Hormonal regulation of H19 gene expression in prostate epithelial cells

Berteaux, Nathalie; Lottin, Séverine; Adriaenssens, Éric; Coppenolle, Fabien Van; Leroy, Xavier; Cottet, J; Dugimont, Thierry; Curgy, Jean-Jacques

doi:10.1677/joe.1.05696

Cited by 30 publications

(16 citation statements)

References 50 publications

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“…We observed significant increased expression in RW-GFP cells for all of them, with p63 expression being the most important (*18-fold). The long noncoding (lnc) RNA H19 is involved in prostate carcinogenesis [29] and plays a role in early development [23]. These results led us to examine the expression of the noncoding RNA H19 in RW-GFP cells.…”

Section: Fig 3 Chemoresistance Of Rw-gfp Cells (A B)mentioning

confidence: 99%

Enrichment of Human Stem-Like Prostate Cells with s-SHIP Promoter Activity Uncovers a Role in Stemness for the Long Noncoding RNA H19

Roy

Vennin

Brocqueville

et al. 2015

Stem Cells and Development

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Understanding normal and cancer stem cells should provide insights into the origin of prostate cancer and their mechanisms of resistance to current treatment strategies. In this study, we isolated and characterized stem-like cells present in the immortalized human prostate cell line, RWPE-1. We used a reporter system with green fluorescent protein (GFP) driven by the promoter of s-SHIP (for stem-SH2-domain-containing 5¢-inositol phosphatase) whose stem cell-specific expression has been previously shown. We observed that s-SHIP-GFPexpressing RWPE-1 cells showed stem cell characteristics such as increased expression of stem cell surface markers (CD44, CD166, TROP2) and pluripotency transcription factors (Oct4, Sox2), and enhanced sphereforming capacity and resistance to arsenite-induced cell death. Concomitant increased expression of the long noncoding RNA H19 was observed, which prompted us to investigate a putative role in stemness for this oncofetal gene. Targeted suppression of H19 with siRNA decreased Oct4 and Sox2 gene expression and colonyforming potential in RWPE-1 cells. Conversely, overexpression of H19 significantly increased gene expression of these two transcription factors and the sphere-forming capacity of RWPE-1 cells. Analysis of H19 expression in various prostate and mammary human cell lines revealed similarities with Sox2 expression, suggesting that a functional relationship may exist between H19 and Sox2. Collectively, we provide the first evidence that s-SHIP-GFP promoter reporter offers a unique marker for the enrichment of human stem-like cell populations and highlight a role in stemness for the long noncoding RNA H19.

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Section: Fig 3 Chemoresistance Of Rw-gfp Cells (A B)mentioning

confidence: 99%

Enrichment of Human Stem-Like Prostate Cells with s-SHIP Promoter Activity Uncovers a Role in Stemness for the Long Noncoding RNA H19

Roy

Vennin

Brocqueville

et al. 2015

Stem Cells and Development

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“…Another member of the PCAT family, PCAT-18 was upregulated after DHT treatment, although this effect was suggested to be due to the activation of upstream transcription factors (Crea et al, 2014). In contrast, the lncRNA H19 was downregulated after DHT treatment in LNCaP cells (Berteaux et al, 2004). Also, expression of PCAT-29 (prostate cancerassociated transcript 29) under the control of AR binding to its promoter was reduced after DHT treatment in ARpositive cell lines (Malik et al, 2014).…”

Section: Lncrnas Regulated By Ar Activationmentioning

confidence: 99%

Role of lncRNAs in prostate cancer development and progression

Weiß

Plass

Gerhäuser

2014

Biological Chemistry

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Prostate cancer (PCa) is the second most common cause of cancer-related deaths in men. Despite advances in the characterization of genomic and epigenetic aberrations contributing to PCa, the etiology of PCa is still far from being understood. Research over the past decade demonstrated the role of long non-coding RNAs (lncRNAs) in deregulation of target genes mainly through epigenetic mechanisms. In PCa, evidence accumulated that hundreds of lncRNAs are dysregulated. Functional analyses revealed their contribution to prostate carcinogenesis by targeting relevant pathways and gene regulation mechanisms including PTEN/AKT and androgen receptor signaling as well as chromatin remodeling complexes. Here we summarize our current knowledge on the roles of lncRNAs in PCa and their potential use as biomarkers for aggressive PCa and as novel therapeutic targets.

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“…If the imprinted interactome persists in adult prostatic cells, H19 and other imprinted genes might be influenced by androgen responses in prostate cancer. 122 Taken together, these findings infer that relaxed IGF/H19 imprinting may occur early in prostate carcinogenesis where environmental factors or ageing may influence its epigenetic state. One could speculate that the resulting overexpression of IGF2 and H19 may promote the enlargement of an epigenetically unstable but not neoplastic progenitor cell pool and skew the androgen response, thereby changing the microenvironment and facilitating cancer formation.…”

Section: Insulin-like Growth Factor 2 (Igf2)mentioning

confidence: 81%

Specific changes in the expression of imprinted genes in prostate cancer—implications for cancer progression and epigenetic regulation

Ribarska¹,

Bastian²,

Koch³

et al. 2012

Asian J Androl

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Epigenetic dysregulation comprising DNA hypermethylation and hypomethylation, enhancer of zeste homologue 2 (EZH2) overexpression and altered patterns of histone modifications is associated with the progression of prostate cancer. DNA methylation, EZH2 and histone modifications also ensure the parental-specific monoallelic expression of at least 62 imprinted genes. Although it is therefore tempting to speculate that epigenetic dysregulation may extend to imprinted genes, expression changes in cancerous prostates are only well documented for insulin-like growth factor 2 (IGF2). A literature and database survey on imprinted genes in prostate cancer suggests that the expression of most imprinted genes remains unchanged despite global disturbances in epigenetic mechanisms. Instead, selective genetic and epigenetic changes appear to lead to the inactivation of a sub-network of imprinted genes, which might function in the prostate to limit cell growth induced via the PI3K/Akt pathway, modulate androgen responses and regulate differentiation. Whereas dysregulation of IGF2 may constitute an early change in prostate carcinogenesis, inactivation of this imprinted gene network is rather associated with cancer progression.

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Hormonal regulation of H19 gene expression in prostate epithelial cells

Cited by 30 publications

References 50 publications

Enrichment of Human Stem-Like Prostate Cells with s-SHIP Promoter Activity Uncovers a Role in Stemness for the Long Noncoding RNA H19

Enrichment of Human Stem-Like Prostate Cells with s-SHIP Promoter Activity Uncovers a Role in Stemness for the Long Noncoding RNA H19

Role of lncRNAs in prostate cancer development and progression

Specific changes in the expression of imprinted genes in prostate cancer—implications for cancer progression and epigenetic regulation

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