Horseradish peroxidase study in rat of the neural connections of the organum vasculosum of the lamina terminalis

Camacho, Arturo; Phillips, M. Ian

doi:10.1016/0304-3940(81)90391-8

Cited by 121 publications

(51 citation statements)

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“…It has been shown that the MePO projects heavily to neurosecretory neurons in the magnocellular part of the PVH and the supraoptic nucleus Weiss and Hatton, 1990) and into the circumventricular organs (Camacho and Phillips, 1981;Lind et al, 1982;Oldfield et al, 1992). However, in the present study, only a few Ucn III fibers were observed in these areas, although Ucn III-positive cells were found in the MePO.…”

Section: Ucn III Fiber Distributioncontrasting

confidence: 54%

Urocortin III-Immunoreactive Projections in Rat Brain: Partial Overlap with Sites of Type 2 Corticotrophin-Releasing Factor Receptor Expression

et al. 2002

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Urocortin (Ucn) III, or stresscopin, is a new member of the corticotropin-releasing factor (CRF) peptide family identified in mouse and human. Pharmacological studies showed that Ucn III is a high-affinity ligand for the type 2 CRF receptor (CRF-R2). To further understand physiological functions the peptide may serve in the brain, the distribution of Ucn III neurons and fibers was examined by in situ hybridization and immunohistochemistry in the rat brain. Ucn III-positive neurons were found predominately within the hypothalamus and medial amygdala. In the hypothalamus, Ucn III neurons were observed in the median preoptic nucleus and in the rostral perifornical area lateral to the paraventricular nucleus. The Ucn III fibers were distributed mainly in the hypothalamus and limbic structures. Hypothalamic regions that were innervated prominently by Ucn III fibers included the ventromedial nucleus, medial preoptic nucleus, and ventral premammillary nucleus. Outside the hypothalamus, the densest projections were found in the intermediate part of the lateral septum, posterior division of the bed nucleus stria terminalis, and the medial nucleus of the amygdala. Several major Ucn III terminal fields identified in the present study, including the lateral septum and the ventromedial hypothalamus, are known to express high levels of CRF-R2. Thus, these anatomical data strongly support the notion that Ucn III is an endogenous ligand for CRF-R2 in these areas. These results also suggest that Ucn III is positioned to play a role in mediating physiological functions, including food intake and neuroendocrine regulation.

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Section: Ucn III Fiber Distributioncontrasting

confidence: 54%

Urocortin III-Immunoreactive Projections in Rat Brain: Partial Overlap with Sites of Type 2 Corticotrophin-Releasing Factor Receptor Expression

et al. 2002

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“…itself, a plausible hypothesis since it is known that at least two types of neuronal elements exist within the body of the o.v.l.t. (Brizzee, 1955;Weindl et al 1968; Moore, 1977;Camacho & Philips, 1981). In that case the action of the lesion within the o.v.l.t.…”

Section: Discussionmentioning

confidence: 97%

Evidence for the involvement of the organum vasculosum laminae terminalis in the febrile response of rabbits and rats.

Stitt¹

1985

The Journal of Physiology

142

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SUMMARY1. Control febrile responses to intravenous injections of endogenous pyrogen were determined in groups of rabbits and rats, exposed to their respective thermoneutral ambient temperatures.2. Discrete electrolytic lesions were placed within the confines of the organum vasculosum laminae terminalis (o.v.l.t.) of each species. The fever responses of the animals were remeasured 3 days later, and in every case there was a marked enhancement of the fevers produced, using the same doses of endogenous pyrogen that were used earlier.3. Similar lesions that were placed unilaterally within the preoptic anterior hypothalamic area in groups of control animals, were not effective in enhancing the febrile responses of these animals.4. The course of this lesion-induced fever enhancement in rabbits was studied during the succeeding 3 week period, when it was found to return gradually towards the control levels.5. It is postulated that the o.v.l.t. is involved in some manner in the process whereby circulating pyrogen is translated into a febrile stimulus. Lesions posited within this region are thought to either increase the amount of pyrogen entering the o.v.l.t., or to increase the sensitivity of pyrogen receptor sites within the o.v.l.t.

