Host-suitability of black medick (Medicago lupulina L.) and additional molecular markers for identification of the pea cyst nematode Heterodera goettingiana

Vovlas, Alessio; Santoro, Simona; Radicci, V.; Leonetti, Paola; Castillo, Pablo; Palomares-Rius, Juan E.

doi:10.1007/s10658-017-1153-5

Cited by 5 publications

(6 citation statements)

References 11 publications

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“…Heterodera carotae is morphologically and genetically very close to the cabbage cyst nematode H. cruciferae , and the correct diagnosis is difficult and time consuming. rRNA and mitochondrial molecular markers such as ITS, cytochrome c oxidase 1 subunit ( coxI ) region (Vovlas et al , ; Escobar‐Avila et al , ) and coxI ‐derived primer sets (Madani et al , ) can help in the identification of members of the Goettingiana group, but none are able to provide an unequivocal distinction between H. carotae and H. cruciferae . As a result, only a host range test using carrot can distinguish these two species accurately (Escobar‐Avila et al , ).…”

Section: Discussionmentioning

confidence: 99%

Evidence of strong gene flow among French populations of the carrot cyst nematode Heterodera carotae

et al. 2019

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The recent ban of the most efficient chemical nematicides has left growers without methods for controlling the carrot cyst nematode Heterodera carotae. This phytoparasitic nematode species has a very narrow host range and causes severe crop losses in the main carrot‐growing regions worldwide. The development of alternative means of management of H. carotae is thus essential, and knowledge is required about the adaptive abilities of H. carotae, which mainly depend on gene flow among populations. The goal of this study was to describe the genetic structure of H. carotae populations at the spatial scale of the main infested French carrot‐producing region, i.e. Lower Normandy, and to disentangle the causes of the heterozygote deficit in this polyvoltine species. Microsatellite genotyping of populations collected at both the plant and field scales showed that: (i) the heterozygote deficit is mainly due to substructure; and (ii) there is strong gene flow among populations, leading to low FST and to no clear genetic structure at the spatial scale explored here. Soil transport through both agricultural machinery and the transport of leek seedlings is probably responsible for the very strong H. carotae migration among fields and production areas. Measures should be considered to limit the passive spread of H. carotae.

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Section: Discussionmentioning

confidence: 99%

Evidence of strong gene flow among French populations of the carrot cyst nematode Heterodera carotae

et al. 2019

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“…The newly obtained sequences for each gene (ITS-rDNA, D2-D3 region of 28S-rDNA, and COI gene) were compared with known sequences of Heterodera using BLASTn homology search program. Outgroup taxa for phylogenetic analyses were selected based on the previously published studies ( Subbotin et al, 2001 ; Maafi et al, 2003 ; Mundo-Ocampo et al, 2008 ; Kang et al, 2016 ; Madani et al, 2018; Vovlas et al, 2017 ). The selected sequences were aligned by MAFFT ( Kazutaka and Standley, 2013 ) with default parameters and edited using Gblock ( Castresana, 2000 ).…”

Section: Methodsmentioning

confidence: 99%

“…Due to overlapping morphological characters and phenotypic plasticity, it is difficult to distinguish closely related Heterodera species; therefore, sequence-based diagnosis is gaining more reliability for precise and accurate identification of cyst-forming nematodes ( Peng et al, 2003 ). The internal transcribed spacer region of the ribosomal DNA (ITS-rDNA), the D2 and D3 expansion fragments of the 28S ribosomal DNA genes (D2-D3 of 28S-rDNA), and mitochondrial DNA ( COI gene) units are good candidate genes for molecular taxonomic and phylogenetic studies ( Subbotin et al, 2001; Subbotin et al, 2006 ; Madani et al, 2004 ; Vovlas et al, 2017 ). Based on morphomolecular characterizations, Handoo and Subbotin (2018) divided Heterodera into nine distinct groups such as Afenestrata , Avenae , Bifenestra , Cardiolata , Cyperi , Goettingiana , Humuli , Sacchari , and Schachtii group.…”

