Host responses within solid tumors: Non‐thymus‐derived specific cytotoxic cells within a murine mammary adenocarcinoma

Haskill, J. S.; Yamamura, Yuichi; Radov, L. A.

doi:10.1002/ijc.2910160512

Cited by 64 publications

(28 citation statements)

References 24 publications

(13 reference statements)

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“…Similar data were obtained when infiltration by host cells of in-vivogrowing tumour was studied (Haskill et al, 1975). The role of these subpopulations of syngeneic lymphoid cells in MTS growth inhibition is at present being investigated in an in vitro assay.…”

supporting

confidence: 63%

Multicellular tumour spheroids: a model for combined in vivo/in vitro assay of tumour immunity

Čulo¹,

Yuhas²,

Ladman³

1980

Br J Cancer

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Summary.-Multicellular tumour spheroids (MTS) from 4 mouse tumours (Line 1 lung carcinoma; a fibrosarcoma, FSA; a mammary carcinoma, MCa-11; and SV40-transformed fibroblasts, SV-A31) were injected into the abdominal cavity of normal, immunized or tumour-bearing syngeneic mice, recovered after 4-48 h, and their growth measured in vitro for 7-16 days. Both normal and immunized mice inhibited MTS growth, but there was no correlation between the two types of inhibition, suggesting that different immunological processes were involved. For example, the greatest inhibition by normal mice was seen for the weakly immunogenic MCa-11, and the highly immunogenic tumour, SV-A31, was only moderately inhibited. However, the summed inhibition of MTS growth in normal and sensitized hosts corresponded to the behaviour of tumours as s.c. transplants; i.e., was inversely related to the malignancy of the same tumours. The inhibition of MTS by mice bearing identical early tumours (FSA or MCa-11) was comparable to that in immunized mice. Histological sections of SV-A31 MTS in normal or immunized hosts revealed the infiltration of MTS by various types of host cells, mostly polymorphonuclears, macrophages and lymphocytes.

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supporting

confidence: 63%

Multicellular tumour spheroids: a model for combined in vivo/in vitro assay of tumour immunity

Čulo¹,

Yuhas²,

Ladman³

1980

Br J Cancer

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“…and has been adapt ed to tissue culture as previously described [14,30], A long-term culture line used in the present study produced progressively growing tumors in syngeneic hosts, when inoculated subcutaneously (s.c.) in the shoulder fat pad area, while similar tumors underwent spontane ous regression when the cells were inoculated s.c. in the belly. Origin and passage history of a fibrosarcoma SaD2 have been previously described 114).…”

Section: Tumor Cellsmentioning

confidence: 99%

“…In a syngeneic murine mammary adenocarcinoma T1699 growing in DBA/2J female mice, we have pre viously demonstrated that in situ effector cells [15,16) are specifically armed monocytes [14][15][16] and also that the serum from tumor-bearing hosts contained a non antibody factor(s) which could transfer specific anti tumor immunity to normal recipients. Such host immu nity provided by passive transfer of serum depended on some host cellular components [30].…”

Section: Introductionmentioning

confidence: 99%

Immunological Responses to a Murine Mammary Adenocarcinoma

Yamamura¹

1980

Oncology

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Serum from DBA/2J mice bearing a syngeneic T1699 mammary adenocarcinoma contains not only a specific antitumor antibody but also another factor(s) which arm normal monocytes in vitro and transform them into specific killer cells. Arming of normal monocytes by the factor(s) was inhibited by cytochalasin B and dactinomycin but not by mitomycin C. The ADCC activity of monocytes was at least partially resistant to the action of dactinomycin but cytotoxicity of armed effectors was totally blocked by the same drug. Effective cytolysis by armed monocytes required at least 5–6 h of continuous contact between the target and effector cells; and cytotoxicity could be reversed by cytochalasin B during this period.

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“…HUMAN and transplantable animal tumours have been shown to contain a variety of host cells such as macrophages, lymphocytes and " null " cells, amongst the neoplastic cells (Birbeck and Carter, 1972; Eccles and Alexander, 1974; Evans, 1972;Gauci and Alexander, 1975;Haskill, Yamamura and Radov, 1975;Mansell et al, 1975;Russell, Doe and Cockrane, 1976;Szymaniec and James, 1976;van Loveren and den Wood, Gillespie and Barth, 1975). The reason for their presence, and the mechanism attracting them to the tumour, are not clear.…”

mentioning

confidence: 99%

Effect of X-irradiation on host-cell infiltration and growth of a murine fibrosarcoma

Evans¹

1977

Br J Cancer

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Summary.-Whole body X-irradiation (400 rad) of C57BL mice, either before or after i.m. implantation of the syngeneic fibrosarcoma, FS6, influenced both the growth of the tumours and their cellular composition, particularly their macrophage content. Pre-irradiation resulted in slower initial growth of tumours, and a concomitant lack of host -cell infiltration, but when tumours began to grow at a rate parallel to controls infiltration by host cells was demonstrable. Similarly, irradiation of the tumourbearing host resulted in a temporary cessation of growth, and a decrease in the macrophage content, which did not return to control levels for 2-3 weeks after irradiation. (Evans, 1972). Moreover, for a given tumour, the level was also shown to be relatively constant, both during the growth of the tumour and when it was transplanted to a syngeneic recipient.In an attempt to further our understanding of the mechanisms which affect tumour growth and the cellular composition of the tumour, mice were exposed to whole-body irradiation either before the C57BL fibrosarcoma, FS6, was implanted, or after the tumour had been growing for several days. MATERIALS AND METHODSMice.-Eight 10-week-old male C57BL mice, weighing 18-24 g, were used for growth of the syngeneic FS6 tumour, which was benzpyrene-induced in 1969 and maintained by regular passage in syngeneic mice by injecting cells into the musculature of the right hind leg. This tumour is highly immunogenic, as shown either by concomitant immunity techniques or by rejection of large numbers of challenge tumour cells after surgical excision of the primary tumour.Tumnour.-Cell suspensions from intramuscular FS6 tumours were prepared by a modification of the technique fully described elsewhere (Evans, 1972). Trypsin was omitted from the enzyme solution and the percentage of collagenase was increased to 0 2%. Deoxyribonuclease (Sigma) was also incorporated at 1 ,ug/ml. The reason for omitting trypsin was that separation of adherent, non-neoplastic cells (see below) was achieved more readily if the FS6 tumour cells had not been previously exposed to trypsin. In all experiments 106 tumour cells were injected i.m. This size of inoculum gave a

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Host responses within solid tumors: Non‐thymus‐derived specific cytotoxic cells within a murine mammary adenocarcinoma

Cited by 64 publications

References 24 publications

Multicellular tumour spheroids: a model for combined in vivo/in vitro assay of tumour immunity

Multicellular tumour spheroids: a model for combined in vivo/in vitro assay of tumour immunity

Immunological Responses to a Murine Mammary Adenocarcinoma

Effect of X-irradiation on host-cell infiltration and growth of a murine fibrosarcoma

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