Host‐associated
 Bacillus siamensis
 and
 Lactococcus petauri
 improved growth performance, innate immunity, antioxidant activity and ammonia tolerance in juvenile Japanese seabass (
 Lateolabrax japonicus
 )

Yang, Hongling; Liu, Ziyan; Jian, Jiu-Ting; Ye, Jidan; Sun, Yun‐Zhang

doi:10.1111/anu.13399

Cited by 18 publications

(9 citation statements)

References 55 publications

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“…In the present study, the blood cortisol levels declined in the supplemented groups compared to control after ammonia stress, suggesting a stress ameliorating function for RS and the probiotic. Similarly, the ameliorating effects of probiotics (Salama et al, 2016;Yang et al, 2021;Gopi et al, 2022) and medicinal herbs (Chen et al, 2016;Esmaeili et al, 2017;Zuo et al, 2022) on ammonia stress has been also reported in many studies. The probiotic and herb related stress mitigating mechanisms are not yet recognized in fish.…”

Section: Discussionmentioning

confidence: 86%

Rosmarinic acid alone or in combination with Lactobacillus rhamnosus ameliorated resistance to ammonia stress in the rainbow trout, Oncorhynchus mykiss: growth, immunity, antioxidant defense and liver functions

Hajirezaee

Khanjani

2023

Annals of Animal Science

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Rosmarinic acid (RS) and Lactobacillus rhamnosus (LR) were added singularly or in combination to rainbow trout (Oncorhynchus mykiss) diets to test their efficacy in the protection against ammonia stress. Fish (31.4±0.6 g) were randomly allocated to six groups in three replicates, as follows: T1: basic food as control, T2: LR with a concentration of 1.5 × 108 CFU/g, T3: LR with a concentration of 3 × 108 CFU/g, T4: 1 g RS/kg, T5: 3 g RS/kg, and T6: 1.5 × 108 CFU/g LR + 1 g RS/kg and T7: 3 × 108 CFU/g LR + 3 g RS/kg. After 60 days feeding, fish exposed to 0ammonia stress. After the feeding period, the supplemented fish had the highest final body weight (FW), weight gain (WG), and specific growth rate (SGR), and the lowest feed conversion ratio (FCR) as compared with the control group (P<0.05). Amylase, protease and lipase activities were noticed markedly higher in fish supplemented with 1.5 × 108 CFU/g LR + 1 g RS/kg and 1.5 × 108 CFU/g LR diets compared to the control (P<0.05). Generally, fish in supplemented diets, particularly T2 and T6 groups, had the highest lysozyme, alternative complement activity (ACH50), total Ig, nitroblue tetrazolium test (NBT), myeloperoxidase (MPO), complement component 3 (C3), complement component 4 (C4), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx). On the other hand, T2 and T6 groups had the lowest malondialdehyde (MDA), glucose, and cortisol concentrations as well as alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) enzyme levels when were compared with the control (P<0.05). After ammonia stress, fish in the supplemented groups, particularly T2 and T6, generally showed significantly higher values of lysozyme, ACH50, total Ig, NBT, MPO, C3, C4, SOD, CAT, GPx and lower levels of MDA, glucose, cortisol, ALT, ALP, LDH when compared with the control (P<0.05). In conclusion, a combined administration of RS and L. rhamnosus effectively improved growth performance and health status as well as enhanced the resistance of rainbow trout against ammonia toxicity.

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Section: Discussionmentioning

confidence: 86%

Rosmarinic acid alone or in combination with Lactobacillus rhamnosus ameliorated resistance to ammonia stress in the rainbow trout, Oncorhynchus mykiss: growth, immunity, antioxidant defense and liver functions

Hajirezaee

Khanjani

2023

Annals of Animal Science

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“…Our study group also demonstrated that commensal Psychrobacter sp. SE6 can regulate intestinal immunity and maintain the balance of intestinal microbiota in grouper (Sun et al 2014), and commensal Lactococcus petauri LF3 and Bacillus siamensis LF4 can significantly increase the activity of serum lysozyme (Yang et al 2021) and regulate the intestinal immunity and microbial structure in Japanese seabass (Lateolabrax japonicus) (Yang et al 2022a(Yang et al , 2022b. It can be concluded that the host-derived beneficial microbes can regulate fish intestinal immune function, control the excessive proliferation of intestinal pathogens, and maintain the intestinal microbial homeostasis.…”

Section: A97mentioning

confidence: 99%

The interactions between the host immunity and intestinal microorganisms in fish

Zhang,

Yang,

Cai

et al. 2024

Appl Microbiol Biotechnol

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There is a huge quantity of microorganisms in the gut of fish, which exert pivotal roles in maintaining host intestinal and general health. The fish immunity can sense and shape the intestinal microbiota and maintain the intestinal homeostasis. In the meantime, the intestinal commensal microbes regulate the fish immunity, control the extravagant proliferation of pathogenic microorganisms, and ensure the intestinal health of the host. This review summarizes developments and progress on the known interactions between host immunity and intestinal microorganisms in fish, focusing on the recent advances in zebrafish (Danio rerio) showing the host immunity senses and shapes intestinal microbiota, and intestinal microorganisms tune host immunity. This review will offer theoretical references for the development, application, and commercialization of intestinal functional microorganisms in fish. Key points • The interactions between the intestinal microorganisms and host immunity in zebrafish• Fish immunity senses and shapes the microbiota • Intestinal microbes tune host immunity in fish

