Hormones in the nucleus. Immunologically demonstrable biogenic amines (serotonin, histamine) in the nucleus of rat peritoneal mast cells

Csaba, G.; Kovács, P.; Pállinger, Éva

doi:10.1016/j.lfs.2005.08.044

Cited by 20 publications

(21 citation statements)

References 31 publications

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“…As a result, not only can cytoplasmic amines be demonstrated more clearly, but nuclear serotonin and histamine contents were also demonstrated [16]. In the present case, nuclear serotonin fluorescence was seen clearly (Fig.…”

Section: Discussionsupporting

confidence: 66%

“…The role of serotonin localized in the nucleus is presently unknown as is also the case with histamine [16]. In addition, there are no characteristic changes in the phenotype of the HDCKO animals, which can be ascribed to the nuclear absence of these molecules.…”

Section: Discussionmentioning

confidence: 98%

“…In these mice the cells do not contain histamine if the animals are kept on histamine-free food. However, in mast cell granules as well as in the nucleus, histamine is present together with another biogenic amine, serotonin [16], the concentration of which could be changed in the absence of histamine. The aim of the present experiment was to study the serotonin content of immune cells in histamine deficient, HDCKO mice.…”

Section: Introductionmentioning

confidence: 99%

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Serotonin content is elevated in the immune cells of histidine decarboxylase gene knock-out (HDCKO) mice. Focus on mast cells

et al. 2007

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Serotonin content is elevated in the immune cells of histidine decarboxylase gene knock-out (HDCKO) mice. Focus on mast cells

et al. 2007

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“…Fluoxetine is a serotonin reuptake inhibitor in the central nervous system as well as in mast cells. It depleted mast cell nuclear as well as cytoplasmic serotonin content [23] . Several researches found that antidepressants influenced histamine and serotonin secretion from rat peritoneal mast cells [24,25] .…”

Section: Issnmentioning

confidence: 98%

Effects of fluoxetine on mast cell morphology and protease-1 expression in gastric antrum in a rat model of depression

Chen¹,

Xiao²,

Chen³

et al. 2008

WJG

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The average level of rMCP-1mRNA of gastric antrum significantly increased in depressed model group (0.759 ± 0.357 vs 0.476 ± 0.029, P < 0.01) or saline + depressed model group (0.781 ± 0.451 vs 0.476 ± 0.029, P < 0.01 ), while no significant difference was found between fluoxetine + normal control group (0.460 ± 0.027) or fluoxetine + depressed model group (0.488 ± 0.030) and normal control group. Fluoxetine showed partial inhibitive effects on mast cell ultrastructural alterations and de-regulated rMCP-1 expression in gastric antrum of the depressed rat model. CONCLUSION: Chronic stress can induce mast cell proliferation, activation, and granule hyperplasia in gastric antrum. Fluoxetine counteracts such changes in the depressed rat model. INTRODUCTIONMast cells are now recognized as "granular cells of the connective tissue", whose activation exacerbates allergic immune responses and as key players in the establishment of innate immunity as well as modulators of adaptive immune responses [1] . The role of mast cells in the gastrointestinal mucosa is not only to react to antigens, but also to actively regulate the barrier and transport properties of the intestinal epithelium. Mucosal mast cells respond to both IgE/antigen-dependent and nonIgE-dependent stimulation, releasing bioactive mediators into adjacent tissues where they induce physiological responses. Studies in models of hypersensitivity and stress Abstract AIM: To investigate the effects of fluoxetine on depression-induced changes of mast cell morphology and protease-1 (rMCP-1) expression in rats. METHODS: A Sprague-Dawley rat model of chronic stress-induced depression was established. Fifty experimental rats were randomly divided into the following groups: normal control group, fluoxetine + normal control group, depressed model group, saline + depressed model group, and fluoxetine + depressed model group. Laser scanning confocal microscopy (LSCM) immunofluorescence and RT-PCR techniques were used to investigate rMCP-1 expression in gastric antrum. Mast cell morphology was observed under transmission electron microscopy. ANOVA was used for statistical analysis among groups. RESULTS: Morphologic observation indicated that depression induced mast cell proliferation, activation, and granule hyperplasia. Compared with the normal control group, the average immunofluorescence intensity of gastric antrum rMCP-1 significantly increased in depressed model group (37.4 ± 7.7 vs 24.5 ± 5.6, P < 0.01) or saline + depressed model group (39.9 ± 5.0 vs 24.5 ± 5.6, P < 0.01), while there was no significant difference between fluoxetine + normal control group (23.1 ± 3.4) or fluoxetine + depressed model group (26.1 ± 3.6) and normal control group.

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“…9 Serotonin is present not only in the cytoplasm of mast cells, but also in the nucleus. 10 A number of drugs influence the serotonin content of nerve cells. Parachlorophenylalanine (PCPA) inhibits tryptophan hydroxylase, decreasing the serotonin level; 11 fluoxetine inhibits 5-HT reuptake 12 and clorgyline blocks monoamino-oxydase (MAO-A), 13 an enzyme which decreases the serotonin level.…”

Section: Introductionmentioning

confidence: 99%

Effect of serotonin‐acting agents on the serotonin content of immune cells. A peculiar observation

Pállinger

Csaba

2006

Cell Biochemistry & Function

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The effect of the tryptophan hydroxylase inhibitor, PCPA methylester, the serotonin reuptake inhibitor fluoxetine and MAO-A inhibitor clorgyline on the serotonin content of rat immune cells was studied, using labelled antibodies and flow cytometry. Each molecule significantly increased in males the serotonin concentration of peritoneal lymphocytes and the monocyte-macrophage-granulocyte group (mo-gran), however the agents were ineffective towards mast cells. In females fluoxetine and clorgyline increased the serotonin concentration in peritoneal lymphocytes and mo-gran. Fluoxetine also increased the serotonin level in mast cells. Thymus was absolutely resistant to the drugs in both genders. The results call attention (1) to the reverse effect of serotonin-acting agents on immune cells, (2) to the influence of the milieu where the cell is located and (3) the effect of gender.

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Hormones in the nucleus. Immunologically demonstrable biogenic amines (serotonin, histamine) in the nucleus of rat peritoneal mast cells

Cited by 20 publications

References 31 publications

Serotonin content is elevated in the immune cells of histidine decarboxylase gene knock-out (HDCKO) mice. Focus on mast cells

Serotonin content is elevated in the immune cells of histidine decarboxylase gene knock-out (HDCKO) mice. Focus on mast cells

Effects of fluoxetine on mast cell morphology and protease-1 expression in gastric antrum in a rat model of depression

Effect of serotonin‐acting agents on the serotonin content of immune cells. A peculiar observation

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