspots observed in higher eukaryotes typically lie near regions of highly repetitive sequences and are generally observed in the context of meiosis. [3][4][5] The existence of ubiquitous, general somatic recombination signals has been proposed, 5 but nothing comparable to chi has yet been functionally identified.…”

Section: Introductionmentioning

confidence: 99%

Capture of a recombination activating sequence from mammalian cells

Olson

Dornburg

1999

Gene Ther

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We have developed a genetic trap for identifying revealed a putative recombination signal (CCCACCC). sequences that promote homologous DNA recombination.When this heptamer or an abbreviated form (CCCACC) The trap employs a retroviral vector that normally disables were reinserted into the vector, they stimulated vector itself after one round of replication. Insertion of defined repair and other DNA rearrangements. Mutant forms of DNA sequences into the vector induced the repair of a 300 these oligomers (eg CCCAACC or CCWACWS) did not. base pair deletion, which restored its ability to replicate.Our data suggest that the recombination events occurred Tests of random sequence libraries made in the vector within 48 h after transfection.

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Hotspots of homologous recombination in mouse meiosis

Cited by 45 publications

References 21 publications

Regulating double-stranded DNA break repair towards crossover or non-crossover during mammalian meiosis

Regulating double-stranded DNA break repair towards crossover or non-crossover during mammalian meiosis

A recombination hotspot leads to sequence variability within a novel gene (AK005651) and contributes to type 1 diabetes susceptibility

Capture of a recombination activating sequence from mammalian cells

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