Horticultural Characteristics of Transgenic Tobacco Expressing the rolC Gene from Agrobacterium rhizogenes

Scorza, R.; Zimmerman, Thomas W.; Cordts, John; Footen, K.J.; Ravelonandro, M.

doi:10.21273/jashs.119.5.1091

Cited by 30 publications

(25 citation statements)

References 21 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consequently, although there was a significantly greater number of roots recovered from the transgenic rootstock, the root dry weight was significantly less than that of the PX1 control rootstock ( Table 1). The characteristics of these 'Paradox' trees transformed with rolABC are consistent with the characteristics of other species transformed with single or multiple rol genes (Rugini et al, 1992;Schmulling et al, 1988;Scorza et al, 1994;Welander et al, 1998). ROOTING LEAFY SEMI-HARDWOOD CUTTINGS.…”

Section: Resultssupporting

confidence: 75%

“…size and larger flowers; rolB increases rooting potential which may result from increased auxin sensitivity of the tissues, alters leaf morphology and increases flower size; rolC reduces internode length, produces flowering abnormalities, and increases branching; and, rolD causes dwarfing and early flowering (Rugini et al, 1997;Schmulling et al, 1988;Scorza et al, 1994).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Rooting and Other Characteristics of a Transgenic Walnut Hybrid (Juglans hindsii × J. regia) Rootstock Expressing rolABC

Vahdati¹,

McKenna²,

Dandekar³

et al. 2002

jashs

View full text Add to dashboard Cite

Walnuts (Juglans spp.) are difficult-to-root woody plants. The rolABC genes (rolA + rolB + rolC), derived from the bacteria Agrobacterium rhizogenes, have been shown to increase the rooting potential of other difficult-to-root woody plants. We inserted the rolABC genes into somatic embryos of a `Paradox' hybrid (J. hindsii × J. regia) clone PX1 using the A. tumefaciens gene transfer system. A transgenic sub-clone, designated PX1 rolABC 2-2 was selected and compared to the untransformed clone for a variety of phenotypic characteristics, including rooting potential. Transformed and untransformed shoots were budded onto seedling J. regia rootstock in the greenhouse and established in the field. Transformed trees displayed reduced internode length, an increase in lateral branching, and wrinkled leaves. In another test, a commercial persian walnut cultivar J. regia `Chandler' was grafted onto rooted cuttings of both the untransformed and transformed plants. The presence of the rolABC genes in the rootstock had no visible effects on the grafted scion. Several of these trees were excavated from the field and the root systems of each genotype were examined for root number, diameter, and biomass. Trees with the rolABC rootstock had significantly more small diameter roots compared to the controls and less recovered biomass. Tests of the rooting potential of leafy semi-hardwood cuttings for two years resulted in 14% to 59% rooting of the transformed cuttings compared to 51% to 81% rooting of the control. Both transformed hardwood cuttings and microshoots in tissue culture also rooted significantly less (52% and 29% respectively) than untransformed hardwood cuttings and tissue cultured shoots (82% and 54% respectively). Thus, although the rolABC genes induced a shorter internode length and a more fibrous root system (typical of rol-tranformed plants), they were not useful for increasing the rooting potential, and as rootstock they did not affect the phenotype of the scion.

show abstract

Section: Resultssupporting

confidence: 75%

mentioning

confidence: 99%

Rooting and Other Characteristics of a Transgenic Walnut Hybrid (Juglans hindsii × J. regia) Rootstock Expressing rolABC

Vahdati¹,

McKenna²,

Dandekar³

et al. 2002

jashs

View full text Add to dashboard Cite

show abstract

“…rhizogenes for co-transformation. Shoot and root phenotypes were consistent with the expected effects of inserted rolABC genes [42-44]. RolA induces wrinkled, slightly curled leaves and dense, bushy foliage in transgenic walnut plants.…”

