Host effect on genetic variation of Marssonina brunnea pathogenic to poplars

Zhao, Han; Yin, Tongming; Li, C. D.; Huang, Minren; Wu, Rui

doi:10.1007/s001220050081

Cited by 26 publications

(21 citation statements)

References 18 publications

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“…multigermtubi (MULT), infects susceptible hosts strictly limited in sections Aigeiros and Tacamahaca. Cross-infectivity on hosts among the sections mentioned above does not happen on each forma specialis in the field (Spiers, 1989;Han et al, 2000). However, previous reports showed that the two formae speciales shared a very highly similar infection process in respective susceptible hosts living a hemibiotrophic lifestyle (Zhang et al, 2018a,b).…”

Section: Introductionmentioning

confidence: 91%

“…It often severely breaks out in poplar plantations all over the world recently and threatens poplar crops for the future (Erickson et al, 2004;Zhu et al, 2012;Call and St. Clair, 2017;Zhang et al, 2018a). The population of the pathogen has been verified to be differentiated into two host-adapted formae speciales in the field based on host ranges, pathogenicity, and phylogenetic nature (Spiers, 1984;Han et al, 1998aHan et al, , 2000Sun et al, 2015;Call and St. Clair, 2017;Zhang et al, 2018b). In China (Han et al, 1998a(Han et al, ,b, 2000Sun et al, 2015;Zhang et al, 2018b), one forma specialis, M. brunnea f. sp.…”

Section: Introductionmentioning

confidence: 99%

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Distinctive Gene Expression Profiles and Effectors Consistent With Host Specificity in Two Formae Speciales of Marssonina brunnea

Ren

Yan

et al. 2020

Front. Microbiol.

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The knowledge on the host specificity of a pathogen underlying an interaction is becoming an urgent necessity for global warming. In this study, the gene expression profiles and the roles of effectors in host specificity were integrally characterized with two formae speciales, multigermtubi and monogermtubi, of a hemibiotrophic pathogen Marssonina brunnea when they were infecting respective susceptible poplar hosts. With a functional genome comparison referring to a de novo transcriptome of M. brunnea and Pathogen-Host Interaction database functional annotations, the multigermtubi strain showed abundant and significant differentially expressed unigenes (DEGs) (more than 40%) in colonizing the initial invasion stage and in the necrotrophic stage. The monogermtubi strain induced less than 10% of DEGs in the initial invasion stage but which abruptly increased to more than 80% DEGs in the necrotrophic stage. Both strains induced the least DEGs in the biotrophic stage compared to the initial invasion and necrotrophic stages. The orthologs of the effector genes Ecp6, PemG1, XEG1, ACE1, and Mg3LysM were exclusively induced by one of the two formae speciales depending on the infection stages. Some unigenes homologous to carbohydrate lytic enzyme genes, especially pectate lyases, were notably induced with multigermtubi forma specialis infection but not expressed in the monogermtubi forma specialis at an earlier infection stage. The extraordinary differences in the functional genome level between the two formae speciales of M. brunnea could be fundamental to exploring their host specificity determinant and evolution. This study also firstly provided the fungal transcriptome of the monogermtubi forma specialis for M. brunnea.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Distinctive Gene Expression Profiles and Effectors Consistent With Host Specificity in Two Formae Speciales of Marssonina brunnea

Ren

Yan

et al. 2020

Front. Microbiol.

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“…As previously reported, the sexual state of M. brunnea was not found in China and New Zealand, and genomic analysis showed that this pathogen might lack the capacity to perform sexual reproduction in China (He & Yang, ; Han, Li, & Huang, ; Zhu et al., ). M. brunnea infects various poplar species (Han, Yin, Li, Huang, & Wu, ; Spiers, ) and has often caused outbreaks in various countries in Europe (Newcombe & Callan, ; Spiers, ).…”

Section: Introductionmentioning

confidence: 99%

“…Marssonina brunnea epidemics often occur in poplar plantations in the northeast, northern and southern regions of China (Han et al., ; He & Yang, ). Based on its extensive area of infection, analysts have suggested that the pathogenicity of the fungal species might have changed.…”

Section: Introductionmentioning

confidence: 99%

Genomewide identification and development of microsatellite markers for Marssonina brunnea and their applications in two populations

Zhang

Yan

2018

Forest Pathology

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Summary Marssonina brunnea is an important pathogen that causes Marssonina leaf spot disease of poplar (MLDP) in various poplar species. Resistance breeding is considered as the main method for preventing this disease and requires information on genetic diversity and population structure. However, molecular markers that may be utilized in the identification of this fungus are limited. This study investigated the distribution of microsatellites in the M. brunnea genome. A total of 15,356 microsatellite markers (excluding mononucleotide repeats) were isolated from 50.1 Mb of genomic sequence. Eight M. brunnea isolates were evaluated in terms of 102 loci, followed by the selection of markers that could be utilized in investigating the population structure of 47 M. brunnea isolates from two populations. Twenty‐four polymorphic microsatellite markers were developed for M. brunnea. The number of alleles per locus ranged from two to eight (average: 3.75). The polymorphism information content (PIC) of these loci ranged from 0.0408 to 0.6492. The average Shannon's information index of these loci in the two populations was 0.3819 and 0.5351, respectively. Using these markers, M. brunnea isolates were mainly divided into two distinct clusters based on the relatedness of the sampling sites. These results indicate that the selected markers could be effectively utilized in investigating the population genetic structure of M. brunnea. This is the first report on microsatellite markers in M. brunnea.

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“…M. brunnea displays a high degree of host specialization within the Populus genus. Based on host ranges, strains of M. brunnea have been classified into different specialized forms 3. However, M. brunnea is a non‐obligate pathogen and can be cultured in vitro on simple media 4.…”

Section: Introductionmentioning

confidence: 99%

Identifying secreted proteins of Marssonina brunnea by degenerate PCR

et al. 2010

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Marssonina brunnea is an important fungal pathogen of the Populus genus. To further our understanding of the pathogenesis of M. brunnea, we initiated a proteome-level study of the fungal secretome. Using de novo peptide sequencing by MS/MS, we obtained peptide sequences for 32 protein spots. Four proteins were identified by sequence homology to conserved proteins in public databases using MS-driven BLAST. To identify additional protein spots, we combined a degenerate PCR method, based on the Consensus-DEgenerate Hybrid Oligonucleotide Primer (CODEHOP) method, and a rapid amplification of cDNA ends method to clone the full-length cDNA fragments encoding the proteins identified in the gel. Using this method, we cloned the full-length cDNA fragments encoding 11 M. brunnea-specific proteins. This method provides an efficient approach to identification of species-specific proteins of non-sequenced organisms. Furthermore, we analyzed the expression patterns of these genes during infection. We found that most of the identified secreted proteins could be induced in artificial medium after hyphae entered poplar apoplast spaces. We propose that for the host-specialized M. brunnea, the elongation of hyphae has evolved closely with the secretion of apoplastic proteins.

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Host effect on genetic variation of Marssonina brunnea pathogenic to poplars

Cited by 26 publications

References 18 publications

Distinctive Gene Expression Profiles and Effectors Consistent With Host Specificity in Two Formae Speciales of Marssonina brunnea

Distinctive Gene Expression Profiles and Effectors Consistent With Host Specificity in Two Formae Speciales of Marssonina brunnea

Genomewide identification and development of microsatellite markers for Marssonina brunnea and their applications in two populations

Identifying secreted proteins of Marssonina brunnea by degenerate PCR

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