Horseradish peroxidase‐based hybrid nanoflowers with enhanced catalytical activities for polymerization reactions of phenol derivatives

Abstract

Catalytic activity and stability of HRP‐Cu2+ hybrid nanoflowers (hCu‐NFs) in the polymerization reactions of phenol derivatives was investigated. It was observed that the catalytic activity and stability of hybrid nanoflowers on the polymerization of the phenol derivatives was considerably higher compared to free Horseradish peroxidase (HRP) enzyme. The hCu‐NFs effectively polymerized phenolic compounds as a novel nanobiocatalyst and led to polymers having quite high yields, molecular weights, and thermal stabilities compared to free HRP enzyme. The hCu‐NFs provide substantial repeated use and showed some degree of catalytic activity even after fourth cycle experiment in the polymerization reactions.

Electropolymerizations of two novelEDOT‐BODIPYzincoxide nanocomposites andevaluation of their in vitro antibacterial activities

“…This voltammogram and increase between sequenced cycles were completely different from those of both M2, P(M1‐co‐Edot)/ZnO and pure PEDOT (Figure 10B). As known in the literature, the current increases when polymerization occurs on the electrode surface 3,4 . Similarly, new and different reduction‐oxidation peaks were formed due to the addition of ZnO nanoparticles (Figure 10B).…”

“…Without isolation, the dipyrone intermediate was then reacted with Et 3 N and BF 3. Et 2 O to yield green solid 5,5‐difluoro‐10‐(4‐hydroxyphenyl)‐5H‐dipyrrolo [1,2‐c:2′,1′‐f] 1‐3 diazaborinin‐4‐ium‐5‐uide (M2) BODIPY dye.…”

“…Diminishing nonrenewable energy sources and increasing growth of energy consumption cause demand towards renewable energy sources 1,2 . These requirements have led to intense research on organic conducting polymers with great potentials for practical applications, such as organic solar cells (OSCs), 4 nonlinear optics and electrochromic devices (ECDs), batteries, electronic devices, sensors and capacitors, 3,5 and electromagnetic radiation shielding 5,6 . Similarly, the hybridization of inorganic nanoparticles with conducting polymers is good candidates for many applications, such as biodegradable materials depending on their optoelectronic properties, organic optoelectronic devices and solar cells 4 .…”

Flowerlike hybrid horseradish peroxidase nanobiocatalyst for the polymerization of guaiacol

“…M n : the number average molecular weight, Ð: heterogeneity index. deactivated at temperatures around 60 °C due to the denaturation of the enzyme [32][33][34]. However, the highest yielded polymerization of guaiacol with HRP-Cu 2+ was achieved at 60 °C.…”

“…The optimum polymerization of guaiacol with 51% yield and 9600 g/mol molecular weight was accomplished with 5 weight% of HRP enzyme in pH 7.4 PBS buffer at 30 °C (Table 2; entry 13). Increasing the reaction temperature to 40 °C (Table 2; entry 14) resulted in a decrease of the polymerization yield (35%) since HRP is sensitive to heating and it denatures around 60 °C [32][33][34]. Enhancing HRP concentration to 10 weight% (Table 2; entry 15) slightly increased the yield (53%) and molecular weight (10,800 g/mol) of the product.…”