Horseradish peroxidase-catalyzed hydrogelation of fish gelatin with tunable mechanical properties and biocompatibility

Abstract

Horseradish peroxidase-catalyzed injectable gelatin hydrogels have attracted much attention in various biomedical fields because of their processability, biodegradability, and excellent biocompatibility in promoting cell adhesion and proliferation. However, gelatin derivatives are mainly obtained from mammalian sources (porcine, bovine) with thermal gelation at room temperature, leading to the potential problems in biofabrication applications. Here, we introduce a novel fish gelatin derivative that can be easily dissolved and cross-linked at room temperature by horseradish peroxidase. This system provides thermally stable fish gelatin hydrogels with tunable mechanical and biological properties, comparable to porcine gelatin hydrogels. The properties (gelation time, stiffness, degradation rate) of hydrogels prepared from fish gelatin-hydroxyphenyl propionic acid (FGH) are controllable for suitable applications. Moreover, FGH hydrogels allow human dermal fibroblast cells to adhere, proliferate, and produce the extracellular components. These results suggest horseradish peroxidase-cross-linked FGH as potential hydrogel matrices that can be used as an alternative for mammalian gelatin hydrogels in various biomedical applications.

Preparation of Multifunctional Hydrogels with In Situ Dual Network Structure and Promotion of Wound Healing

“…Following 12 h of stirring, the synthesized polymer solution was filtered by dialysis with deionized water using a dialysis bag ( M w : 3500), and dialysis was completed when the absorbance peak at 275 nm (the specific peak of Tyr) was not detectable in the filtered solution. The sample was subsequently lyophilized to obtain Tsg-Tyr …”

“…The sample was subsequently lyophilized to obtain Tsg-Tyr. 32 The content of the introduced tyrosyl groups was tested by an ultraviolet−visible (UV−vis; Evolution 220, Thermo Fisher) spectrophotometer. FucC-Tyr and Tsg-Tyr were mixed in distilled water at 0.1% (w/v), respectively, and absorbance was measured at 275 nm.…”

Supramolecular Gels Incorporating Cordyline terminalis Leaf Extract as a Polyphenol Release Scaffold for Biomedical Applications

“…However, these chemical reactions utilized catalysts, initiators, or oxidizing agents that might interact with drugs/cells encapsulated inside hydrogels. Among them, Horseradish peroxidase-catalyzed hydrogels were formed in the mildest conditions and showed tunable mechanical properties and biocompatibility [6]. These hydrogels were applied successfully to incorporate various cell lines and sustainably release many types of drugs [3,7,8].…”

Unique Enzyme Activity of Peroxidase on a Clay Nanosheet

“…Furthermore, because SSA has a high transparency at the dispersion state in water, it enables the spectroscopic measurement of the adsorbed substance. , The changes in the protein structure and enzyme activity can be evaluated from spectroscopic measurements when proteins were adsorbed on SSA. HRP is one of the heme-containing enzymes that has been actively studied, and its enzyme activity to decompose peroxides is widely used in industrial and medical fields such as in bioassays. − The adsorption of HRP on inorganic material surfaces such as montmorillonite, silica, α-ZrP, tinania nanosheet, and layered double hydroxide has been reported. The decrease of enzyme activity and the improvement of stability − against environmental changes were reported.…”