Host Defence Mechanisms Against Japanese Encephalitis Virus Infection in Mice

Mathur, Asha; Arora, K. L.; Chaturvedi, U. C.

doi:10.1099/0022-1317-64-4-805

Cited by 64 publications

(43 citation statements)

References 21 publications

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“…In addition, i.c. challenge experiments with nude mice (Miura et al, 1990;Lad et al, 1993) and passively transferred virusimmune spleen cells (Mathur et al, 1983) suggest a T cell involvement in protection against i.c. challenge with JEV.…”

Section: Discussionmentioning

confidence: 99%

Protection of adult but not newborn mice against lethal intracerebral challenge with Japanese encephalitis virus by adoptively transferred virus-specific cytotoxic T lymphocytes: requirement for L3T4+ T cells

Ravi

Manjunath

1996

Journal of General Virology

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Section: Discussionmentioning

confidence: 99%

Protection of adult but not newborn mice against lethal intracerebral challenge with Japanese encephalitis virus by adoptively transferred virus-specific cytotoxic T lymphocytes: requirement for L3T4+ T cells

Ravi

Manjunath

1996

Journal of General Virology

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“…JEV-specific humoral (30) and cellular (31,32) immune responses have been suggested to be responsible for protective immunity against JEV infection. Among the various structural and nonstructural proteins of JEV, the E protein appears to play the most important role in inducing protective immunity (24).…”

Section: Figurementioning

confidence: 99%

Suppression of Immune Response and Protective Immunity to a Japanese Encephalitis Virus DNA Vaccine by Coadministration of an IL-12-Expressing Plasmid

Chen

Pan

Huan

et al. 2001

The Journal of Immunology

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IL-12 plays a central role in both innate and acquired immunity and has been demonstrated to potentiate the protective immunity in several experimental vaccines. However, in this study, we show that IL-12 can be detrimental to the immune responses elicited by a plasmid DNA vaccine. Coadministration of the IL-12-expressing plasmid (pIL-12) significantly suppressed the protective immunity elicited by a plasmid DNA vaccine (pE) encoding the envelope protein of Japanese encephalitis virus. This suppressive effect was associated with marked reduction of specific T cell proliferation and Ab responses. A single dose of pIL-12 treatment with plasmid pE in initial priming resulted in significant immune suppression to subsequent pE booster immunization. The pIL-12-mediated immune suppression was dose dependent and evident only when the IL-12 gene was injected either before or coincident with the pE DNA vaccine. Finally, using IFN-γ gene-disrupted mice, we showed that the suppressive activity of the IL-12 plasmid was dependent upon endogenous production of IFN-γ. These results demonstrate that coexpression of the IL-12 gene can sometimes produce untoward effects to immune responses, and thus its application as a vaccine adjuvant should be carefully evaluated.

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“…The immune response to JEV infection in experimental animals as well as humans has been an active area of research in recent years (Desai et al, 1994(Desai et al, , 1995Mathur et al, 1983;Muralikrishna et al, 1996). Protection against JEV infection is probably determined largely by differential stimulation of CD4 + T cells.…”

Section: Discussionmentioning

confidence: 99%

T helper responses to Japanese encephalitis virus infection are dependent on the route of inoculation and the strain of mouse used

Ramakrishna

Ravi

Desai

et al. 2003

Journal of General Virology

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T helper cytokine and IgG subtype responses were studied in three strains of mice (C57BL/6J, Swiss albino, BALB/c; n=90 per strain) immunized with live Japanese encephalitis virus (JEV) by intraperitoneal (IP), subcutaneous (SC) and peroral (PO) routes. Lymphocytes obtained from the spleens of immunized and control mice were stimulated in vitro with JEV for 48 h and the supernatants were assayed for the presence of the cytokines IL-4 and IFN-c. JEV-specific IgG isotypes were also measured in the sera of immunized mice. T helper cytokine responses in mice immunized with JEV were found to be strain-and route-specific in the three species tested. Moreover, they were also dependent on the type of immunogen used (live vs killed virus), as well as the number of doses administered. C57BL/6J and BALB/c mice were more uniform in their T helper responses compared with the outbred Swiss albino mice and induced a good Th1 response (P<0?001). Among the three routes evaluated, the IP and SC routes consistently elicited a Th1 response compared with the PO route (P<0?001), where an initial Th2-type response reverted to a Th1 response after repeated immunization. Live JEV induced a Th1 response while the commercial killed vaccine induced a predominant Th2 profile.

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Host Defence Mechanisms Against Japanese Encephalitis Virus Infection in Mice

Cited by 64 publications

References 21 publications

Protection of adult but not newborn mice against lethal intracerebral challenge with Japanese encephalitis virus by adoptively transferred virus-specific cytotoxic T lymphocytes: requirement for L3T4+ T cells

Protection of adult but not newborn mice against lethal intracerebral challenge with Japanese encephalitis virus by adoptively transferred virus-specific cytotoxic T lymphocytes: requirement for L3T4+ T cells

Suppression of Immune Response and Protective Immunity to a Japanese Encephalitis Virus DNA Vaccine by Coadministration of an IL-12-Expressing Plasmid

T helper responses to Japanese encephalitis virus infection are dependent on the route of inoculation and the strain of mouse used

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