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confidence: 99%

Description of Heterodera microulae sp. n. (Nematoda: Heteroderinae) from China – a new cyst nematode in the Goettingiana group

et al. 2020

Journal of Nematology

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A new cyst-forming nematode, Heterodera microulae sp. n., was isolated from the roots and rhizosphere soil of Microula sikkimensis in China. Morphologically, the new species is characterized by lemon-shaped body with an extruded neck and obtuse vulval cone. The vulval cone of the new species appeared to be ambifenestrate without bullae and a weak underbridge. The second-stage juveniles have a longer body length with four lateral lines, strong stylets with rounded and flat stylet knobs, tail with a comparatively longer hyaline area, and a sharp terminus. The phylogenetic analyses based on ITS-rDNA, D2-D3 of 28S rDNA, and COI sequences revealed that the new species formed a separate clade from other Heterodera species in Goettingiana group, which further support the unique status of H. microulae sp. n. Therefore, it is described herein as a new species of genus Heterodera; additionally, the present study provided the first record of Goettingiana group in Gansu Province, China.

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“…Cyst nematode populations are widespread in Europe, Russia and the Mediterranean belt [ 63 ]. Besides peas, many legume species are suitable hosts of this nematode, including faba bean, vetch and black medick [ 167 ]. Infection is promoted by non-aerated soil, a suitable moist climate and host plant vulnerability.…”

Section: Pea Rhizospheric Diseasesmentioning

confidence: 99%

“…It was reported [ 63 ] that the utilization of longer rotation systems in the absence of host plants for up to 6 years can drastically reduce cyst nematode populations. However, for the success of rotations, a better understanding of the host range of nematodes is required to prevent incubation in the absence of the main host plant [ 167 ]. The incorporation of 10% aldicarb nematicides into infected soil before planting has been reported to control H. goettingiana in pea [ 170 ].…”

Section: Pea Rhizospheric Diseasesmentioning

confidence: 99%

Pea Breeding for Resistance to Rhizospheric Pathogens

Wohor

Rispail²,

Ojiewo³

et al. 2022

Plants

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Pea (Pisum sativum L.) is a grain legume widely cultivated in temperate climates. It is important in the race for food security owing to its multipurpose low-input requirement and environmental promoting traits. Pea is key in nitrogen fixation, biodiversity preservation, and nutritional functions as food and feed. Unfortunately, like most crops, pea production is constrained by several pests and diseases, of which rhizosphere disease dwellers are the most critical due to their long-term persistence in the soil and difficulty to manage. Understanding the rhizosphere environment can improve host plant root microbial association to increase yield stability and facilitate improved crop performance through breeding. Thus, the use of various germplasm and genomic resources combined with scientific collaborative efforts has contributed to improving pea resistance/cultivation against rhizospheric diseases. This improvement has been achieved through robust phenotyping, genotyping, agronomic practices, and resistance breeding. Nonetheless, resistance to rhizospheric diseases is still limited, while biological and chemical-based control strategies are unrealistic and unfavourable to the environment, respectively. Hence, there is a need to consistently scout for host plant resistance to resolve these bottlenecks. Herein, in view of these challenges, we reflect on pea breeding for resistance to diseases caused by rhizospheric pathogens, including fusarium wilt, root rots, nematode complex, and parasitic broomrape. Here, we will attempt to appraise and harmonise historical and contemporary knowledge that contributes to pea resistance breeding for soilborne disease management and discuss the way forward.

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Host-suitability of black medick (Medicago lupulina L.) and additional molecular markers for identification of the pea cyst nematode Heterodera goettingiana

Cited by 5 publications

References 11 publications

Evidence of strong gene flow among French populations of the carrot cyst nematode Heterodera carotae

Evidence of strong gene flow among French populations of the carrot cyst nematode Heterodera carotae

Description of Heterodera microulae sp. n. (Nematoda: Heteroderinae) from China – a new cyst nematode in the Goettingiana group

Pea Breeding for Resistance to Rhizospheric Pathogens

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