Host Plant Specialization Governed by Facultative Symbiont

Tsuchida, Tsutomu; Koga, Ryuichi; Fukatsu, Takema

doi:10.1126/science.1094611

Cited by 479 publications

(458 citation statements)

References 4 publications

Supporting

Mentioning

438

Contrasting

Unclassified

Order By: Relevance

“…However, upon Regiella transfection, their survival and reproduction on the non-host plant are slightly but significantly improved. Considering that the Regiella strain used in Table 1 this study improves fecundity of A. pisum specifically on clover [12], these results can be interpreted as the transfer of an ecological trait, viability on clover, from A. pisum to M. crassicauda by symbiont transfection. Recently, a number of studies have demonstrated environment-dependent fitness advantages conferred by facultative symbionts, and the possibility has been argued that such ecological traits might be moving around between different species in association with horizontal symbiont transfers (reviewed in [2]).…”

Section: Resultsmentioning

confidence: 99%

“…Diagnostic PCR and 16S rRNA gene cloning and sequencing confirmed that these strains harboured the obligate endosymbiont B. aphidicola but no facultative symbionts. A Regiella-infected strain, TUt, of pea aphid A. pisum was used as a donor of Regiella, wherein Regiella infection caused enhanced host fecundity specifically on white clover Trifolium repens [12]. A Regiella-cured strain of the same genotype, TUt amp , was also used.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Interspecific symbiont transfection confers a novel ecological trait to the recipient insect

et al. 2010

Self Cite

View full text Add to dashboard Cite

In Japan, pea aphids Acyrthosiphon pisum mainly feed on vetch and clover, and many aphid clones produce more progeny on vetch than on clover. In this context, particular genotypes of the facultative symbiont Regiella insecticola enhance reproduction of infected pea aphids specifically on clover, thereby broadening the suitable food plant range of the insect. A species that is sympatric to A. pisum, vetch aphids Megoura crassicauda, are commonly found on vetch but not on clover. Laboratory rearing of M. crassicauda strains revealed active reproduction on vetch but substantially no reproduction on clover. Experimental transfection of Regiella from A. pisum to M. crassicauda by haemolymph injection established stable and heritable infection in the recipients, although no Regiella infection has been detected in natural populations of M. crassicauda. Different strains of Regiellatransfected M. crassicauda grew and reproduced on vetch, but exhibited lower fitness in comparison with corresponding uninfected aphid strains. Strikingly, the Regiella-transfected M. crassicauda exhibited improved survival and some reproduction on clover. These results suggest that Regiella has the potential to confer an ecological trait, adaptation to clover, on novel insect hosts, and also account for why Regiella is able to infect M. crassicauda but is scarcely found in these aphid populations.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Interspecific symbiont transfection confers a novel ecological trait to the recipient insect

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, evidence continues to mount demonstrating the outcome of aphid‐host plant interactions critically depends on microbial associations (Tsuchida et al. 2004; Ferrari et al. 2007; Francis et al.…”

Section: Introductionmentioning

confidence: 99%

Stress‐induced changes in abundance differ among obligate and facultative endosymbionts of the soybean aphid

Enders

Miller

2016

Ecology and Evolution

View full text Add to dashboard Cite

Bacterial endosymbionts can drive evolutionary novelty by conferring adaptive benefits under adverse environmental conditions. Among aphid species there is growing evidence that symbionts influence tolerance to various forms of stress. However, the extent to which stress inflicted on the aphid host has cascading effects on symbiont community dynamics remains poorly understood. Here we simultaneously quantified the effect of host‐plant induced and xenobiotic stress on soybean aphid (Aphis glycines) fitness and relative abundance of its three bacterial symbionts. Exposure to soybean defensive stress (Rag1 gene) and a neurotoxic insecticide (thiamethoxam) substantially reduced aphid composite fitness (survival × reproduction) by 74 ± 10% and 92 ± 2%, respectively, which in turn induced distinctive changes in the endosymbiont microbiota. When challenged by host‐plant defenses a 1.4‐fold reduction in abundance of the obligate symbiont Buchnera was observed across four aphid clonal lines. Among facultative symbionts of Rag1‐stressed aphids, Wolbachia abundance increased twofold and Arsenophonus decreased 1.5‐fold. A similar pattern was observed under xenobiotic stress, with Buchnera and Arsenophonus titers decreasing (1.3‐fold) and Wolbachia increasing (1.5‐fold). Furthermore, variation in aphid virulence to Rag1 was positively correlated with changes in Arsenophonus titers, but not Wolbachia or Buchnera. A single Arsenophonus multi‐locus genotype was found among aphid clonal lines, indicating strain diversity is not primarily responsible for correlated host‐symbiont stress levels. Overall, our results demonstrate the nature of aphid symbioses can significantly affect the outcome of interactions under stress and suggests general changes in the microbiome can occur across multiple stress types.

