Host and symbiont intraspecific variability: The case of Paramecium calkinsi and “ Candidatus Trichorickettsia mobilis”

Sabaneyeva, Elena; Castelli, Michele; Szokoli, Franziska; Benken, Konstantin A.; Лебедева, Н. В.; Salvetti, Alessandra; Schweikert, Michael; Fokin, Sergei I.; Petroni, Giulio

doi:10.1016/j.ejop.2017.12.002

Cited by 38 publications

(29 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As in Paramecium three different subspecies of RLOs “ Ca . Trichorickettsia mobilis” have been described [63], 16S rRNA gene amplification was performed to identify endosymbionts associated with P. multimicronuclatum US_Bl 16I1 strain (sequence length 1,563 bp, accession number: MK598854), which allowed to assign it to “ Ca . Trichorickettsia mobilis subsp.…”

Section: Resultsmentioning

confidence: 99%

“…Trichorickettsia” was chosen as candidate for trans-infection experiments as it shows a broad ciliate host range, infecting different cell compartment (i.e. cytoplasm and nucleus) of Paramecium multimicronucleatum, Paramecium nephridiatum, Paramecium calkinsi and Euplotes aediculatus [28, 63]. Additionally, in P. multimicronucleatum and P. calkinsi , “ Ca .…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, in P. multimicronucleatum and P. calkinsi , “ Ca . Trichorickettsia” appears covered by long flagella and is able to actively move [28, 63]. Thus, for our research purpose, we selected the newly isolated P. multimicronucleatum strain US_Bl 16I1 as donor host in the trans-infection experiments.…”

Section: Introductionmentioning

confidence: 99%

“…We characterized its macronuclear bacterial symbiont, assigning it to “ Ca . Trichorickettsia mobilis subspecies hyperinfectiva” ( Rickettsiaceae, Rickettsiales ), previously discovered and described in the cytoplasm of Paramecium calkinsi [63]. As recipient, we selected the freshwater planarian Dugesia japonica .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Outwitting planarian’s antibacterial defence mechanisms:Rickettsialesbacterial trans-infection fromParamecium multimicronucleatumto planarians

Modeo

Salvetti

Rossi

et al. 2019

Preprint

View full text Add to dashboard Cite

AS) 22 23 RUNNING PAGE HEAD: Rickettsiales macronuclear endosymbiont of a ciliate can transiently 24 enter planarian tissues 25 Abstract 38 Most of the microorganisms belonging to genera responsible for vector-borne diseases (VBD) have 39 hematophagous arthropods as vector/reservoir. Recently, many new species of microorganisms 40 phylogenetically related to agents of VBD were found in a variety of aquatic eukaryotic hosts, in 41 particular, numerous new bacterial species related to the genus Rickettsia (Alphaproteobacteria,42 Rickettsiales) were discovered in protist ciliates and other unicellular eukaryotes. Although their 43 pathogenicity for humans and terrestrial animals is not known, these bacteria might act as 44 etiological agents of possible VBD of aquatic organisms, with protist as vectors. In the present 45 study, we characterized a novel strain of the Rickettsia-Like Organism (RLO) endosymbiont 46 "Candidatus (Ca.) Trichorickettsia mobilis" in the macronucleus of the ciliate Paramecium 47 multimicronucleatum through Fluorescence In Situ Hybridization (FISH) and molecular analyses.48 Ultrastructural investigations on the presence of flagella confirmed previous studies on the same 49 bacterial species. The potential trans-infection per os of this bacterium to planarians (Dugesia 50 japonica), a widely used model system able to eliminate a wide range of bacteria pathogenic to 51 humans and other Metazoa, was further verified. Ciliate mass cultures were set up, and trans-52 infection experiments were performed by adding homogenized paramecia to food of antibiotic-53 treated planarians, performed. Treated and non-treated (i.e. control) planarians were investigated at 54 day 1, 3, and 7 after feeding for endosymbiont presence by means of PCR and ultrastructural 55 analyses. Obtained results were fully concordant and suggest that this RLO endosymbiont can be 56 transferred from ciliates to metazoans, being detected up to day 7 in treated planarian enterocytes 57 inside and, possibly, outside phagosomes. 58 59 KEY WORDS Planarians, molecular and ultrastructural investigations, Rickettsiaceae, RLO 60 endosymbiont, trans-infection experiments, vector-borne disease 61 4 62 Introduction63 Bacteria of order Rickettsiales (Alphaproteobacteria) live in an obligate association with a wide range 64 of eukaryotes [1-8], and, for their vast majority, are localized intracellularly, although the case of an 65 extracellular Rickettsiales bacterium was recently documented [9]. Rickettsiales are widely studied 66 for their involvement in medical and veterinary fields. Indeed, many of them (e.g. Rickettsia spp., 67 Anaplasma spp., Ehrlichia spp., and Orientia tsutsugamushi) are vectored by lice, ticks and mites, 68 and cause mild to severe disease [1, 10], such as epidemic typhus, Rocky Mountain spotted fever [11-69 13], anaplasmosis, ehrlichiosis [14, 15], heartwater [16], and scrub typhus [17]. 70 In the last two decades, many new genera and species of Rickettsiales were found as symbionts 71 in a variety of other non-vecto...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Outwitting planarian’s antibacterial defence mechanisms:Rickettsialesbacterial trans-infection fromParamecium multimicronucleatumto planarians

