Host-switching by a vertically transmitted rhabdovirus in
            <i>Drosophila</i>

Longdon, Ben; Wilfert, Lena; Osei-Poku, Jewelna; Cagney, Heather; Obbard, Darren J.; Jiggins, Francis M.

doi:10.1098/rsbl.2011.0160

Cited by 26 publications

(36 citation statements)

References 29 publications

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“…The novel viruses were highly divergent from known viruses. Sigma viruses known from other species of Drosophila typically have genomes of ∼12.5 kb (Longdon, Obbard, and Jiggins 2010; Longdon et al. 2011b), and six of our sequences were approximately this size, suggesting they are near- complete genomes.…”

Section: Resultsmentioning

confidence: 99%

The evolution, diversity, and host associations of rhabdoviruses

et al. 2015

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Metagenomic studies are leading to the discovery of a hidden diversity of RNA viruses. These new viruses are poorly characterized and new approaches are needed predict the host species these viruses pose a risk to. The rhabdoviruses are a diverse family of RNA viruses that includes important pathogens of humans, animals, and plants. We have discovered thirty-two new rhabdoviruses through a combination of our own RNA sequencing of insects and searching public sequence databases. Combining these with previously known sequences we reconstructed the phylogeny of 195 rhabdovirus sequences, and produced the most in depth analysis of the family to date. In most cases we know nothing about the biology of the viruses beyond the host they were identified from, but our dataset provides a powerful phylogenetic approach to predict which are vector-borne viruses and which are specific to vertebrates or arthropods. By reconstructing ancestral and present host states we found that switches between major groups of hosts have occurred rarely during rhabdovirus evolution. This allowed us to propose seventy-six new likely vector-borne vertebrate viruses among viruses identified from vertebrates or biting insects. Based on currently available data, our analysis suggests it is likely there was a single origin of the known plant viruses and arthropod-borne vertebrate viruses, while vertebrate- and arthropod-specific viruses arose at least twice. There are also few transitions between aquatic and terrestrial ecosystems. Viruses also cluster together at a finer scale, with closely related viruses tending to be found in closely related hosts. Our data therefore suggest that throughout their evolution, rhabdoviruses have occasionally jumped between distantly related host species before spreading through related hosts in the same environment. This approach offers a way to predict the most probable biology and key traits of newly discovered viruses.

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Section: Resultsmentioning

confidence: 99%

The evolution, diversity, and host associations of rhabdoviruses

et al. 2015

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“…Possible host switching events have previously been identified in at least two genera of rhabdoviruses. 41,42 However, sampling of more viruses from each of these subgroups is required before firm conclusions on natural host range can be drawn. Furthermore, although some ledanteviruses have been isolated from arthropods and the clade sits within the dimarhabdovirus supergroup, it is not clear at this time if vector-borne transmission is a common characteristic of all viruses in the proposed new genus.…”

Section: Discussionmentioning

confidence: 99%

Ledantevirus: A Proposed New Genus in the Rhabdoviridae has a Strong Ecological Association with Bats

Blasdell¹,

Guzmán²,

Widen³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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The Le Dantec serogroup of rhabdoviruses comprises Le Dantec virus from a human with encephalitis and Keuriliba virus from rodents, each isolated in Senegal. The Kern Canyon serogroup comprises a loosely connected set of rhabdoviruses many of which have been isolated from bats, including Kern Canyon virus from California, Nkolbisson virus from Cameroon, Central African Republic, and Cote d'Ivoire, Kolente virus from Guinea, Mount Elgon bat and Fikirini viruses from Kenya, and Oita virus from Japan. Fukuoka virus isolated from mosquitoes, midges, and cattle in Japan, Barur virus from a rodent in India and Nishimuro virus from pigs in Japan have also been linked genetically or serologically to this group. Here, we analyze the genome sequences and phylogenetic relationships of this set of viruses. We show that they form three subgroups within a monophyletic group, which we propose should constitute the new genus Ledantevirus.

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“…Over 200 rhabdoviruses have been identified (31), and a majority of the plant rhabdoviruses and some vertebrate rhabdoviruses use an insect vector for transmission. However, only a few rhabdoviruses have been directly isolated from insects or insect cell lines; these belong to the order Diptera (such as sigma viruses in Drosophila and Muscina flies [26,32,33] and the Culex tritaeniorhynchus rhabdovirus [CTRV] in Culex mosquitoes [34]) and to the order Hemiptera (such as Taastrup virus in the leafhopper [25]). We report here the discovery of a novel rhabdovirus in the Spodoptera frugiperda Sf9 cell line, which is the first rhabdovirus found in the order Lepidoptera.…”

Section: Discussionmentioning

confidence: 99%

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

Galvin

Glasner

et al. 2014

J Virol

127

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The Sf9 cell line, derived from Spodoptera frugiperda, is used as a cell substrate for biological products, and no viruses have been reported in this cell line after extensive testing. We used degenerate PCR assays and massively parallel sequencing ( IMPORTANCEThe Spodoptera frugiperda Sf9 cell line is used as a cell substrate for the development and manufacture of biological products. Extensive testing has not previously identified any viruses in this cell line. This paper reports on the identification and characterization of a novel rhabdovirus in Sf9 cells. This was accomplished through the use of next-generation sequencing platforms, de novo assembly tools, and extensive bioinformatics analysis. Rhabdovirus identification was further confirmed by transmission electron microscopy. Infectivity studies showed the lack of replication of Sf-rhabdovirus in human cell lines. The overall study highlights the use of a combinatorial testing approach including conventional methods and new technologies for evaluation of cell lines for unexpected viruses and use of comprehensive bioinformatics strategies for obtaining confident next-generation sequencing results.

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Host-switching by a vertically transmitted rhabdovirus in Drosophila

Cited by 26 publications

References 29 publications

The evolution, diversity, and host associations of rhabdoviruses

The evolution, diversity, and host associations of rhabdoviruses

Ledantevirus: A Proposed New Genus in the Rhabdoviridae has a Strong Ecological Association with Bats

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

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