Host-Specific Patterns of Genetic Diversity among IncI1-Iγ and IncK Plasmids Encoding CMY-2 β-Lactamase in Escherichia coli Isolates from Humans, Poultry Meat, Poultry, and Dogs in Denmark

Abstract

CMY-2 is the most common plasmid-mediated AmpC ␤-lactamase in Escherichia coli isolates of human and animal origin. The aim of this study was to elucidate the epidemiology of CMY-2-producing E. coli in Denmark. Strain and plasmid relatedness was studied in 93 CMY-2-producing clinical and commensal E. coli isolates collected from 2006 to 2012 from humans, retail poultry meat, broilers, and dogs. Multilocus sequence typing (MLST), antimicrobial susceptibility testing, and conjugation were performed in conjunction with plasmid replicon typing, plasmid multilocus sequence typing (pMLST), restriction fragment length polymorphism (RFLP), and sequencing of selected bla CMY-2 -harboring plasmids. MLST revealed high strain diversity, with few E. coli lineages occurring in multiple host species and sample types. bla CMY-2 was detected on plasmids in 83 (89%) isolates. Most (75%) of the plasmids were conjugative and did not (96%) cotransfer resistance to antimicrobials other than cephalosporins. The main replicon types identified were IncI1-I␥ (55%) and IncK (39%). Isolates from different host species mainly carried distinct plasmid subtypes. Seven of the 18 human isolates harbored IncI1-I␥/sequence type 2 (ST2), IncI1-I␥/ST12, or IncK plasmids highly similar to those found among animal isolates, even though highly related human and animal plasmids differed by nonsynonymous single nucleotide polymorphisms (SNPs) or insertion sequence elements. This study clearly demonstrates that the epidemiology of CMY-2 can be understood only by thorough plasmid characterization. To date, the spread of this ␤-lactam resistance determinant in Denmark is mainly associated with IncK and IncI1-I␥ plasmids that are generally distributed according to host-specific patterns. These baseline data will be useful to assess the consequences of the increasing human exposure to CMY-2-producing E. coli via animal sources. IMPORTANCECMY-2 is the most common plasmid-mediated AmpC ␤-lactamase in Escherichia coli. This ␤-lactamase is poorly inhibited by clavulanic acid and confers resistance to cephamycins, third-generation cephalosporins, and aztreonam. Furthermore, resistance to carbapenems has been reported in E. coli as a result of production of plasmid-encoded CMY-2 ␤-lactamase in combination with decreased outer membrane permeability. The gene encoding CMY-2 generally resides on transferable plasmids belonging to different incompatibility groups. The prevalence of CMY-2-mediated cephalosporin resistance in E. coli varies significantly depending on the geographical region and host. This study demonstrates that the epidemiology of CMY-2 can be understood only by thorough plasmid characterization. To date, the spread of this ␤-lactam resistance determinant in Denmark is mainly associated with IncK and IncI1-I␥ plasmids, which are generally distributed according to host-specific patterns. These data will be useful to assess the consequences of the increasing human exposure to CMY-2-producing E. coli via animal sources. C MY-2 is the most common p...

Antimicrobial Resistance inEscherichia coli

“…In animals, the majority of identified AmpC enzymes have been of the CMY type (Table 2) (25, 56). A recent study performed in Denmark identified CMY-2-producing E. coli isolates from poultry meat, poultry, and dogs (57). The study showed that the dissemination of bla CMY-2 was mainly due to the spread of IncI1-γ and IncK plasmids.…”

野生动物(野鸟)对全球耐药基因传播的影响

“…CMY-2 is the most prevalent AmpC β-lactamase and has been globally disseminated among Enterobacteriaceae in humans, companion animals, food-producing animals, and retail meat (Bogaerts et al, 2015; Carmo et al, 2014; Hansen et al, 2016; Jacoby, 2009; Ma et al, 2012; Smet et al, 2010; Vogt et al, 2014; Wu et al, 2015). As shown in Table 2, AmpC β-lactamases have been reported in E. coli , K. pneumoniae , and Enterobacter cloacae isolates of wildlife origin in Europe, North America, and Asia, and particularly in central Europe, similar to ESBL-producing Enterobacteriaceae (Table 2).…”

Plasmids Carrying blaCMY -2/4 in Escherichia coli from Poultry, Poultry Meat, and Humans Belong to a Novel IncK Subgroup Designated IncK2

“…Other studies investigating pAmpC-producing E. coli have detected the nearly identical kind of plasmid in healthy humans (Baudry et al, 2009; Porres-Osante et al, 2015; Hansen et al, 2016), poultry (Dierikx et al, 2010; Hansen et al, 2016; Mo et al, 2016), poultry meat (Egervärn et al, 2014), and dogs (Hansen et al, 2016). This prompted us to perform an in-depth analysis of bla CMY -2/4 -carrying IncB/O/K plasmids from E. coli isolated in three settings in Switzerland to determine their genetic relatedness, revealing a novel IncK-group variant, the IncK2.…”