Host-dependent Suppression of Temperature-sensitive Mutations in Tobacco Mosaic Virus Transport Gene

Abstract

SUMMARYTobacco mosaic virus (TMV) mutants Lsl and Ni2519, temperature-sensitive (ts) in transport function in tobacco plants, were able to spread at high temperature (33 °C) in Amaranthus caudatus L. plants. On the other hand, TMV ts coat protein mutants retained the ts phenotype in both host plants. The ability of Lsl and Ni2519 to spread systemically in A. caudatus at high temperature is probably due to the functional stabilization of the transport proteins by a factor(s) provided by the host plant. In accordance with this, Lsl complemented the transport function of cucumber green mottle mosaic tobamovirus and red clover mottle comovirus; they acquired the ability to spread systemically at 33 °C in the A. caudatus plants preinfected with Lsl.

Movement of tomato mosaic virus strains inGomphrena globosaas related to temperature sensitivity and plant resistance

“…Previous reports showed that LS1 was TS in all plants tested except A. caudatus, also a plant with C4 metabolism (9,16). To date, we have been unable to repeat these observations using a similar experimental protocol (C.J.…”

“…French, unpublished data); the reasons for this discrepancy are under investigation. Therefore, no generalizations can be made on an association of C4 metabolism with putative host stabilizing factors at nonpermissive temperatures (16).…”

“…Can Among ToMV strains, L and LSI differ in a single amino acid substitution in the 30-kDa movement protein (Pro'53 to Ser) and this mutation accounts for temperature sensitivity (TS) in virus spread in various C3 plants (2,15). In contrast, LS, was reported to be temperature resistant (TR) in Amaranthus caudatus L., a plant with C4 metabolism; both L and LSI moved within the inoculated leaf with equal facility at 22 and 32°C (16).…”

Interactions of soybean mosaic virus with susceptible and resistant lines of soybean

“…It occurs when the gene product of both viruses is a multimeric protein and each virus is defective in different domains of the functional viral protein (Fincham, 1966). Complementation in mixed infections of plant viruses has been suggested (Dodds and Hamilton, 1976;Mushegian et al, 1989), but has not been extensively used with most RNA plant viruses for genetic analysis.…”