Host Antigens in the Centriolar Region Are Induced in SV40-infected TC7 Cells: SV40 Small-T-function Requirement

Kasamatsu, Harumi; Shyamala, M.; Lin, Woei

doi:10.1101/sqb.1980.044.01.028

Cited by 11 publications

(8 citation statements)

References 32 publications

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“…When cells were infected and transferred at 1.5 h after infection to medium containing actinomycin D, uninfected levels of the antigen persisted for as long as 20 h after infection ( Table 1). We have previously reported similar results, using puromycin as an inhibitor of protein synthesis (16). Therefore, both protein and RNA syntheses are required for stimulation of the centriolar antigen to occur in SV40infected cells.…”

Section: Stimulation Of Host Cell Centriolar Antigen Bysupporting

confidence: 66%

“…The slightly lower antigen levels seen when the inhibitor was added at times after 5 h of infection suggested that some additional stimulation of the antigen would normally be occurring at these later times. We have previously demonstrated a similar effect when infected cells are exposed to the protein synthesis inhibitor puromycin (16).…”

Section: Stimulation Of Host Cell Centriolar Antigen Bymentioning

confidence: 58%

“…It is possible that there is another cellular factor(s) that is responsible for the stimulation of centriolar antigen staining in TC7 cells. We have also shown that stimulation occurs at wild-type levels for temperature-sensitive mutants of the SV40 A, B, C, BC, and D complementation groups (16). Therefore, SV40-specific stimulation of the host centriolar antigen occurred independently of the large-T or structural genes of SV40.…”

Section: Stimulation Of Host Cell Centriolar Antigen Bymentioning

confidence: 69%

“…Although there may be another factor that influences semipermissive host-virus interaction, either cellular or viral, the observation that adenovirus stimulates centriolar antigen in TC7 cells agrees with the result described above and indicates that adenovirus has a gene function that can stimulate the centriolar antigen in TC7 cells. DISCUSSION The infection of TC7 cells by SV40 stimulates certain host cell antigens which are localized in the centriolar region (16). De novo syntheses of both protein (16) and RNA, which occur within the first 5 h after infection, are essential for the stimulation of this antigen by SV40.…”

Section: Downloaded Frommentioning

confidence: 98%

“…We have shown previously that the infection with SV40 of TC7 cells, a subline of AGMK cells, stimulates the appearance of a host cell centriolar antigen which has been localized in the centriolar region of TC7 cells by indirect immunofluorescence microscopy (15). We have also shown that the centriolar antigen consists of two polypeptides of 14,000 and 17,000 daltons (16). The centriolar antigen is stimulated after SV40 infection and is observed before the appearance of T antigen, an early gene product of SV40 (15).…”

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confidence: 89%

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Stimulation of host centriolar antigen in TC7 cells by simian virus 40: requirement for RNA and protein syntheses and an intact simian virus 40 small-t gene function

Shyamala¹,

Atcheson²,

Kasamatsu³

1982

J Virol

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Simian virus 40 (SV40) stimulated a host cell antigen in the centriolar region after infection of African green monkey kidney (AGMK) cells. The addition of puromycin and actinomycin D to cells infected with SV40 within 5 h after infection inhibited the stimulation of the host cell antigen, indicating that de novo protein and RNA syntheses that occurred within the first 5 h after infection were essential for the stimulation. Early viable deletion mutants of SV40 with deletions mapping between 0.54 and 0.59 map units on the SV40 genome, d12000, d12001, d12003, d12004, d12005, dl2006, and d12007, did not stimulate the centriolar antigen above the level of uninfected cells. This indicated that an intact, functional small-t protein was essential for the SV40-mediated stimulation of the host cell antigen. Our studies, using cells infected with nondefective adenovirus-SV40 hybrid viruses that lack the small-t gene region of SV40 (Ad2+ND1, Ad2+ND2, Ad2+ND3, Ad2+ND4, and Ad2+ND5), revealed that the lack of small-t gene function of SV40 could be complemented by a gene function of the adenovirus-SV40 hybrid viruses for the centriolar antigen stimulation. Thus, adenovirus 2 has a gene(s) that is analogous to the small-t gene of SV40 for the stimulation of the host cell antigen in AGMK cells.

