“…MLST analysis of Wolbachia and successful horizontal transfer of Wolbachia by microinjection have also provided evidence for horizontal transmission (Xi et al, 2005(Xi et al, , 2006Li et al, 2017;Zheng et al, 2019). As recorded, horizontal transmission of Wolbachia could occur by many pathways, such as feeding on common plants (Sintupachee et al, 2006;Le Clec'h et al, 2013;Li et al, 2017;Sanaei et al, 2023), parasitic wasps (Ahmed et al, 2015;Brown and Lloyd, 2015;Goya et al, 2022), parasitic mites (Houck et al, 1991;Jaenike et al, 2007;Gehrer and Vorburger, 2012), hybridization (Jiang et al, 2018;Su et al, 2019), and predation (Goodacre et al, 2006;Wang et al, 2010;Su et al, 2019). Although interspecific horizontal transmission inefficiently occurs, Wolbachia horizontal transmission is found in many insects, including rice planthoppers (Zhang et al, 2013), wasps (Huigens et al, 2004;Goya et al, 2022;Zhou et al, 2022), fruit flies (Turelli et al, 2018), trypetids (Schuler et al, 2013(Schuler et al, ), psyllids 10.3389/fmicb.2023 Frontiers in Microbiology 03 frontiersin.org (Serbina et al, 2022), moths (Ahmed et al, 2016), ladybirds (Shaikevich and Romanov, 2023), mosquitoes (Shaikevich et al, 2019), mites (Su et al, 2019), butterflies (Ahmed et al, 2016;Zhao et al, 2021) and so forth.…”