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“…Two host-derived probiotics L. petauri LF3 (GenBank number MZ618882) and B. siamensis LF4 (GenBank number MZ683191) isolated from the gut of Japanese seabass (L. japonicus) were selected and applied for this study. The control diet was formulated with ingredients as described by our previous study (Yang et al, 2021), For probiotic-supplemented diets preparation, two probiotic strains L. petauri LF3 and B. siamensis LF4 were inoculated into nutrient broth liquid, the culture was incubated at 30°C for 24 h. Bacterial cells were centrifuged, washed, and re-suspended in sterile phosphate buffered saline (PBS) before being surface spray to the control diet and air-dried for 24 h to obtain approximately 1 × 10 8 CFU g −1 of diets. All diets were stored at 4°C until use.…”

Section: Probiotic Strains and Experimental Diets Preparationmentioning

confidence: 99%

“…After acclimation for two weeks, fish (average weight 23 g) were divided into three groups with triplicate (three tanks per group) and hand-fed control diet (C), L. petauri LF3 (LF3) and B. siamensis LF4 (LF4) supplemented diets to apparent satiation at 08:30 a.m. and 17:30 p.m. for 6 weeks. Fish husbandry management was in line with Yang et al (2021). At the end of the feeding trial, seabass was anaesthetized with buffered MS-222 (CAS:886-86-2) after 24 h of starvation.…”

Section: Experimental Fish and Samplingmentioning

confidence: 99%

“…Previous study has evaluated the application of five possible host-derived probiotic strains (Bacillus siamensis LF4, Lactococcus petaurid LF3, Micrococcus yunnanensis LD5, Micrococcus aloeverae LC3, Mitsuaria chitosanitabida LD3) in juvenile Japanese seabass (L. japonicus). The results demonstrated that probiotic L. petauri LF3 and B. siamensis LF4 could effectively improve growth performance, innate immunity, antioxidant activity and ammonia tolerance (Yang et al, 2021). To the best of our knowledge, limited information is still available about the impact of host-derived probiotics on intestinal microbiota in Japanese seabass.…”

Section: Introductionmentioning

confidence: 99%

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Host-Derived Probiotics Shape the Intestinal Microbial Composition, but not Putative Function in Juvenile Japanese Seabass (Lateolabrax japonicus)

et al. 2022

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A complex and dynamic microbiota exists in the intestine of fish and plays vital roles in host growth and health. However, the interactions between host originated beneficial bacteria/probiotics with gut microbiota are still largely unknown. The aim of the study is to investigate the impact of two host-derived probiotics, Lactococcus petauri LF3 and Bacillus siamensis LF4, on the intestinal microbiota of juvenile Japanese seabass (Lateolabrax japonicus). Fish were fed the control diet (C), L. petauri LF3 (LF3) and B. siamensis LF4 (LF4) supplemented diets for 6 weeks, and the intestinal microbial composition and function were evaluated by using high-throughput sequencing technology. The relative abundances of certain phyla changed significantly in the probiotic-supplemented groups, Fusobacteria and Proteobacteria decreased, while Firmicutes and Bacteroidetes increased apparently in the groups LF3 and LF4. The apparently increased relative abundances of intestinal possible beneficial Christensenellaceae_R-7_group and Lactobacillus were observed in the groups LF3 and LF4, especially in the group LF4. Compared with the control group (C), Chao1, Ace and Shannon indices enhanced remarkably in groups LF3 and LF4. Intestinal microbiota was determined to have more similarity and lower individual differences based on beta diversity analysis including PCoA, NMDS and UPGMA clustering tree in groups LF3 and LF4 compared with the control group (C). Additionally, Firmicutes as a significant biomarker emerged in the groups LF3 and LF4 compared with the control. The intestinal microbial functions (amino acid metabolism, carbohydrate metabolism, energy metabolism, membrane transport, etc.) did not alter among all groups based on level-2 KEGG pathways. In summary, host- derived probiotic L. petauri LF3 and B. siamensis LF4 shape the intestinal microbial composition, but not function in juvenile Japanese seabass (L. japonicus).

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Host‐associated Bacillus siamensis and Lactococcus petauri improved growth performance, innate immunity, antioxidant activity and ammonia tolerance in juvenile Japanese seabass ( Lateolabrax japonicus )

Cited by 18 publications

References 55 publications

Rosmarinic acid alone or in combination with Lactobacillus rhamnosus ameliorated resistance to ammonia stress in the rainbow trout, Oncorhynchus mykiss: growth, immunity, antioxidant defense and liver functions

Rosmarinic acid alone or in combination with Lactobacillus rhamnosus ameliorated resistance to ammonia stress in the rainbow trout, Oncorhynchus mykiss: growth, immunity, antioxidant defense and liver functions

The interactions between the host immunity and intestinal microorganisms in fish

Host-Derived Probiotics Shape the Intestinal Microbial Composition, but not Putative Function in Juvenile Japanese Seabass (Lateolabrax japonicus)

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