Host Tissue Interaction, Fate, and Risks of Degradable and Nondegradable Gel Fillers

Christensen, Lise

doi:10.1111/j.1524-4725.2009.01338.x

Cited by 135 publications

(102 citation statements)

References 34 publications

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“…Over the past two decades there have been an increasing number of injectable fillers based on chemically modified biomaterials to use for soft tissue wound repair. 1 The next generation of sECM-based materials, such as the Carbylan-GSX investigated in this study, is of potential interest because it allows for an injectable cellseeded construct that is capable of cross-linking in situ for local delivery in wound healing and other tissue engineering strategies. [31][32][33] In this study, we developed a novel coculture system to investigate the potential immunomodulatory and antiinflammatory properties of such cell-hydrogel constructs.…”

Section: Discussionmentioning

confidence: 99%

“…Most notable are commercially available HA-based materials for injectable soft tissue fillers, coating materials on implantable devices, or dermal replacement grafts. 1 Hyaluronan is a vital element of the extracellular matrix (ECM), providing tissues with an optimal environment for cell attachment, migration, and growth. 2 In addition, HA is an important player in the inflammatory cascade, not only recruiting immune cells to the site of wounded tissue but contributing to granulation tissue formation as well.…”

mentioning

confidence: 99%

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The Effect of Mesenchymal Stromal Cell–Hyaluronic Acid Hydrogel Constructs on Immunophenotype of Macrophages

Hanson

King

Kim

et al. 2011

Tissue Engineering Part A

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During the past several years, multipotent mesenchymal stromal cells (MSCs) have rapidly moved from in vitro and animal studies into clinical trials as a therapeutic modality potentially applicable to a wide range of disorders. It has been proposed that ex vivo culture-expanded MSCs exert their tissue regeneration potential through their immunomodulatory and anti-inflammatory properties, and paracrine effects more than their ability to differentiate into multiple tissue lineages. Since extracellular matrix (ECM) deposition and tissue support is also one of many physiological roles of MSCs, there is increasing interest in their potential use for tissue engineering, particularly in combination with ECM-based scaffolds such as hyaluronic acid (HA). We investigated the effect of MSCs on immunophenotype of macrophages in the presence of an HA-hydrogel scaffold using a unique 3D coculture system. MSCs were encapsulated in the hydrogel and peripheral blood CD14 + monocyte-derived macrophages plated in direct contact with the MSC-gel construct. To determine the immunophenotype of macrophages, we looked at the expression of cell surface markers CD14, CD16, CD206, and human leukocyte antigen (HLA)-DR by flow cytometry. MSCs and macrophages cultured on the HAhydrogel remained viable and were able to be recovered from the construct. There was a significant difference in the immunophenotype observed between monocyte-derived macrophages cultured on the HA scaffold compared to tissue culture polystyrene. Macrophages cultured on gels with MSCs expressed lower CD16 and HLA-DR with higher expression of CD206, indicating the least inflammatory profile overall, compatible with the immunophenotype of alternatively activated macrophages. Development of macrophages, with this immunophenotype, upon interaction with the MSC-hydrogel constructs may play a potentially significant role in tissue repair when using a cellular-biomaterial therapeutic approach.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

The Effect of Mesenchymal Stromal Cell–Hyaluronic Acid Hydrogel Constructs on Immunophenotype of Macrophages

Hanson

King

Kim

et al. 2011

Tissue Engineering Part A

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“…In response, Vordermark et al proposed that a material with a faster resolution would be suitable for application to HDRIBT [Vordermark, 2008]. These long-lasting hyaluronate implants may cause immune [Hamilton, 2007] or inflammatory reactions [Arron, 2007], or infections [Christensen, 2009] [Arron, 2007;Edwards, 2007;Ghislanzoni, 2006;Wiest, 2009;Wolfram, 2006], and have been surgically removed from some patients [Arron, 2007;Edwards, 2007;Ghislanzoni, 2006;Wiest, 2009;Wolfram, 2006]. 2.…”

Section: Interval Repairmentioning

confidence: 99%

“…1) [Kishi, 2004、2006, 2007a, 2007b, 2009, Prada, 2007, 2009Vordermark, 2008]. In the first stage of our strategy, we aimed to treat recurrent disease requiring reirradiation with single-session high-dose-rate brachytherapy (HDRBT), reporting an effective decrease in estimated cumulative normal tissue complication probabilities (NTCP) to safer levels.…”

mentioning

confidence: 99%

Safe and Curative Brachytherapy Reirradiation with Organ-Sparing Hyaluronate Gel Injection

Kishi¹,

Noda²,

Sato³

2012

Brachytherapy

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“…Further, even if the risk of bacterial biofilm development on the injected filling agent and virulence are rare, these potential complications must be taken into consideration. 6 This report describes a rare case of bacterial biofilm formation on hyaluronic acid filler deeply injected into the cheeks, probably spread from improper endodontic treatment of tooth 16 (first molar).…”

Section: Introductionmentioning

confidence: 99%

Probable biofilm formation in the cheek as a complication of soft tissue filler resulting from improper endodontic treatment of tooth 16

Marusza

Młynarczyk²,

Olszański³

et al. 2012

IJN

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Injectable filling agents offer the promise of a better appearance without surgery and, among them, hyaluronic acid is the most commonly used. Although complications are rare, it is necessary to know the possible side effects and complications in order to be prepared for their management. That is why many researchers have been focusing on the interactions between hyaluronic acid and pathogens, inflammatory mediators, the immune system, and markers of oxidative stress to achieve efficient drug delivery, given that hyaluronic acid has widening applications in the field of nanomedicine. Here we report the case of a 37-year-old female patient who returned to our clinic with an abscess in her left cheek 3 months after a deep injection of 1 mL of stabilized hyaluronic acid in both cheeks. Steroid and antibiotic therapy was initiated without success, and abscess drainage was performed. Extraction of tooth 16 was performed 11 days after insertion of drains into the abscess. Laboratory blood tests showed acute inflammation of presumed bacterial etiology. Microbiological examination of pus was negative. Bacterial cultures were found in the extracted tooth. After antibiotic therapy, a complete reversal of the pathological process was observed. The present report highlights the need to assess periodontal problems prior to any aesthetic facial treatment. Analyses of further case reports and clinical studies are necessary to understand the potential role of hyaluronic acid in the formation of biofilm, and how to avoid this complication, thereby increasing the safety of hyaluronic acid-based procedures.

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Host Tissue Interaction, Fate, and Risks of Degradable and Nondegradable Gel Fillers

Cited by 135 publications

References 34 publications

The Effect of Mesenchymal Stromal Cell–Hyaluronic Acid Hydrogel Constructs on Immunophenotype of Macrophages

The Effect of Mesenchymal Stromal Cell–Hyaluronic Acid Hydrogel Constructs on Immunophenotype of Macrophages

Safe and Curative Brachytherapy Reirradiation with Organ-Sparing Hyaluronate Gel Injection

Probable biofilm formation in the cheek as a complication of soft tissue filler resulting from improper endodontic treatment of tooth 16

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