Section: Discussionsupporting

confidence: 67%

Stacking resistance to crown gall and nematodes in walnut rootstocks

et al. 2013

View full text Add to dashboard Cite

BackgroundCrown gall (CG) (Agrobacterium tumefaciens) and the root lesion nematodes (RLNs) (Pratylenchus vulnus) are major challenges faced by the California walnut industry, reducing productivity and increasing the cost of establishing and maintaining orchards. Current nematode control strategies include nematicides, crop rotation, and tolerant cultivars, but these methods have limits. Developing genetic resistance through novel approaches like RNA interference (RNAi) can address these problems. RNAi-mediated silencing of CG disease in walnut (Juglans regia L.) has been achieved previously. We sought to place both CG and nematode resistance into a single walnut rootstock genotype using co-transformation to stack the resistance genes. A. tumefaciens, carrying self-complimentary iaaM and ipt transgenes, and Agrobacterium rhizogenes, carrying a self-complimentary Pv010 gene from P. vulnus, were used as co-transformation vectors. RolABC genes were introduced by the resident T-DNA in the A. rhizogenes Ri-plasmid used as a vector for plant transformation. Pv010 and Pv194 (transgenic control) genes were also transferred separately using A. tumefaciens. To test for resistance, transformed walnut roots were challenged with P. vulnus and microshoots were challenged with a virulent strain of A. tumefaciens.ResultsCombining the two bacterial strains at a 1:1 rather than 1:3 ratio increased the co-transformation efficiency. Although complete immunity to nematode infection was not observed, transgenic lines yielded up to 79% fewer nematodes per root following in vitro co-culture than untransformed controls. Transgenic line 33-3-1 exhibited complete crown gall control and 32% fewer nematodes. The transgenic plants had thicker, longer roots than untransformed controls possibly due to insertion of rolABC genes. When the Pv010 gene was present in roots with or without rolABC genes there was partial or complete control of RLNs. Transformation using only one vector showed 100% control in some lines.ConclusionsCG and nematode resistance gene stacking controlled CG and RLNs simultaneously in walnuts. Silencing genes encoding iaaM, ipt, and Pv010 decrease CG formation and RLNs populations in walnut. Beneficial plant genotype and phenotype changes are caused by co-transformation using A. tumefaciens and A. rhizogenes strains. Viable resistance against root lesion nematodes in walnut plants may be accomplished in the future using this gene stacking technology.

show abstract

“…The tobacco plants were transformed to serve as positive controls for GUS and molecular assays of transformation with the vector used in this study. The tobacco transformation protocol was as described by Scorza et al (1994a). Specific oligonucleotide primers at the 5' and 3' end of the PRVCP, GUS and NPTII genes were used to identify the presence of these genes in DNA isolated from different transgenic clones.…”

Section: Confirmation Of Transformationmentioning

confidence: 99%

Transformation of Plum with the Papaya Ringspot Virus Coat Protein Gene and Reaction of Transgenic Plants to Plum Pox Virus

Scorza¹,

Lévy²,

Damsteegt³

et al. 1995

jashs

View full text Add to dashboard Cite

Transgenic plum plants expressing the papaya ringspot virus (PRV) coat protein (CP) were produced by Agrobacterium-mediated transformation of hypocotyl slices. Hypocotyl slices were cocultivated with Agrobacterium tumefaciens strain C58/Z707 containing the plasmid pGA482GG/CPPRV-4. This plasmid carries the PRVCP gene construct and chimeric NPTII and GUS genes. Shoots were regenerated on Murashige and Skoog salts, vitamins, 2% sucrose, 2.5 μm indolebutyric acid, 7.5 μm thidiazuron, and appropriate antibiotics for selection. Integration of the foreign genes was verified through kanamycin resistance, GUS assays, polymerase chain reaction (PCR), and Southern blot analyses. Four transgenic clones were identified. Three were vegetatively propagated and graft-inoculated with plum pox virus (PPV)-infected budwood in a quarantine, containment greenhouse. PPV infection was evaluated over a 2- to 4-year period through visual symptoms, enzyme-linked immunosorbent assay, and reverse transcriptase PCR assays. While most plants showed signs of infection and systemic spread of PPV within l-6 months, one plant appeared to delay the spread of virus and the appearance of disease symptoms. Virus spread was limited to basal portions of this plant up to 19 months postinoculation, but, after 32 months symptoms were evident and virus was detected throughout the plant. Our results suggest that heterologous protection with PRVCP, while having the potential to delay PPV symptoms and spread throughout plum plants, may not provide an adequate level of long-term resistance.

show abstract

Horticultural Characteristics of Transgenic Tobacco Expressing the rolC Gene from Agrobacterium rhizogenes

Cited by 30 publications

References 21 publications

Rooting and Other Characteristics of a Transgenic Walnut Hybrid (Juglans hindsii × J. regia) Rootstock Expressing rolABC

Rooting and Other Characteristics of a Transgenic Walnut Hybrid (Juglans hindsii × J. regia) Rootstock Expressing rolABC

Stacking resistance to crown gall and nematodes in walnut rootstocks

Transformation of Plum with the Papaya Ringspot Virus Coat Protein Gene and Reaction of Transgenic Plants to Plum Pox Virus

Contact Info

Product

Resources

About