Hormone biosynthesis and metabolism members of 2OGD superfamily are involved in berry development and respond to MeJA and ABA treatment of Vitis vinifera L

Abstract

Background Hormones play an indispensable role during fruit ripening, nine clades in 2-oxoglutarate-dependent dioxygenase (2OGD) superfamily are responsible for the hormone biosynthesis and metabolism, but less information is known about them. Results A total of 163 Vv2OGD superfamily members were identified from grape genome, which were mainly expanded by local (tandem and proximal) duplication. Phylogenetic analysis of 2OGD members in grape and Arabidopsis indicates 37 members in Vv2OGD superfamily are related to hormone biosynthesis and metabolism process (Vv2OGD-H), which could be divided into 9 clades, gibberellin (GA) 3-oxidase (GA3ox), GA 20-oxidase (GA20ox), carbon-19 GA 2-oxidase (C19-GA2ox), carbon-20 GA 2-oxidase (C20-GA2ox), 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), dioxygenase for auxin oxidation (DAO), lateral branching oxidoreductas (LBO), downy mildew resistant 6 and DMR6-like oxygenase (DMR6/DLO) and jasmonate-induced oxygenase (JOX). Sixteen of these 37 Vv2OGD-Hs are expressed in grape berry, in which the expression patterns of VvGA2oxs, VvDAOs and VvJOXs shows a correlation with the change patterns of GAs, indole-3-acetic acid (IAA) and jasmonates (JAs), indicating the involvement of these genes in grape berry development by regulating corresponding hormones. Twelve Vv2OGD-Hs respond to methyl JA (MeJA) treatment, of which eight may lead to the inhibition of the ripening process by the crosstalk of JAs-salicylic acids (SAs), JAs-GAs and JAs-JAs, while seven Vv2OGD-Hs respond to ABA treatment may be responsible for the promotion of ripening process by the interplay of abscisic acid (ABA)-strigolactones (SLs), ABA-SAs, ABA-GAs, ABA-JAs. Especially, VvLBO1 reach an expression peak near véraison and up-regulate about four times after ABA treatment, which implies SLs and ABA-SLs crosstalk may be related to the onset of berry ripening in grape. Conclusions This study provides valuable clues and new insights for the mechanism research of Vv2OGD-Hs in hormones regulation during the grape berry development.

Evolutionary and Integrative Analysis of the Gibberellin 20-oxidase, 3-oxidase, and 2-oxidase Gene Family in Paeonia ostii: Insight into Their Roles in Flower Senescence

“…Previous research has revealed that the JA/SA signaling pathways jointly downregulate genes associated with gibberellin, redirecting growth resources towards defense (Zhang et al, 2020) [50]. In V. vinifera, the transcript levels of VvGA2ox1a, VvGA2ox10 and VvGA2ox11 were upregulated significantly after MeJA treatment (Gao et al, 2022) [51]. Similarly, CaGA2ox1 was induced and upregulated expression by salicylic acid treatment in pepper (Lee et al, 2012) [52].…”

EBR and JA regulate aroma substance biosynthesis in ‘Ruidu Hongyu’ grapevine berries by transcriptome and metabolite combined analysis

“…According to prior research, EBR could significantly increase grape berry size and sugar content as well as improved grape color and flavor formation ( Li et al., 2022 ; Lv et al., 2022a ). Similarly, JA could also contribute to berry expansion, sugar accumulation, and anthocyanins formation ( Yu et al., 2020 ; Gao et al., 2022 ; Wang et al., 2022 ). In agreement with the results of previous research ( Li et al., 2022 ), this study further verified the results that EBR and JA also promoted the biochemical properties of ‘Ruidu Hongyu’ grapevine.…”

Correction: Hormone biosynthesis and metabolism members of 2OGD superfamily are involved in berry development and respond to MeJA and ABA treatment of Vitis vinifera L

“…Following publication of the original article [ 1 ], an error was identified in the presentation of the two authors. Gao Yingying and Wang Xiaochen should be presented as Yingying Gao and Xiaochen Wang.…”