Hormone-regulated expansins: Expression, localization, and cell wall biomechanics in Arabidopsis root growth

Samalova, Marketa; Melnikava, Alesia; Elsayad, Kareem; Peaucelle, Alexis; Gahurova, Evelina; Gumulec, Jaromír; Zemlyanskaya, Elena V.; Ubogoeva, Elena V.; Balkova, Darina; Demko, Martin; Blavet, Nicolas; Alexiou, Panagiotis; Beneš, Vladimír; Mouille, Grégory; Hejátko, Jan

doi:10.1093/plphys/kiad228

Cited by 16 publications

(14 citation statements)

References 105 publications

(177 reference statements)

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“…Chickpea seeds, for instance, express auxin signaling genes in preparation for germination (Kudapa et al., 2018). In the embryonic axes of developing seeds, auxin signaling has been shown to loosen cell walls by activating expansins and xyloglucan endotransglycosylases/hydrolases (XTHs), thereby enabling the cells to expand without automatically losing their integrity (Gimeno‐Gilles et al., 2009; Samalova et al., 2023; Wang & Ruan, 2013). As a result of cell wall expansion, the cell wall can become more vulnerable to the activity of cell wall modification enzymes like pectin methylesterases.…”

Section: Discussionmentioning

confidence: 99%

“…As a result of cell wall expansion, the cell wall can become more vulnerable to the activity of cell wall modification enzymes like pectin methylesterases. One study showed that the overexpression of expansin enzymes was able to increase the rate of pectin demethylesterification in Arabidopsis root cells within 3 h, leading to stiffer cell walls within 6 h (Samalova et al., 2023). Changes in cell wall integrity could then lead to changes in cooking time (Chen et al., 2022; Chigwedere et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

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Gene expression profiling of soaked dry beans (Phaseolus vulgaris L.) reveals cell wall modification plays a role in cooking time

Jeffery,

Mudukuti,

Buell

et al. 2023

The Plant Genome

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Dry beans (Phaseolus vulgaris L.) are a nutritious food, but their lengthy cooking requirements are barriers to consumption. Presoaking is one strategy to reduce cooking time. Soaking allows hydration to occur prior to cooking, and enzymatic changes to pectic polysaccharides also occur during soaking that shorten the cooking time of beans. Little is known about how gene expression during soaking influences cooking times. The objectives of this study were to (1) identify gene expression patterns that are altered by soaking and (2) compare gene expression in fast‐cooking and slow‐cooking bean genotypes. RNA was extracted from four bean genotypes at five soaking time points (0, 3, 6, 12, and 18 h) and expression abundances were detected using Quant‐seq. Differential gene expression analysis and weighted gene coexpression network analysis were used to identify candidate genes within quantitative trait loci for water uptake and cooking time. Genes related to cell wall growth and development as well as hypoxic stress were differentially expressed between the fast‐ and slow‐cooking beans due to soaking. Candidate genes identified in the slow‐cooking beans included enzymes that increase intracellular calcium concentrations and cell wall modification enzymes. The expression of cell wall‐strengthening enzymes in the slow‐cooking beans may increase their cooking time and ability to resist osmotic stress by preventing cell separation and water uptake in the cotyledon.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of soaked dry beans (Phaseolus vulgaris L.) reveals cell wall modification plays a role in cooking time

Jeffery,

Mudukuti,

Buell

et al. 2023

The Plant Genome

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“…Remarkably, at higher N concentrations, H. seropedicae promoted other benefits to the plant, such as shoot length growth, without gain of dry mass ( Figure 9 ). EXPA1 was also up-regulated in INN+ vs CTN+, suggesting changes in the chemical composition of the cell wall that could be possibly modulating root development in response to inoculation under N+, as observed in the overexpression of EXPA1 in Arabidopsis mutant seedlings ( Samalova et al., 2023 ). Thus, our molecular and phenotypic results suggested that under conditions of high N availability, and in the presence of H. seropedicae , there is growth promotion in maize plants, without gain of dry mass.…”

Section: Discussionmentioning

confidence: 89%

“…Expansin proteins are known for their multifaceted roles in plant growth, especially cell walls. Overexpression of EXPA1 was accompanied by changes in the chemical composition of the cell wall leading to changes in root development in Arabidopsis seedlings ( Samalova et al., 2023 ), suggesting that EXPA , together with several genes encoding enzymes involved in cell wall remodeling, may be part of the regulatory network involved in elongation/cell expansion, regulated by GA.…”

Section: Resultsmentioning

confidence: 99%

Dual RNA-seq of maize and H. seropedicae ZAE94 association, in different doses of nitrate, reveals novel insights into Plant-PGPB-environment relationship

Rosman,

Urquiaga,

Thiebaut

et al. 2024

Front. Plant Sci.

