Host Reactions to Biomaterials and Their Evaluation

Anderson, James M.; Gristina, Anthony G.; Hanson, Stephen R.; Harker, Laurence A.; Johnson, Richard J.; Merritt, Katharine; Naylor, Paul T.; Schöen, Frederick J.

doi:10.1016/b978-0-08-050014-0.50009-2

Cited by 66 publications

(18 citation statements)

References 79 publications

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“…7,8 These transplants, however, are rather limited in that they are only 1–2 cell layers thick, which can severely limit function depending on tissue type, and that the material component may elicit a host response upon implantation. 9 …”

Section: Introductionmentioning

confidence: 99%

Electrospun poly(N-isopropyl acrylamide)/poly(caprolactone) fibers for the generation of anisotropic cell sheets

et al. 2017

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Cell alignment in muscle, nervous tissue, and cartilage is requisite for proper tissue function; however, cell sheeting techniques using the thermosensitive polymer poly(N-isopropyl acrylamide) (PNIPAAm) can only produce anisotropic cell sheets with delicate and resource-intensive modifications. We hypothesized that electrospinning, a relatively simple and inexpensive technique to generate aligned polymer fibers, could be used to fabricate anisotropic PNIPAAm and poly(caprolactone) (PCL) blended surfaces that both support cell viability and permit cell sheet detachment via PNIPAAm dissolution. Aligned electrospun PNIPAAm/PCL fibers (0%, 25%, 50%, 75%, 90%, and 100% PNIPAAm) were electrospun and characterized. Fibers ranged in diameter from 1–3 μm, and all fibers had an orientation index greater than 0.65. Fourier transform infrared spectroscopy was used to confirm the relative content of PNIPAAm and PCL. For advancing water contact angle and mass loss studies, only high PNIPAAm-content fibers (75% and greater) exhibited, temperature-dependent properties like 100% PNIPAAm fibers, whereas 25% and 50% PNIPAAm fibers behaved similarly to PCL-only fibers. 3T3 fibroblasts seeded on all PNIPAAm/PCL fibers had high cell viability and spreading except for the 100% PNIPAAm fibers. Cell sheet detachment by incubation with cold medium was successful only for 90% PNIPAAm fibers, which had a sufficient amount of PCL to allow cell attachment and spreading but not enough to prevent detachment upon PNIPAAm dissolution. This study demonstrates the feasibility of using anisotropic electrospun PNIPAAm/PCL fibers to generate aligned cell sheets that can potentially better recapitulate anisotropic architecture to achieve proper tissue function.

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Section: Introductionmentioning

confidence: 99%

Electrospun poly(N-isopropyl acrylamide)/poly(caprolactone) fibers for the generation of anisotropic cell sheets

et al. 2017

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“…12,13 As a result, the implant is isolated from the rest of the body, thus leading to a lack of biointegration and ultimately failure. 14 Therefore, it is essential to strategically engineer biomaterials that promote or inhibit specific phenotypes of macrophages to restore a balance in their behaviour.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory effects of octadecylamine-functionalized nanodiamond on primary human macrophages

et al. 2017

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Chronic inflammatory disorders such as rheumatoid arthritis are characterized by excessive pro-inflammatory or “M1” activation of macrophages, the primary cells of the innate immune system. Current treatments include delivery of glucocorticoids (e.g. dexamethasone – Dex), which reduce pro-inflammatory M1 behaviour in macrophages. However, these treatments have many off-target effects on cells other than macrophages, resulting in broad immunosuppression. To limit such side effects, drug-incorporated nano- and microparticles may be used to selectively target macrophages via phagocytosis, because of their roles as highly effective phagocytes in the body. In this study, surface-modified nanodiamond (ND) was explored as a platform for the delivery of dexamethasone to macrophages because of ND’s rich surface chemistry, which contributes to ND’s high potential as a versatile drug delivery platform. After finding that octadecylamine-functionalized nanodiamond (ND-ODA) enhanced adsorption of Dex compared to carboxylated ND, the effects of Dex, ND-ODA, and Dex-adsorbed ND-ODA on primary human macrophage gene expression were characterized. Surprisingly, even in the absence of Dex, ND-ODA had strong anti-inflammatory effects, as determined by multiplex gene expression via NanoString and by protein secretion analysis via ELISA. ND-ODA also inhibited expression of M2a markers yet increased the expression of M2c markers and phagocytic receptors. Interestingly, the adsorption of Dex to ND-ODA further increased some anti-inflammatory effects, but abrogated the effect on phagocytic receptors, compared to its individual components. Overall, the ability of ND-ODA to promote anti-inflammatory and pro-phagocytic behaviour in macrophages, even in the absence of loaded drugs, suggests its potential for use as an anti-inflammatory therapeutic to directly target macrophages through phagocytosis.

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“…A connective tissue (CT) capsule forms around a bio-inert implant normally within a few days. The thickness of the capsule depends on the implant material and the stage of healing (Andersson 1996). Epithelial lining partly separates the implanted devices that penetrate the skin or oral mucosa from the normal soft tissue components in that area (Winter 1974).…”

mentioning

confidence: 99%

Peri‐implant tissue response to TiO₂ surface modified implants

Rossi

Tirri

Paldan

et al. 2008

Clinical Oral Implants Res

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Host Reactions to Biomaterials and Their Evaluation

Cited by 66 publications

References 79 publications

Electrospun poly(N-isopropyl acrylamide)/poly(caprolactone) fibers for the generation of anisotropic cell sheets

Electrospun poly(N-isopropyl acrylamide)/poly(caprolactone) fibers for the generation of anisotropic cell sheets

Anti-inflammatory effects of octadecylamine-functionalized nanodiamond on primary human macrophages

Peri‐implant tissue response to TiO₂ surface modified implants

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Host Reactions to Biomaterials and Their Evaluation

Cited by 66 publications

References 79 publications

Electrospun poly(N-isopropyl acrylamide)/poly(caprolactone) fibers for the generation of anisotropic cell sheets

Electrospun poly(N-isopropyl acrylamide)/poly(caprolactone) fibers for the generation of anisotropic cell sheets

Anti-inflammatory effects of octadecylamine-functionalized nanodiamond on primary human macrophages

Peri‐implant tissue response to TiO2 surface modified implants

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Product

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Peri‐implant tissue response to TiO₂ surface modified implants