Host range deletion mutant of vaccinia virus defective in human cells

Drillien, Robert; Koehren, F.; Kirn, A.

doi:10.1016/0042-6822(81)90351-2

Cited by 90 publications

(61 citation statements)

References 22 publications

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“…The isolation of VV host range mutants which are unable to multiply in some cell types has suggested that viral functions are involved in determining such a wide host range. One mutant isolated in our laboratory from the Copenhagen strain, designated VV hr, has 18 kbp deleted near the left end of the genome (Drillien et al, 1981). Similar mutants have been isolated by multiple passages of the CVA strain on chick embryo fibroblasts (Mayr et al, 1975;Altenburger et al, 1989;Meyer et al, 1991) or by directed deletion of VV sequences using recombinant plasmids (Perkus et al, 1991).…”

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confidence: 99%

Detection of a protein encoded by the vaccinia virus C7L open reading frame and study of its effect on virus multiplication in different cell lines

Oguiura

Spehner

Drillien

1993

Journal of General Virology

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Vaccinia virus encodes several proteins, the activity of which is essential for multiplication in different cell types. Both the C7L and K1L open reading frames (ORFs) have been characterized as viral determinants for multiplication in human cells. To confirm and extend these findings we inserted the C7L ORF into the genome of a mutant virus unable to multiply in human cells and showed that this virus recovered its ability to replicate. Deletion of C7L from a wild-type viral genome did not adversely affect virus multiplication in human cells but it did reduce replication in hamster Dede ceils. When both C7L and K1L were deleted from the vaccinia virus genome only poor or no viral yields were obtained from various human cell lines. Recombinant viruses were also constructed to facilitate the study of C7L protein synthesis during infection. One virus in which the lacZ ORF was fused downstream and in-frame with the C7L ORF enabled us to characterize the C7L protein as an early gene product. Another recombinant virus was constructed so that the carboxy terminus of the C7L ORF product contained an additional 28 amino acids from the carboxy terminus of K1L. Tagging of C7L in this way allowed us to detect the fusion protein by immunoprecipitation with antibodies against the K1L protein. Furthermore, the hybrid protein retained its biological properties. The recombinant viruses constructed in this work should be useful for studies of the molecular basis of the activity of viral host range proteins.

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confidence: 99%

Detection of a protein encoded by the vaccinia virus C7L open reading frame and study of its effect on virus multiplication in different cell lines

Oguiura

Spehner

Drillien

1993

Journal of General Virology

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“…This is in contrast to the near-terminal regions of poxvirus genomes which are quite dissimilar with limited conservation between members of a given genus, but little conservation between genera Upton et al, 1987a). There is evidence that located within these regions is much of the genetic material involved in pathogenicity and host range specificity (Bloom et al, 1991 ;Drillien et al, 1981 ;Gillard et al, 1986;Spehner et al, 1988). The complete genome of vaccinia virus has been sequenced and near-terminal-encoded products include viral homologues of epidermal growth factor (Blomquist et al, 1984;Brown et al, 1985;Reisner, 1985), serine protease inhibitors (serpins) , protein kinase Traktman et al, 1989), a dUTPase-like gene (McGeoch, 1990;Slabaugh & Roseman, 1989) and complement control protein analogues (Kotwal & Moss, 1988a;Takahashi-Nishimaki et al, 1991).…”

Section: Introductionmentioning

confidence: 89%

Nucleotide sequence analysis of a unique near-terminal region of the tumorigenic poxvirus, Shope fibroma virus

Massung

McFadden

Moyer

1992

Journal of General Virology

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Shope fibroma virus (SFV), a tumorigenic poxvirus, has a DNA genome of approximately 160 kb. Previous DNA sequence analysis of SFV has been mainly limited to the terminal inverted repetitions (about 12 kb at each end of the genome) and immediately adjacent regions. We have sequenced a 4 kb fragment located approximately 20 kb from the right-terminal hairpin. Within this region three complete and two partial open reading frames (ORFs) have been identified. Each of the putative polypeptides has sequence similarity to one or more previously identified poxvirus or cellular proteins, with homology to protein kinases, erythrocyte ankyrin and a vaccinia virus virulencerelated protein (ORF NIL). The potential significance of these gene products with regard to the phenotype of SFV is discussed.

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“…It has been shown previously that the production of epitopes can differ in different cell types (51). Vaccinia viruses have a very broad replication capability in different human cells (52), but because MVA is replication defective in human cells (53), the primary target cells after intradermal application might be skin fibroblasts (53) and to some degree skin dendritic cells (53,54). In contrast, the immunodeficiency viruses replicate mostly in CD4 ϩ cells (55)(56)(57)(58)(59).…”

Section: Discussionmentioning

confidence: 99%

Differences Between T Cell Epitopes Recognized After Immunization and After Infection

Vogel

Horton

Fuller

et al. 2002

The Journal of Immunology

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Evidence suggests that cellular immune responses play a crucial role in the control of HIV and SIV replication in infected individuals. Several vaccine strategies have therefore targeted these CD8+ and CD4+ responses. Whether vaccination induces the same repertoire of responses seen after infection is, however, a key unanswered question in HIV vaccine development. We therefore compared the epitope specificity induced by vaccination to that present postchallenge in the peripheral blood. Intracellular cytokine staining of PBMC stimulated with overlapping 15/20-mer peptides spanning the proteins of SIV were measured after DNA/modified vaccinia Ankara vaccination of eight rhesus macaques. Lymphocytes from 8 animals recognized a total of 39 CD8 epitopes and 41 CD4 epitopes encoded by the vaccine. T cell responses were again monitored after challenge with SIVmac239 to investigate the evolution of these responses. Only 57% of all CD8+ T cell responses and 19% of all CD4+ T cell responses present after vaccination were recalled after infection as measured in the peripheral blood. Interestingly, 29 new CD8 epitopes and 5 new CD4 epitopes were recognized by PBMC in the acute phase. These new epitopes were not detected after vaccination, and only some of them were maintained in the chronic phase (33% of CD8 and no CD4 responses). Additionally, 24 new CD8 epitopes and 7 new CD4 epitopes were recognized by PBMC in the chronic phase of infection. The repertoire of the immune response detected in the peripheral blood after immunization substantially differed from the immune response detected in the peripheral blood after infection.

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Host range deletion mutant of vaccinia virus defective in human cells

Cited by 90 publications

References 22 publications

Detection of a protein encoded by the vaccinia virus C7L open reading frame and study of its effect on virus multiplication in different cell lines

Detection of a protein encoded by the vaccinia virus C7L open reading frame and study of its effect on virus multiplication in different cell lines

Nucleotide sequence analysis of a unique near-terminal region of the tumorigenic poxvirus, Shope fibroma virus

Differences Between T Cell Epitopes Recognized After Immunization and After Infection

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