Hormonal Sensitivity of Brown Fat Cells of Fetal Rats in Monolayer Culture

Sugihara, Hajime; Miyabara, Shinichi; Yonemitsu, Nobuhisa; Ohta, K.

doi:10.1055/s-0029-1210291

Exp Clin Endocrinol Diabetes

2009

DOI: 10.1055/s-0029-1210291

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Hormonal Sensitivity of Brown Fat Cells of Fetal Rats in Monolayer Culture

Hajime Sugihara¹,

Shinichi Miyabara²,

Nobuhisa Yonemitsu³

et al.

Abstract: The primary monolayer culture of brown fat cells was performed with the brown adipose tissue of fetal rats as the material. Brown fat cells in the culture were highly sensitive to some hormones. Insulin had a marked effect of lipogenesis and it made the cells contain large multilocular lipid droplets. Lipolysis is also mediated by hormones, e.g., epinephrine or ACTH etc., and lipolytic process was controlled through cyclic AMP system. Brown fat cells of fetal rats in monolayer culture seem to be a suitable mat… Show more

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Cited by 16 publications

References 4 publications

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Functional and structural differences between cultured outer and inner layer cells of bovine adrenal cortex

Yonemitsu

1987

Cell Tissue Res.

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Outer and inner layer cells of bovine adrenal cortex were cultured separately to compare cellular structural characteristics and functional differences. Outer layer cells were polygonal in shape with radially distributed lipid droplets in the cytoplasm, and produced mainly aldosterone and cortisol. The aldosterone production increased upon stimulation with angiotensin II or dibutyryl-cAMP. In contrast, inner layer cells were spindle-shaped and had fine diffused lipid droplets. They produced four times as much cortisol as outer layer cells but no aldosterone. Cortisol production increased with ACTH or dibutyryl-cAMP stimulation. When stimulated by ACTH or by dibutyryl-cAMP, both types of adrenocortical cells showed "cellular retraction" whereby the number of cytoplasmic lipid droplets decreased and microvilli on the cellular surface increased. At the same time, the transverse distribution of actin fibers disappeared and the microtubules changed their distribution pattern from circular to radial. Stimulation by angiotensin II, on the other hand, brought no marked structural changes. These results indicate that, in functional terms, the outer layer cells and the inner layer cells in this culture system reflect zona glomerulosa and zona fasciculata-reticularis, respectively.

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Functional and structural differences between cultured outer and inner layer cells of bovine adrenal cortex

Yonemitsu

1987

Cell Tissue Res.

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Immunocytochemistry of the microtubules of fat-laden cells

et al. 1983

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Cytoplasmic microtubules of fat-laden cells containing fine lipid droplets, such as brown fat cells and adrenocortical cells, were studied in relation to the metabolism of intracellular lipid. In these cells, the amount and distribution of lipid droplets reflect the state of inherent cellular function. Materials used were primary monolayer culture of fetal rat brown fat cells and that of bovine adrenocortical cells. The method was the immunocytochemistry with anti-tubulin antibody. When brown fat cells were being lipolyzed or the steroidogenesis of adrenocortical cells were being stimulated, the cytoplasmic microtubules in the cells were organized in a radial pattern in response to the behavior of the lipid droplets. It is assumed that the microtubules were in the regulation of cellular function in terms of the metabolism of lipid droplets in these cells. We have devised, in the course of the current study, a double fluorescence technique as an observational method whereby microtubules were observed immunocytochemically and lipid droplets by a secondary fluorescence with phosphine E staining.

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A preadipocyte clonal line from mouse brown adipose tissue

Forest

Doglio

Ricquier

et al. 1987

Experimental Cell Research

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Hormonal Sensitivity of Brown Fat Cells of Fetal Rats in Monolayer Culture

Cited by 16 publications

References 4 publications

Functional and structural differences between cultured outer and inner layer cells of bovine adrenal cortex

Functional and structural differences between cultured outer and inner layer cells of bovine adrenal cortex

Immunocytochemistry of the microtubules of fat-laden cells

A preadipocyte clonal line from mouse brown adipose tissue

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