Hormone dependence of breast cancer cells and the effects of tamoxifen and estrogen:31P NMR studies

Ruíz-Cabello, Jesús; Berghmans, Kirsten; Kaplan, Ofer; Lippman, Marc E.; Clarke, Robert; Cohen, Jack S.

doi:10.1007/bf00665945

Cited by 15 publications

(9 citation statements)

References 23 publications

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“…Indeed, the 3D and 2D constructs revealed differences in the fingerprints of the various cancer cell lines as well as different metabolic changes in response to chemotherapy. Treatment response studies have shown that cancer cells induced to form 3D spheroids are vastly less sensitive to chemotherapy than monolayer cells (30). In the current study, the LC 50 for CEM was higher by one order of magnitude than that of CGM.…”

Section: Discussioncontrasting

confidence: 45%

31P-Magnetic resonance spectra of ovarian cancer cells exposed to chemotherapy within a three-dimensional Matrigel construct

et al. 2012

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We aimed to determine the metabolic profile and effects of chemotherapy on ovarian cancer cell metabolism in a three-dimensional (3D) vs. a two-dimensional (2D) construct using 31P-magnetic resonance spectroscopy (MRS). Three ovarian cancer cell lines were embedded in a 3D perfused Matrigel construct or grown in a 2D monolayer. Metabolic differences between the three cell lines were determined using 31P-MRS both in the 3D and in the 2D constructs. Cells were incubated with three different cytotoxic drugs at LC50 for 44 h and evaluated for metabolic changes using 31P-MRS. While the 3D construct allowed MRS assessment of viable cells, the 2D monolayer permitted evaluation of non-viable cell extracts. In both cells embedded in Matrigel (CEM) and cells grown in monolayers (CGM) different cancer cell lines showed characteristic metabolic fingerprints, which differed significantly between CEM and CGM. In contrast to the cell monolayer, CEM allowed continuous monitoring of the changes in 31P-MRS spectra over time following exposure to chemotherapy, demonstrating a progressive decrease in specific phosphorylated metabolites. The metabolic response of CEM and CGM to various antimitotic agents was significantly different. We conclude that different ovarian cancer cell lines show characteristic 31P-MRS fingerprints and specific metabolic changes in response to cytotoxic drug treatment. The perfused 3D Matrigel construct is superior to the 2D tissue monolayer for 31P-MRS studies, because it simulates the in vivo conditions more closely and facilitates MRS evaluation of viable cells as well as continuous monitoring of metabolic changes in response to chemotherapy over time.

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Section: Discussioncontrasting

confidence: 45%

31P-Magnetic resonance spectra of ovarian cancer cells exposed to chemotherapy within a three-dimensional Matrigel construct

et al. 2012

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“…The similar cytotoxic profile of the crude extract against both HeLa (cervix cancer) and MCF-7 (breast cancer) cell lines followed by EACC might be attributed to their gynecological origin and hormone dependency [ 33 ]. Several gynecological solid tumors such as breast and cervix cancers are greatly influenced by hormone level and signaling within tumor micro milieu [ 34 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic and antioxidant properties of active principals isolated from water hyacinth against four cancer cells lines

Aboul‐Enein

Shanab

Shalaby

et al. 2014

BMC Complement Altern Med

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BackgroundEichhornia crassipes (Mart) solms is an invasive macrophyte causing serious problems to the network of irrigation and drainage canals in the Nile Delta region. The present study aim to evaluate the potential anticancer and antioxidant activities of Eichhornia crassipes crude extract and its pure compounds.MethodsThe macrophyte was collected from El-Zomor canal, River Nile (Egypt), cleaned, air dried, grinded then extracted with methanol (crude extract). The extract was fractionated using pre-coated silica gel plates (TLC F254) with hexane/ethyl acetate (8.5: 1.5 v/v) as mobile phase. Nine fractions were separated (A-I) then scratched, eluted with the same mobile phase, filtered and the separated fractions were determined and identified using spectroscopic methods (Mass spectrum (MS), Infra red (IR) and Proton H-Nuclear magnetic resonance (H-NMR). Both the crude extract and its nine identified compounds were tested for their antioxidant (using 2, 2 diphenyl-1-picrylhydrazyl (DPPH), 2, 2′- azino-bis {ethylbenzthiazoline-6-sulfonic acid (ABTS.)} methods) and anticancer activity (using MCF-7, HeLa, Hep.G2 and EACC cell lines).ResultsThe antioxidant and anticancer activities of the crude extract exhibited the highest effect while the compounds showed variable effects which depend on the type of compound and cancer cell line. The antioxidant activity of the crude extract exhibited the highest followed in descending order by compounds D, E, G and H respectively. Concerning the anticancer potency, the crude extract showed also the highest effect while the identified compounds (A, B, C, D, E, F, G, H and I) showed variable anticancer activities against the four different cell lines. In addition, Compound I exhibited the most potent anticancer activity against HepG2 cell line while compound D exhibited high anticancer activity against HeLa cells and EACC. The results revealed the presence of different compounds (Alkaloids and terpenoids) with variable antioxidant and anticancer activities which elicited an auto-augmentation in the crude extract leading to its greatest activities. The action of the identified anticancer compounds on DNA fragmentation was studied.ConclusionThe study illustrated the potential of Eichhornia as a valuable resource for natural compounds of desirable medicinal properties (e.g. antioxidants and anticancer).

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“…While it is the second leading cause of cancer mortality among females, the pathogenesis of the disease remains unclear [Kuller, 1995;Ernster et al, 1996]. In the malignant progression of breast cancer, breast tumours progress from hormone-dependent growth to a more aggressive phenotype characterised by hormone-independent growth, resistance to endocrine therapy, and a frequently widespread metastases [Leonessa et al, 1992;Ruiz Cabello et al, 1995].…”

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Nuclear matrix proteins in well and poorly differentiated human breast cancer cell lines

1997

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The nuclear matrix, besides providing the structural support of the nucleus, is involved in various cellular functions of the nucleus. Nuclear matrix proteins (NMPs), which are both tissue- and cell type-specific, are altered with transformation and state of differentiation. Furthermore, NMPs have been identified as informative markers of disease states. Here, the NMP profiles from human breast cancer cell lines and breast tumours were analyzed using two-dimension gel electrophoresis. We identified NMPs that are associated with well and poorly differentiated human breast cancer cells in vitro and in vivo. Five NMPs (NMBC 1-5) were found to be exclusive for well-differentiated human breast cancer cells, while one NMP (NMBC-6) was found to be present only in poorly differentiated human breast cancer cells. The identification of these proteins suggests the potential use of nuclear matrix proteins as prognostic indicators.

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Hormone dependence of breast cancer cells and the effects of tamoxifen and estrogen:31P NMR studies

Cited by 15 publications

References 23 publications

31P-Magnetic resonance spectra of ovarian cancer cells exposed to chemotherapy within a three-dimensional Matrigel construct

31P-Magnetic resonance spectra of ovarian cancer cells exposed to chemotherapy within a three-dimensional Matrigel construct

Cytotoxic and antioxidant properties of active principals isolated from water hyacinth against four cancer cells lines

Nuclear matrix proteins in well and poorly differentiated human breast cancer cell lines

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