Host Range and Properties of the Pseudomonas aeruginosa R Factor R1822

Abstract

R1822, a plasmid specifying multiple drug resistances, has been transferred to a variety of species representative of related and unrelated genera. The host range of the plasmid includes Enterobacteriaceae, soil saprophytes, Neisseria perflava, and photosynthetic bacteria. With the acquisition of drug resistance(s), these strains became sensitive to a small, ribonuclease-sensitive bacteriophage, designated PRR1, isolated by enrichment from sewage. ' Received as PAT 2; spontaneous streptomycin-resistant mutant (1 mg/ml). d Received as PA0602; PAL(R1822) x PA0602. e Auxotrophic mutant of P71 isolated from milk by J. J. Jezeski, Univ. of Minnesota. ' Department of Microbiology, Univ. of Michigan. ' Spontaneous streptomycin-resistant mutant (1 mg/ml). hDepartment of Biochemistry, Univ. of Michigan. colonies from the primary isolation medium (always containing carbenicillin) to TGE agar medium containing tetracycline or neomycin at 50 ug/ml. Mating. R+ strains used in subsequent quantitative estimates of transfer frequencies were constructed by mixed inoculation of TGE broth medium I 773

The kilA operon of promiscuous plasmid RK2: the use of a transducing phage (λpklaA‐1) to determine the effects of the lethal klaA gene on Escherichia coli cells

“…Such precision probably accounts tor tha narrow host ranges of the large majority ot plasmids. However, some plasmids, like those of incompatibility group P (IncP), have a remarkably broad host range (Datta and Hedges, 1972;Olsen and Shipley. 1973; Thomas and Smith.…”

Analysis of copy number control elements in the region of the vegetative replication origin of the broad host range plasmid RK2.

“…Analysis of replication control in broad host-range plasmid RK2 (belonging to E. coli incompatibility group IncP; RK2 is similar or identical to RP4, RP1 and R68, Burkhardt et al, 1979) is of interest because RK2 is capable of regulated replication in a wide range of Gram negative bacterial species (Datta and Hedges, 1972;Olsen and Shipley, 1973;Beringer, 1974;Cho et al, 1975). An understanding of its copy number regulation may be useful for development of various types of broad host-range cloning vector.…”

The Primary Structure of the Coat Protein of the Broad‐host‐range Rna Bacteriophage Prr1

“…The RNA phage PRRl and the host on which its plaque-forming ability was optimal, Pseudomonas aeruginosa strain PAT904 Rev1 (RP1) [1,4] were both kind gifts of Dr Olsen (Michigan, U.S.A.).…”