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Section: Stimulation Of Host Cell Centriolar Antigen Bysupporting

confidence: 66%

Section: Stimulation Of Host Cell Centriolar Antigen Bymentioning

confidence: 58%

Section: Stimulation Of Host Cell Centriolar Antigen Bymentioning

confidence: 69%

Section: Downloaded Frommentioning

confidence: 98%

mentioning

confidence: 89%

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Stimulation of host centriolar antigen in TC7 cells by simian virus 40: requirement for RNA and protein syntheses and an intact simian virus 40 small-t gene function

Shyamala¹,

Atcheson²,

Kasamatsu³

1982

J Virol

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The centriolar antigen expression in TC7 cells is dependent on growth conditions and occurs at a particular time point in G₁

Segarini¹,

Shyamala²,

Atcheson³

et al. 1983

Journal Cellular Physiology

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The correlation between growth conditions and centriolar antigen (Cag) expression in TC7 cells, a subline of African green monkey kidney cells, was studied. TC7 cells became quiescent when their number reached a high cell density, or when serum factors were depleted from media containing a low concentration of fetal bovine serum (FBS). There was a stoichiometric relationship between the concentration of serum present and the number of new cells produced. During proliferation, the projected cell area decreased as a function of cell density with two abrupt transitions. The first transition appeared to be independent of cell-cell contact. However, the second transition seemed to occur mainly as a result of the limitation of the available substratum surface on which the cells could grow. The appearance of Cag in TC7 cells was found to be associated with the cells' growth conditions as well as with the particular phase in cell cycle. In an exponentially growing culture of cells with 10% FBS and in cells that were growth restricted due to a high density (above 2-3 X 10(5) cells/cm2), the incidence of cells with Cag-positive staining was about 10-20%. It increased, however, to about 40-60% at cell densities between 2 X 10(4) cells/cm2 and 1 X 10(5) cells/cm2. The frequency of cells with positive Cag staining was as high as 80% in TC7 cells that were growth restricted by depletion of serum factor(s). Thus, the quiescent states attained by the two different growth restrictions seem to be different in their ability to express Cag. The frequency of Cag could be further increased by stimulating the quiescent cell population by FBS. In mitotically selected TC7 cells, Cag staining appeared about 4 hr after mitosis and about 2-4 hr before the onset of DNA synthesis. Thus, expression of Cag in TC7 cells is related to their growth conditions, and is characteristic of a part of the G1 phase of the cell cycle.

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Immuno-electron-microscopic localization of a centriole-related antigen in ciliated cells

Fung

Kasamatsu

1985

Cell Tissue Res.

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An antigen common to purported centriolar and basal body regions of a variety of cell types was previously visualized by immuno-fluorescence microscopy. The present study demonstrates the localization of the antigen relative to the defined basal body structures of ciliated tracheal cells at the electron-microscopic level. After ethyldimethylaminopropyl carbodiimide-glutaraldehyde-saponin (EGS) fixation and permeabilization, immunoferritin labeling is consistently found associated with amorphous electron-opaque material in proximity to basal bodies and their ciliary rootlets, but not with basal body microtubules themselves. This distribution pattern is distinct from that of other proteins found in the apical region of ciliated cells, such as calmodulin. It is proposed that the dense material may be analogous to pericentriolar material of centrosomes.

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Host Antigens in the Centriolar Region Are Induced in SV40-infected TC7 Cells: SV40 Small-T-function Requirement

Cited by 11 publications

References 32 publications

Stimulation of host centriolar antigen in TC7 cells by simian virus 40: requirement for RNA and protein syntheses and an intact simian virus 40 small-t gene function

Stimulation of host centriolar antigen in TC7 cells by simian virus 40: requirement for RNA and protein syntheses and an intact simian virus 40 small-t gene function

The centriolar antigen expression in TC7 cells is dependent on growth conditions and occurs at a particular time point in G₁

Immuno-electron-microscopic localization of a centriole-related antigen in ciliated cells

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Host Antigens in the Centriolar Region Are Induced in SV40-infected TC7 Cells: SV40 Small-T-function Requirement

Cited by 11 publications

References 32 publications

Stimulation of host centriolar antigen in TC7 cells by simian virus 40: requirement for RNA and protein syntheses and an intact simian virus 40 small-t gene function

Stimulation of host centriolar antigen in TC7 cells by simian virus 40: requirement for RNA and protein syntheses and an intact simian virus 40 small-t gene function

The centriolar antigen expression in TC7 cells is dependent on growth conditions and occurs at a particular time point in G1

Immuno-electron-microscopic localization of a centriole-related antigen in ciliated cells

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The centriolar antigen expression in TC7 cells is dependent on growth conditions and occurs at a particular time point in G₁