Host Restriction of Friend Leukemia Virus. Role of the Viral Outer Coat

Krontiris, Theodore G.; Soeiro, Ruy; Fields, Bernard N.

doi:10.1073/pnas.70.9.2549

Cited by 81 publications

(40 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VSV pseudotype particles have been employed to study the biological properties of the envelope glycoproteins of avian leukosis and reticuloendotheliosis viruses (21)(22)(23)(24), murine leukemia viruses (21,25,26), murine mammary tumor virus (27,28), bovine leukosis virus (BLV) (29), and primate retroviruses (30,31). Here we report the production of VSV(HTLV) pseudotype particles and their neutralization by human sera.…”

mentioning

confidence: 99%

Pseudotypes of human T-cell leukemia virus types 1 and 2: neutralization by patients' sera.

Clapham¹,

Nagy²,

Weiss³

1984

Proc. Natl. Acad. Sci. U.S.A.

123

View full text Add to dashboard Cite

Pseudotypes of vesicular stomatitis virus (VSV) bearing envelope antigens of human T-cell leukemia virus (HTLV) types 1 and 2 were prepared by propagating VSV in cells lines productively infected with HTLV. Plaque assays of VSV(HTLV) pseudotypes were employed to determine the presence of (i) HTLV receptors on cells and (it) neutralizing antibodies in the serum of patients with adult T-cell leukemialymphoma (ATLL). Cell surface receptors for HTLV-1 and HTLV-2 were found on nonlymphoid cells of human and mammalian origin. Neutralizing antibodies specific to VSV(HTLV-1) were found in sera of ATLL patients in titers varying from 1:50 to 1:30,000 and did not correlate closely with antibody titers for internal viral antigens. Sera from ATLL patients in the United Kingdom (Caribbean immigrants), United States, and Japan completely neutralized VSV(HTLV-1), indicating that the HTLV isolates from these distinct geographic regions represent a single envelope serotype. Neutralization of VSV(HTLV-1) was more specific and more sensitive than assays of syncytium inhibition. No crossneutralization was observed between bovine leukosis virus and HTLV, and only limited cross-reaction was found for envelope antigens of HTLV-1 and HTLV-2. These studies show that VSV(HTLV) pseudotypes can be readily used to screen for neutralizing antibodies in patients' sera and to distinguish HTLV envelope serotypes.Human T-cell leukemia virus type 1 (HTLV-1) is a C-type retrovirus associated with certain malignancies of mature T cells known as adult T-cell leukemia-lymphoma (ATLL) (1, 2). The first isolates of HTLV were made from tumor cells of American patients (3,4). A virus formerly known as adult Tcell leukemia virus (ATLV) (5, 6), endemic in the regions of southwestern Japan where ATLL is prevalent (1,7,8) (19,20). We have adopted the VSV system for HTLV studies.When cells chronically infected with retroviruses are superinfected with VSV, a proportion of the progeny VSV particles bear the envelope glycoproteins of the retrovirus (20)(21)(22). Core antigens of the retrovirus are not assembled into VS virions. The VSV pseudotypes resist neutralization by anti-VSV antibodies but are sensitive to neutralizing antibodies specific to the retrovirus donating the envelope antigens. The host range for viral penetration of pseudotypes is restricted to cells bearing receptors for the retrovirus. Following penetration and uncoating, however, the VSV genome contained in the pseudotype particle replicates to produce nonpseudotype progeny. Thus, a cytopathic plaque assay of VSV pseudotypes can be used to determine receptor expression, receptor interference, and neutralizing antibodies specific to the retrovirus encoding the envelope glycoproteins.

show abstract

mentioning

confidence: 99%

Pseudotypes of human T-cell leukemia virus types 1 and 2: neutralization by patients' sera.

Clapham¹,

Nagy²,

Weiss³

1984

Proc. Natl. Acad. Sci. U.S.A.

123

View full text Add to dashboard Cite

show abstract

“…Studies on the mechanism of the Fv-i restriction have shown that the inhibition is intracellular (8)(9)(10)(11). Analysis of the fate of virus in heterokaryons of permissive and nonpermissive cells indicated that the restriction functions at some early postpenetration step in infection (I1).…”

mentioning

confidence: 99%

Reciprocal Inhibition of Mouse Leukemia Virus Infection by Fv-1 Allele Cell Extracts

Tennant

Schluter

Yang

et al. 1974

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…A parallel for this may be found in cells of a mouse strain, non-permissive for growth of N-tropic Friend leukaemia virus; while these cells are transformed by defective sarcoma virus (S + L-cells) they are rendered permissive since apparently the host-cell restriction of FLV is lost in transformed cells (Krontiris et al 1973).…”

Section: Discussionmentioning

confidence: 99%

A Transmissible Antigen Detected in Two Cell Lines Derived from Human Tumours

Závada

Závadova

Widmaier³

et al. 1974

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYVesicular stomatitis virus (VSV) can form 'pseudotype' particles with neutralization antigen(s) derived from mouse or chicken leukaemia viruses. These pseudotype particles contain the genome of VSV, but show neutralization, host-range and interference specificity of the corresponding oncornavirus. Pseudotypes were also detected following the growth of VSV in two established cell lines derived from human tumours: in MaTu derived from mammary carcinoma and in Tu-I35 derived from a sarcoma. No virus-like particles were detected in these cells by labelling with pH]-uridine followed by sucrose density gradient sedimentation. Media from these cultures did not contain DNA polymerase. Nevertheless, the capacity to produce VSV pseudotype was transmitted to some pseudotype-negative cells following their co-cultivation with X-irradiated, pseudotype-positive cells. Cell-free filtrates did not transmit the property. An antigen detected by immunofluorescence was also consistently transmitted. The VSV pseudotype produced in cells infected by co-cultivation possessed the same neutralization specificity as VSV pseudotype produced in the original tumour cells.

show abstract

Host Restriction of Friend Leukemia Virus. Role of the Viral Outer Coat

Abstract: Host restriction of oncogenesis of RNA tumor viruses in vivo is associated with several gene loci. One of these genes, the

Cited by 81 publications

References 26 publications

Pseudotypes of human T-cell leukemia virus types 1 and 2: neutralization by patients' sera.

Pseudotypes of human T-cell leukemia virus types 1 and 2: neutralization by patients' sera.

Reciprocal Inhibition of Mouse Leukemia Virus Infection by Fv-1 Allele Cell Extracts

A Transmissible Antigen Detected in Two Cell Lines Derived from Human Tumours

Contact Info

Product

Resources

About