show abstract

“…In addition, the pea aphid is associated with an obligatory endosymbiont, Buchnera aphidicola, which is found in specialized cells called bacteriocytes and provides its host with essential amino acids. The pea aphid also harbors several facultative symbionts whose distribution is strongly correlated with plant specialization of their hosts (Simon et al, 2003;Ferrari et al, 2012;Henry et al, 2013), and it has been posited that some of these symbionts could have a role in plant adaptation, although clear evidence is still lacking (Tsuchida et al, 2004;McLean et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Whole-genome re-sequencing of non-model organisms: lessons from unmapped reads

et al. 2014

View full text Add to dashboard Cite

Unmapped reads are often discarded from the analysis of whole-genome re-sequencing, but new biological information and insights can be uncovered through their analysis. In this paper, we investigate unmapped reads from the re-sequencing data of 33 pea aphid genomes from individuals specialized on different host plants. The unmapped reads for each individual were retrieved following mapping to the Acyrthosiphon pisum reference genome and its mitochondrial and symbiont genomes. These sets of unmapped reads were then cross-compared, revealing that a significant number of these unmapped sequences were conserved across individuals. Interestingly, sequences were most commonly shared between individuals adapted to the same host plant, suggesting that these sequences may contribute to the divergence between host plant specialized biotypes. Analysis of the contigs obtained from assembling the unmapped reads pooled by biotype allowed us to recover some divergent genomic regions previously excluded from analysis and to discover putative novel sequences of A. pisum and its symbionts. In conclusion, this study emphasizes the interest of the unmapped component of re-sequencing data sets and the potential loss of important information. We here propose strategies to aid the capture and interpretation of this information. Heredity (2015) 114, 494-501; doi:10.1038/hdy.2014.85; published online 1 October 2014 INTRODUCTION Next-generation sequencing and whole-genome re-sequencing is nowadays commonly used to identify genomic variants that underlie phenotypic variations, genetic diseases, adaptation or speciation in natural populations. Typically, the reads are mapped against a reference genome, and the genotypes (that is, single-nucleotide polymorphism (SNP) and structural variant calls) are based on these mapped reads (Altshuler et al., 2010;Nielsen et al., 2011). In addition to universal caveats regarding unknown insertions and/or genomic contamination, which can be overlooked in pure mapping approaches, non-model organisms may suffer from the poor quality of the nuclear reference genome and incomplete symbiont or organellar genomes. Moreover, mapping is constrained by the level of divergence between the reads and the available reference sequence (Sousa and Hey, 2013). The resulting ascertainment bias could be problematic, especially when studying adaptation or speciation processes, as genomic regions of interest are expected to display important levels of divergence. These different issues produce a non-negligible fraction of unmapped reads, whose sequences are generally disregarded in favor of the mapped reads in the subsequent steps of the analysis, despite potentially containing useful information. This study offers one strategy for mining the unmapped reads in order to extract the relevant biological knowledge, leading to advice and recommendations for other re-sequencing projects.We investigated this question in the context of a large-scale resequencing project on the pea aphid species complex. The pea aphid

show abstract

Host Plant Specialization Governed by Facultative Symbiont

Cited by 479 publications

References 4 publications

Interspecific symbiont transfection confers a novel ecological trait to the recipient insect

Interspecific symbiont transfection confers a novel ecological trait to the recipient insect

Stress‐induced changes in abundance differ among obligate and facultative endosymbionts of the soybean aphid

Whole-genome re-sequencing of non-model organisms: lessons from unmapped reads

Contact Info

Product

Resources

About