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“…The lamina terminalis is now well established as a primary site for osmoreception in the brain (12,36,37,55,69,70). Furthermore, based on injections of tracers in the OVLT but not restricted to it, there appear to be efferent connections from the region to the dorsal midline of the thalamus (4,10,14,15,23,61). The present study provides functional evidence, in the form of Fos labeling following osmotic challenges, that the OVLT relays osmotic signals directly to the dorsal midline of the thalamus, which contains the paraventricular and medial part of the dorsomedial thalamic nucleus.…”

Section: Discussionmentioning

confidence: 99%

The trajectory of sensory pathways from the lamina terminalis to the insular and cingulate cortex: a neuroanatomical framework for the generation of thirst

Hollis¹,

McKinley²,

D'Souza³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

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The trajectory of sensory pathways from the lamina terminalis to the insular and cingulate cortex: a neuroanatomical framework for the generation of thirst. Am J Physiol Regul Integr Comp Physiol 294: R1390-R1401, 2008. First published January 30, 2008 doi:10.1152/ajpregu.00869.2007The pathways involved in the emotional aspects of thirst, the arousal and affect associated with the generation of thirst and the motivation to obtain satiation, have been studied but remain poorly understood. Rats were therefore injected with the neurotropic virus pseudorabies in either the insular or cingulate cortex. After 2 days of infection, pseudorabies-positive neurons were identified within the thalamus and lamina terminalis. In a separate group of rats, the retrograde tracer cholera toxin subunit b (CTb) was used in combination with either isotonic (0.15 M NaCl) or hypertonic (0.8 M NaCl) saline (1 ml/100 g body wt ip). Rats injected with CTb in the insular cortex and stimulated with hypertonic saline had increased numbers of Fos/CTb double-positive neurons in the paraventricular, rhomboid, and reuniens thalamic nuclei, whereas those rats injected with CTb in the cingulate cortex and challenged with hypertonic saline had increased numbers of Fos/CTb double-positive neurons in the medial part of the mediodorsal, interanteromedial, anteromedial, and ventrolateral part of the laterodorsal thalamic nuclei. Rats injected with CTb in the dorsal midline of the thalamus and challenged with hypertonic saline had increased numbers of Fos/CTb double-positive neurons within the organum vasculosum of the lamina terminalis (OVLT), median preoptic nucleus, and insular cortex but not the subfornical organ. A small proportion of the CTb-positive neurons in the OVLT were immunopositive for transient receptor potential vanilloid 1, a putative osmoresponsive membrane protein. These results identify functional thalamocortical pathways involved in relaying osmotic signals to the insular and cingulate cortex and may provide a neuroanatomical framework for the emotional aspects of thirst. organum vasculosum; subfornical organ; hypertonic saline; pseudorabies; cholera toxin THE LAMINA TERMINALIS, which contains the organum vasculosum of the lamina terminalis (OVLT), subfornical organ, and median preoptic nucleus, relays osmotic and endocrine signals to the paraventricular (PVN) and supraoptic (SON) nuclei of the hypothalamus to regulate vasopressin secretion (36,38,44,45,55,64,69,70). Putative osmoresponsive membrane proteins expressed within the lamina terminalis, transient receptor potential vanilloid (TRPV) 1 and TRPV4, are important for osmoreception and subsequent vasopressin secretion following hyperosmotic stimulation (12,30,31,59). The lamina terminalis contains efferent projections to regions other than the PVN and SON (34,39,46), including the lateral hypothalamus (4), periaqueductal gray (33), and thalamus (10, 50), suggesting that the lamina terminalis may be important for physiological responses to osmotic signals other than neuroendocr...

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Horseradish peroxidase study in rat of the neural connections of the organum vasculosum of the lamina terminalis

Cited by 121 publications

References 5 publications

Urocortin III-Immunoreactive Projections in Rat Brain: Partial Overlap with Sites of Type 2 Corticotrophin-Releasing Factor Receptor Expression

Urocortin III-Immunoreactive Projections in Rat Brain: Partial Overlap with Sites of Type 2 Corticotrophin-Releasing Factor Receptor Expression

Evidence for the involvement of the organum vasculosum laminae terminalis in the febrile response of rabbits and rats.

The trajectory of sensory pathways from the lamina terminalis to the insular and cingulate cortex: a neuroanatomical framework for the generation of thirst

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