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The interactions between plants, beneficial bacteria and their environment are profoundly shaped by various environmental factors, including light, temperature, water availability, and soil quality. Despite efforts to elucidate the molecular mechanisms involved in the association between plants and beneficial bacteria, like Plant Growth-Promoting Bacteria (PGPB), with many studies focusing on the transcriptional reprogramming in the plant, there is no report on the modulation of genetic controls from both plant and associated bacteria standpoints, in response to environment. The main goal of this study was to investigate the relationship between plant-bacteria-environment signaling, using as a model maize plants inoculated with H. seropedicae ZAE94 and cultivated with different doses of N (0.3 and 3 mM). For this purpose, we performed rRNA-depleted RNA-seq to determine the global gene expression of both maize roots and associated H. seropedicae ZAE94. Our results revealed a differential modulation of maize nitrogen metabolism, phytohormone and cell wall responses when associated with H. seropedicae ZAE94 at different N concentrations. In parallel, a modulation of the bacterial metabolism could be observed, by regulating genes involved in transport, secretion system, cell mobility, oxidoreductases, and chemotaxis, when bacteria were associated with maize roots and cultivated at different doses of N. The molecular and phenotypic data of maize plantlets suggested that different doses of N fertilization differentially regulated the beneficial effects of bacterial inoculation, as higher doses (3 mM) favored shoot elongation and lower doses (0.3 mM) favored increase in plant biomass. Our results provide a valuable integrated overview of differentially expressed genes in both maize and associated H. seropedicae ZAE94 in response to different N availability, revealing new insights into pathways involved in grass-PGPB associations.

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“…The specificity of expression and localisation of several alpha-expansins was studied in Arabidopsis thaliana (Samalova et al 2023).…”

Section: Expansins Induce Cell Wall Remodellingmentioning

confidence: 99%

Responses to abiotic and biotic stresses - from the cellular level to fruit development - contributions of the Czech Centre for Experimental Plant Biology

Vaňková¹,

Burketová²,

Brzobohatý³

et al. 2023

Biol. plant.

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The "Centre for Experimental Plant Biology", a joint project of the Institute of Experimental Botany of the Czech Academy of Sciences and CEITEC (represented by Mendel and Masaryk Universities), focused on elucidating the mechanisms of plant responses to abiotic and biotic stresses and their combinations at the cellular level, in intact plants during vegetative and reproductive stages, and fruit development. The consortium demonstrated the importance of shared research facilities, complementary approaches, and knowledge exchange, addressing demanding questions in plant biology. The consortium made breakthrough in plant-pathogen interactions, including identification of exocyst-syntaxin cooperation in non-host resistance. The results confirmed the fundamental role of phytohormones in stress responses, including negative correlation of leaf bioactive gibberellins with drought stress, and the role of cytokinins in ROS homeostasis, sulphur metabolism, and heat stress responses, including volatile emission. Molecular analyses revealed expansin-mediated cell wall remodelling, brassinosteroid-mediated regulation of root growth through PIN2, the role of ALBA and LARP6C proteins in pollen development under abiotic stress, and heat stress impact on fertilization rate, embryo and seed development. Gene Set Enrichment and RNA-Seq analyses allowed to identify crucial genes involved in the apple scab resistance network. The main results obtained during the five-year project are summarised here.

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Hormone-regulated expansins: Expression, localization, and cell wall biomechanics in Arabidopsis root growth

Cited by 16 publications

References 105 publications

Gene expression profiling of soaked dry beans (Phaseolus vulgaris L.) reveals cell wall modification plays a role in cooking time

Gene expression profiling of soaked dry beans (Phaseolus vulgaris L.) reveals cell wall modification plays a role in cooking time

Dual RNA-seq of maize and H. seropedicae ZAE94 association, in different doses of nitrate, reveals novel insights into Plant-PGPB-environment relationship

Responses to abiotic and biotic stresses - from the cellular level to fruit development - contributions of the Czech Centre for Experimental Plant Biology

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