Hospital Acquired Infections by Carbapenem Resistant Enterobacteriacea

Abstract

Background: The emergence and spread of carbapenem resistant Enterobacteriaceae (CRE) infections have become an increasing concern for healthcare services worldwide. Community and hospital-acquired infections caused by these bacteria have been associated with significant morbidity and mortality with limited treatment options. Rapid and accurate detection of carbapenem resistance in these bacteria is important for infection control. Objectives: To detect the prevalence of carbapenem resistant Enterobacteriaceae (CRE) species and determine their antimicrobial susceptibility profile using the Vitek 2 system and detect the presence of carbapenemases genes using Multiplex PCR. Methodology: Various clinical samples were collected from 469 patients admitted in Sohag university hospitals in the period between April 2016 and August 2017, CRE isolates were identified by conventional methods and antimicrobial susceptibility testing using disc diffusion method and also performed by Vitek 2 automated system, Multiplex PCR was used for detection of carbapenemases genes as blaKPC, blaVIM, blaIMP, blaNDM-1 and blaOXA-48. Results: