Host associated mixed probiotic bacteria induced digestive enzymes in the gut of tiger shrimp Penaeus monodon

Abstract

The shrimp Penaeus monodon was used for the isolation of digestive enzyme producing host-associated probiotic bacteria. Gut was isolated from a healthy animal completely and morphologically different bacterial isolates were screened for the production of hydrolytic enzymes, such as, protease, amylase, lipase and cellulases. Based on their ability to produce enzymes, the potent probiotic bacteria were identified as Bacillus subtilis and B. licheniformis and these two were used for the preparation of probiotic diet for experimental trials. Probiotic diet was prepared by mixing the shrimp feed with 2 g probiotic/100 g artificial diet (F1), 4 g/100 g (F2), 6 g/100 g (F3), 8 g/100 g (F4) and 10 g/100 g (F5). Juvenile shrimp was fed with probiotic and control diet for a period of 7 weeks at 5 and 8% body weight for the first 3 and 7 weeks, respectively. After seven weeks, whole gut was dissected out and protease activity was estimated as 145 ± 12.3 U/g in control animal and increased as 710 ± 15.2 U/ g in F5 feed groups. Amylase activity was 139 ± 10.4 U/g in control and increased as 209 ± 13. 3 U/g in F5 group. Cellulase activities were 171 ± 9.3 in F5 groups and the control group showed only 102 ± 12.4 U/g. Lipase activity was 78 ± 3 U/g in F1 groups and it increased as 85 ± 5 U/g in F3 groups. These findings indicate the potential of host-associated bacteria to enhance the production of enzymes in the gut of juvenile P. monodon .

PERFORMANCE OF PACIFIC WHITE SHRIMP FED PROBIOTICS <i>Lactobacillus plantarum</i> AND <i>Bacillus</i> spp. IN A BIOFLOC SYSTEM

“…To obtain a better effect of probiotics, several studies using combinations of lactic bacteria and Bacillus spp. reported improvements in shrimp performance, in some cases with even better results than those obtained with the strains used separately, indicating a synergistic effect (Kumar et al, 2014;Sánchez-Ortiz et al, 2016;Hostins et al, 2017;Xie et al, 2019;Wang et al, 2019;Wang et al, 2020). However, contrary to what was expected, the combined use of L. plantarum and Bacillus spp.…”

“…Lactic bacteria and Bacillus are the most used bacterial strains in aquaculture. Several studies report positive results with the use of these strains (endogenous or exogenous, mono, or multispecies) on growth, survival and/ or feed conversion ratio in marine shrimp (Kumar et al, 2014;Sánchez-Ortiz et al, 2016;Franco et al, 2017;Hostins et al, 2017;Zheng et al, 2017;Nguyen et al, 2018;Kewcharoen and Srisapoome, 2019;Xie et al, 2019;Zuo et al, 2019;Wang et al, 2020;Won et al, 2020).…”

Biofilm producing indigenous bacteria isolated from municipal sludge and their nutrient removal ability in moving bed biofilm reactor from the wastewater

“…Total protein content of the sample was evaluated as described previously ( Lowry et al, 1951 ). Enzyme activity was expressed as U/mg protein ( Wang et al, 2020 ). From these 18 bacterial strains only two bacteria were selected for characterization studies.…”

Draft Genome Sequence of Microbacterium sp. Strain KKR3/1, an Antimicrobial-Substance-Producing Strain Isolated from Banana Shrimp (Atyopsis moluccensis)

“…The gut microflora of the shrimp Atyopsis moluccensis is of particular interest due to the unique feeding strategy of this organism. The absence of a number of its own digestive enzymes suggests the presence of a large microbial diversity in the composition of its gut microbiome ( 2 , 3 ).…”