Modeo

Salvetti

Rossi

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…The alignment was trimmed at both ends to the length of the shortest sequence, resulting in 1,398 characters (Supplementary material 24). Maximum likelihood (ML) and Bayesian inference phylogenetic analysis were performed as previously described (Sabaneyeva et al 2018), employing respectively PhyML (Guindon and Gascuel 2003) and MrBayes (Ronquist et al 2012), after selection of the best substitution model with jModelTest (Darriba et al 2012). 16S rRNA gene identity values were calculated on the alignment employed for phylogeny.…”

Section: S Rrna Phylogenetic Analysismentioning

confidence: 99%

The extracellular association of the bacterium “CandidatusDeianiraea vastatrix” with the ciliateParameciumsuggests an alternative scenario for the evolution ofRickettsiales

Castelli

Sabaneyeva

Lanzoni

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

Rickettsiales are a lineage of obligatorily intracellular Alphaproteobacteria, encompassing important human pathogens, manipulators of host reproduction, and mutualists. Here we report the discovery of a novel Rickettsiales bacterium associated with Paramecium, displaying a unique extracellular lifestyle, including the ability to replicate outside host cells. Genomic analyses show that the bacterium possesses a higher capability to synthesize amino acids, compared to all investigated Rickettsiales. Considering these observations, phylogenetic and phylogenomic reconstructions, and re-evaluating the different means of interaction of Rickettsiales bacteria with eukaryotic cells, we propose an alternative scenario for the evolution of intracellularity in Rickettsiales. According to our reconstruction, the Rickettsiales ancestor would have been an extracellular and metabolically versatile bacterium, while obligate intracellularity and genome reduction would have evolved later in parallel and independently in different sub-lineages. The proposed new scenario could impact on the open debate on the lifestyle of the last common ancestor of mitochondria within Alphaproteobacteria.

show abstract

The Hidden World of Rickettsiales Symbionts: “Candidatus Spectririckettsia obscura,” a Novel Bacterium Found in Brazilian and Indian Paramecium caudatum

et al. 2018

View full text Add to dashboard Cite

Symbioses between bacteria and eukaryotes are widespread and may have significant impact on the evolutionary history of symbiotic partners. The order Rickettsiales is a lineage of intracellular Alphaproteobacteria characterized by an obligate association with a wide range of eukaryotic hosts, including several unicellular organisms, such as ciliates and amoebas. In this work, we characterized the Rickettsiales symbionts associated with two different genotypes of the freshwater ciliate Paramecium caudatum originated from freshwater environments in distant geographical areas. Phylogenetic analyses based on 16S rRNA gene showed that the two symbionts are closely related to each other (99.4% identity), belong to the family Rickettsiaceae, but are far-related with respect to previously characterized Rickettsiales. Consequently, they were assigned to a new species of a novel genus, namely "Candidatus Spectririckettsia obscura." Screening on a database of short reads from 16S rRNA gene amplicon-based profiling studies confirmed that bacterial sequences related to the new symbiont are preferentially retrieved from freshwater environments, apparently with extremely scarce occurrence (< 0.1% positive samples). The present work provides new information on the still under-explored biodiversity of Rickettsiales, in particular those associated to ciliate host cells.

show abstract

Host and symbiont intraspecific variability: The case of Paramecium calkinsi and “ Candidatus Trichorickettsia mobilis”

Cited by 38 publications

References 73 publications

Outwitting planarian’s antibacterial defence mechanisms:Rickettsialesbacterial trans-infection fromParamecium multimicronucleatumto planarians

Outwitting planarian’s antibacterial defence mechanisms:Rickettsialesbacterial trans-infection fromParamecium multimicronucleatumto planarians

The extracellular association of the bacterium “CandidatusDeianiraea vastatrix” with the ciliateParameciumsuggests an alternative scenario for the evolution ofRickettsiales

The Hidden World of Rickettsiales Symbionts: “Candidatus Spectririckettsia obscura,” a Novel Bacterium Found in Brazilian and Indian Paramecium caudatum

Contact Info

Product

Resources

About