Hormonally Active Agents: A Menace for Oogenesis and Fertility in Teleosts

Biswas, Subhasri; Ghosh, Soumyajyoti; Mukherjee, Urmi; Samanta, Anwesha; Das, Sriparna; Maitra, Saumen Kumar

doi:10.1007/978-981-15-8369-8_19

Cited by 2 publications

(2 citation statements)

References 184 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown that Bisphenol A may adversely affect follicle formation and affect the healthy development of reproductive organs in chickens (Mentor et al, 2020;Eldefrawy et al, 2021). In addition, it has been shown that Bisphenol A may impair the reproductive adaptations of zebrafish ovaries (Biswas et al, 2020). Cortisol is a glucocorticoid with several physiological functions, such as: response to stress, regulation of apoptosis and lipid metabolism.…”

Section: Metabolomics Analysismentioning

confidence: 99%

Transcriptomic and metabolomic analyses of the ovaries of Taihe black-bone silky fowls at the peak egg-laying and nesting period

Xiang,

Huang,

Wang

et al. 2023

Front. Genet.

View full text Add to dashboard Cite

The poor reproductive performance of most local Chinese chickens limits the economic benefits and output of related enterprises. As an excellent local breed in China, Taihe black-bone silky fowl is in urgent need of our development and utilization. In this study, we performed transcriptomic and metabolomic analyses of the ovaries of Taihe black-bone silky fowls at the peak egg-laying period (PP) and nesting period (NP) to reveal the molecular mechanisms affecting reproductive performance. In the transcriptome, we identified five key differentially expressed genes (DEGs) that may affect the reproductive performance of Taihe black-bone silky fowl: BCHE, CCL5, SMOC1, CYTL1, and SCIN, as well as three important pathways: the extracellular region, Neuroactive ligand-receptor interaction and Cytokine-cytokine receptor interaction. In the metabolome, we predicted three important ovarian significantly differential metabolites (SDMs): LPC 20:4, Bisphenol A, and Cortisol. By integration analysis of transcriptome and metabolome, we identified three important metabolite-gene pairs: “LPC 20:4-BCHE”, “Bisphenol A-SMOC1”, and “Cortisol- SCIN”. In summary, this study contributes to a deeper understanding of the regulatory mechanism of egg production in Taihe black-bone silky fowl and provides a scientific basis for improving the reproductive performance of Chinese local chickens.

show abstract

Section: Metabolomics Analysismentioning

confidence: 99%

Transcriptomic and metabolomic analyses of the ovaries of Taihe black-bone silky fowls at the peak egg-laying and nesting period

Xiang,

Huang,

Wang

et al. 2023

Front. Genet.

View full text Add to dashboard Cite

show abstract

“…Reconstitution of RNA pellet was followed by quantification, and integrity check was performed using Multiskan Go microplate spectrophotometer (Thermo Scientific, India). 1 μg of total RNA from each treatment group pre-treated with 1 U of DNase I was reverse transcribed at 42 °C for 1 h using Verso cDNA synthesis kit (#AB1453, Thermo Scientific), and qPCRs were set up on a Quant-Studio-5 Real-Time PCR System (Applied Biosystems) (Biswas et al, 2020) using gene-specific primers from earlier reports (Supplementary Table S2). The reaction mix for both target and reference genes contained cDNA template (diluted 1:10), primers (250 nM each for forward and reverse) and SYBR Green PCR Master Mix (Applied Biosystems).…”

Section: Ros Determination By Dihydroethidium (Dhe) Oxidation Assaymentioning

confidence: 99%

Role of insulin-like growth factor 1 (IGF1) in the regulation of mitochondrial bioenergetics in zebrafish oocytes: lessons from in vivo and in vitro investigations

Biswas

Ghosh

Maitra

2023

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Optimal mitochondrial functioning is indispensable for acquiring oocyte competence and meiotic maturation, whilst mitochondrial dysfunction may lead to diminished reproductive potential and impaired fertility. The role of the intra-ovarian IGF system in ovarian follicular dynamics has been implicated earlier. Although several studies have demonstrated the role of the IGF axis in facilitating mitochondrial function over a multitude of cell lines, its role in oocyte energy metabolism remains largely unexplored. Here using zebrafish, the relative importance of IGF1 in modulating oocyte mitochondrial bioenergetics has been investigated. A dramatic increase in ovarian lhcgr and igf1 expression accompanied heightened ATP levels and mitochondrial polarization in full-grown (FG) oocytes resuming meiotic maturation and ovulation in vivo. Concomitant with elevated igf1 expression and IGF1R phosphorylation, hCG (LH analog) stimulation of FG follicles in vitro prompted a sharp increase in NRF-1 and ATP levels, suggesting a positive influence of gonadotropin action on igf1 expression vis-à-vis oocyte bioenergetics. While recombinant IGF1 administration enhanced mitochondrial function, IGF1R immunodepletion or priming with PI3K inhibitor wortmannin could abrogate NRF-1 immunoreactivity, expression of respiratory chain subunits, ΔΨM, and ATP content. Mechanistically, activation of PI3K/Akt signaling in IGF1-treated follicles corroborated well with the rapid phosphorylation of GSK3β at Ser9 (inactive) followed by PGC-1β accumulation. While selective inhibition of GSK3β promoted PGC-1β, Akt inhibition could abrogate IGF1-induced p-GSK3β (Ser9) and PGC-1β immunoreactive protein indicating Akt-mediated GSK3β inactivation and PGC-1β stabilization. The IGF1-depleted follicles showed elevated superoxide anions, subdued steroidogenic potential, and attenuated G2-M1 transition. In summary, this study highlights the importance of IGF1 signaling in oocyte bioenergetics prior to resumption of meiosis.

show abstract

Hormonally Active Agents: A Menace for Oogenesis and Fertility in Teleosts

Cited by 2 publications

References 184 publications

Transcriptomic and metabolomic analyses of the ovaries of Taihe black-bone silky fowls at the peak egg-laying and nesting period

Transcriptomic and metabolomic analyses of the ovaries of Taihe black-bone silky fowls at the peak egg-laying and nesting period

Role of insulin-like growth factor 1 (IGF1) in the regulation of mitochondrial bioenergetics in zebrafish oocytes: lessons from in vivo and in vitro investigations

Contact Info

Product

Resources

About