Hormone-Producing Cultures of Adrenal and Pituitary Tumor Origin

Abstract

ions on either side of the membrane, although the action potential is prolonged. Changes in the concentration of H, Ca, or Mg ions may block conduction. The resting and action potentials are somewhat sensitive to Na-ion concentration. These effects are consistent with a model that emphasizes the multilayer structure of the nerve membrane and the divalent ions therein. We wish to express our deep gratitude to Prof. Katsuki of Tokyo Medical and Dental University and to Drs. Hiramoto, Kanatani and Nomura of Tokyo University Marine Station for their constant help during the present experiment.

Expression of Liver Phenotypes in Cultured Mouse Hepatoma Cells<xref ref-type="fn" rid="FN2">2</xref>

“…The cells were passaged from animal to in vitro culture six times over a period of 6 months. This procedure for cell selection was described by Buonasissi et al (6). After passage 6, the cells, designated Hepa, grew in culture with a generation time of about 30 hours.…”

A tribute to Dr. Gordon Hisashi Sato (December 24, 1927–March 31, 2017)

“…Armed with this insight, the Sato lab devised a protocol for selecting differentiated tumor cells that were able to survive and proliferate in culture; this protocol consisted of multiple cycles of alternately passaging tumor cells in vivo and in vitro. Using this approach, they isolated continuous mammalian cell lines from rodent endocrine tumors provided by Jacob Furth (Buonassisi et al 1962;Stollar et al 1964;Yasumura et al 1966;Tashjian et al 1968;Cuprak and Sato 1968) and cell lines from other differentiated tissues (Mohit and Sato 1967;Benda et al 1968;Augusti-Tocco and Sato 1969;Rosenthal et al 1970). These cell lines were the first to exhibit differentiated traits of their tissues of origin .…”

Biomedical advances from tissue culture

“…An enrichment culture protocol that gave differentiated cells a selective advantage over fibroblasts was developed: functionally differentiated animal tumors were alternately passaged through cultures and animals multiple times (Buonassisi et al 1962). The rationale was that each passage in vitro selected culture-hardy variants of the differentiated tumor cells that were better able to compete with fibroblasts in culture.…”

“…The rationale was that each passage in vitro selected culture-hardy variants of the differentiated tumor cells that were better able to compete with fibroblasts in culture. Initially the Sato laboratory established steroid secreting and ACTH-responsive adrenal tumor cells and ACTH-secreting pituitary tumor cells (Buonassisi et al 1962). Within 10 years many other functional cell lines had been established with this method including growth hormone-secreting pituitary cells, antigen-specific glial cells, neurotransmitter-synthesizing neuroblastoma cells, differentiating teratoma cells, pigmented melanoma cells, and androgensecreting testicular cells.…”