Host specificities of RNA phages

Dennison, Sheena; Hedges, R. W.

doi:10.1017/s0022172400022099

Cited by 17 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Exceptions occur with plasmids of IncF subgroups I-IV which constitute the F-complex. The pili coded for by these groups of plasmids are serologically related (Lawn & Meynell, 1970) and some RNA phages like MS2 or Qp plate on strains carrying plasmids belonging to any of these groups (Dennison & Hedges, 1972). Phage pilHor, by plating on bacterial strains carrying either HI or HI1 plasmids (Table l) …”

Section: J N C O E T Z E E a N D O T H E R Smentioning

confidence: 99%

Phage pilH : a Phage Which Adsorbs to IncHI and IncHII Plasmid-coded Pili

et al. 1985

Self Cite

View full text Add to dashboard Cite

Phage pilHa was specific for bacterial strains, of various genera, harbouring plasmids of the HI and HII incompatibility groups. Plaque formation was temperature sensitive in that plaques formed at 26 °C but not at 37 °C. Plaques were fairly clear, irregular in outline and varied from pin point to about 2 mm in diameter on all hosts where plaques were detected. The phage had an isometric hexagonal outline with a diameter of 25 nm. It contained RNA but differed from all but one other plasmid-dependent RNA phage by being sensitive to chloroform. It adsorbed along the length of the shafts of IncHI and HII plasmid-coded pili.

show abstract

Section: J N C O E T Z E E a N D O T H E R Smentioning

confidence: 99%

Phage pilH : a Phage Which Adsorbs to IncHI and IncHII Plasmid-coded Pili

et al. 1985

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our finding that all I-complex plasmids tested are related in some degree to the 0 group suggests that group 0 and I plasmids have a common phylogenetic origin. Certainly, the failure of 0 plasmids to support the growth of phage If1 need not rule out the presence of I pili, since there is ample evidence with the F-like plasmids that closely related pili may differ significantly in serological properties (Lawn & Meynell, 1970) or by differential adsorption of isometric RNA phages and filamentous DNA phages (Meynell, Meynell & Datta, 1968;Dennison & Hedges, 1972). Hence, the pili specified by 0 group plasmids may be I-like in their transfer functions despite their inability to adsorb the one available class of I pilus-specific phage.…”

Section: S Falkow P G U E R R Y R W H E D G E S a N D N Dattamentioning

confidence: 99%

Polynucleotide Sequence Relationships among Plasmids of the I Compatibility Complex

Falkow¹,

Guerry²,

Hedges³

et al. 1974

Journal of General Microbiology

Self Cite

View full text Add to dashboard Cite

SUMMARYPlasmids determining I pili show a variety of intricate compatibility relationships. None the less, all I plasmids tested showed a 'core' of common DNA sequences of approximately 30 x 106 daltons. Plasmids of the 0 compatibility group do not appear to specify for the synthesis of I-like pili and yet they held about 20 yo of their sequences in common with all tested I plasmids, suggesting a common phylogenetic origin. Members of the I complex (including group 0) do not hold a significant proportion of their sequences in common with representative plasmids of other compatibility groups. We propose that the regions of similar polynucleotide sequences of the different I complex plasmids are due to phylogenetic homology between genes governing pili biosynthesis and/or transfer functions. The regions determining compatibility of the I complex plasmids in some cases may be either unrelated to or have diverged from a common ancestor.

show abstract

“…This conclusion is plausible since no plasmid determining constitutive I pilus production has been observed in nature and the maladaptive properties of such synthesis have been demonstrated (Dowman & Meynell, 1970;Romero et al, 1971 ;Salisbury et al, 1972). Hence, I pili are probably only rarely available and the range of phages adapted to adsorption to such receptors is likely to be limited, and far narrower than, for example, the F complex (Miyake et al, 1969;Dennison & Hedges, 1972). …”

Section: Discussionmentioning

confidence: 99%

Phages l and I2-2: IncI Plasmid-dependent Bacteriophages

1982

Self Cite

View full text Add to dashboard Cite

~ ~ ~~~~Phage Ia was isolated from sewage from Windhoek, South West Africa. It formed relatively clear plaques about 2 mm in diameter, on sensitive strains of Escherichia coli K 12 and Salmonellu typhimurium LT2. The phage had an hexagonal outline with a diameter of about 24 nm, contained RNA and was resistant to chloroform. Phage Ia formed plaques or propagated only on organisms carrying I, plasmids or the Iy plasmid R621a. The efficiency of plating was higher on E. coli than on S . typhimurium hosts. The phage adsorbed along the length of shafts of I, pili. Phage 12-2 was isolated from Pretoria sewage. It was a filamentous virus and individual virions varied considerably in length. Phage 12-2 formed turbid plaques which varied from pin point to about 1 mm in diameter on all hosts. It was resistant to RNAase and sensitive to chloroform. Phage 12-2 had a spectrum of activity limited to strains harbouring I2 plasmids but the adsorption site could not be demonstrated. The phage was not related serologically to phages If1 or PR64FS.

show abstract

Host specificities of RNA phages

Abstract: SUMMARYHost ranges of members of four groups of male-specific RNA coliphages were determined by plating on hosts carrying various derepressed plasmids. An RNA phage originally isolated onPseudomonas aeruginosafailed to form plaques on any of the strains ofEscherichia coli.

Cited by 17 publications

References 26 publications

Phage pilH : a Phage Which Adsorbs to IncHI and IncHII Plasmid-coded Pili

Phage pilH : a Phage Which Adsorbs to IncHI and IncHII Plasmid-coded Pili

Polynucleotide Sequence Relationships among Plasmids of the I Compatibility Complex

Phages l and I2-2: IncI Plasmid-dependent Bacteriophages

Contact Info

Product

Resources

About